mkn 45 Search Results


90
CLS Cell Lines Service GmbH mkn45
Mkn45, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc11880418-350-0-3?v=CLS+Cell+Lines+Service+GmbH
Average 90 stars, based on 1 article reviews
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mkn45  (DSMZ)
95
DSMZ mkn45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn45, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc12917973-58-19-20?v=DSMZ
Average 95 stars, based on 1 article reviews
mkn45 - by Bioz Stars, 2026-06
95/100 stars
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93
AcceGen Biotechnology mkn 45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc12389611-39-0-1?v=AcceGen+Biotechnology
Average 93 stars, based on 1 article reviews
mkn 45 - by Bioz Stars, 2026-06
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86
Procell Inc mkn 45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pm41144105-38-14-16?v=Procell+Inc
Average 86 stars, based on 1 article reviews
mkn 45 - by Bioz Stars, 2026-06
86/100 stars
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90
Biopharm GmbH mkn-45 gc cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Gc Cells, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc06176415-36-3-10?v=Biopharm+GmbH
Average 90 stars, based on 1 article reviews
mkn-45 gc cells - by Bioz Stars, 2026-06
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90
Egens Biotechnology human gastric cancer mkn-45 cell line
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Human Gastric Cancer Mkn 45 Cell Line, supplied by Egens Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pm24137432-22-35-70?v=Egens+Biotechnology
Average 90 stars, based on 1 article reviews
human gastric cancer mkn-45 cell line - by Bioz Stars, 2026-06
90/100 stars
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90
Beijing Xiehe Pharmaceutical Co Ltd human gc cells mkn-45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Human Gc Cells Mkn 45, supplied by Beijing Xiehe Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc11833373-58-7-17?v=Beijing+Xiehe+Pharmaceutical+Co+Ltd
Average 90 stars, based on 1 article reviews
human gc cells mkn-45 - by Bioz Stars, 2026-06
90/100 stars
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90
Shanghai GenePharma lentivirus transfected mkn-45 cells (shcpne1 or shnc)
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Lentivirus Transfected Mkn 45 Cells (Shcpne1 Or Shnc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pm35985444-124-7-15?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
lentivirus transfected mkn-45 cells (shcpne1 or shnc) - by Bioz Stars, 2026-06
90/100 stars
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90
SMAC Corp mkn-45/smac cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45/Smac Cells, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc04205389-149-10-2?v=SMAC+Corp
Average 90 stars, based on 1 article reviews
mkn-45/smac cells - by Bioz Stars, 2026-06
90/100 stars
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90
Chemon Inc mkn-45 cell line
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Cell Line, supplied by Chemon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/us10441582-77-3-14?v=Chemon+Inc
Average 90 stars, based on 1 article reviews
mkn-45 cell line - by Bioz Stars, 2026-06
90/100 stars
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90
Technical Manufacturing Company mkn-45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by Technical Manufacturing Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc04240476-34-11-7?v=Technical+Manufacturing+Company
Average 90 stars, based on 1 article reviews
mkn-45 - by Bioz Stars, 2026-06
90/100 stars
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90
Allogen Laboratories mkn-45 gc cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Gc Cells, supplied by Allogen Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mkn+45/pmc05405592-37-8-30?v=Allogen+Laboratories
Average 90 stars, based on 1 article reviews
mkn-45 gc cells - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, MKN45, St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM

Journal: BMC Cancer

Article Title: The Gb3-synthase A4GALT is an epigenetically regulated driver of tumor invasiveness in gastrointestinal cancer

doi: 10.1186/s12885-026-15600-7

Figure Lengend Snippet: Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, MKN45, St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM

Article Snippet: The cell lines used in this study were the gastric cancer lines St3051 [ ], AGS (ATCC, CRL-1739), and MKN45 (DSMZ, ACC 409), and the colorectal cancer lines HCT116 (ATCC, CCL-247), DLD1 (ATCC, CCL-221).

Techniques: Expressing, Staining, Immunofluorescence, Microscopy, Control, Incubation