Journal: Chemistry & biology
Article Title: Red-Shifted Voltage Sensitive Fluorescent Proteins
Figure Lengend Snippet: Expression and functional validation of red-shifted VSFP3.1-based voltage sensors in neuronal cultures (A–D) Confocal fluorescence imaging of primary hippocampal cultures expressing VSFP3.1_Citrine (A), VSFP3.1_TagRFP (B), VSFP3.1_mKate2 (C) or VSFP3.1_mOrange2 (D). Magnified views of boxed regions are shown in inserts. Scale bars: 40 µm (overviews) and 10 µm (inserts). (E) Optical recordings of spontaneous electrical activity in primary neuronal cultures derived from mouse hippocampus. The neurons were transfected with VSFP3.1_mOrange2 and the recordings were done under current-clamp conditions at 35°C. Asterisks indicate individual action potentials that were resolved optically in single sweeps (arrows).
Article Snippet: TagRFP and mKate2 emission spectra were obtained from Evrogen website (
Techniques: Expressing, Functional Assay, Fluorescence, Imaging, Activity Assay, Derivative Assay, Transfection