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    Evrogen mkate2 emission spectra
    Expression and functional validation of red-shifted VSFP3.1-based voltage sensors in neuronal cultures (A–D) Confocal fluorescence imaging of primary hippocampal cultures expressing VSFP3.1_Citrine (A), VSFP3.1_TagRFP (B), <t>VSFP3.1_mKate2</t> (C) or VSFP3.1_mOrange2 (D). Magnified views of boxed regions are shown in inserts. Scale bars: 40 µm (overviews) and 10 µm (inserts). (E) Optical recordings of spontaneous electrical activity in primary neuronal cultures derived from mouse hippocampus. The neurons were transfected with VSFP3.1_mOrange2 and the recordings were done under current-clamp conditions at 35°C. Asterisks indicate individual action potentials that were resolved optically in single sweeps (arrows).
    Mkate2 Emission Spectra, supplied by Evrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression and functional validation of red-shifted VSFP3.1-based voltage sensors in neuronal cultures (A–D) Confocal fluorescence imaging of primary hippocampal cultures expressing VSFP3.1_Citrine (A), VSFP3.1_TagRFP (B), VSFP3.1_mKate2 (C) or VSFP3.1_mOrange2 (D). Magnified views of boxed regions are shown in inserts. Scale bars: 40 µm (overviews) and 10 µm (inserts). (E) Optical recordings of spontaneous electrical activity in primary neuronal cultures derived from mouse hippocampus. The neurons were transfected with VSFP3.1_mOrange2 and the recordings were done under current-clamp conditions at 35°C. Asterisks indicate individual action potentials that were resolved optically in single sweeps (arrows).

    Journal: Chemistry & biology

    Article Title: Red-Shifted Voltage Sensitive Fluorescent Proteins

    doi: 10.1016/j.chembiol.2009.11.014

    Figure Lengend Snippet: Expression and functional validation of red-shifted VSFP3.1-based voltage sensors in neuronal cultures (A–D) Confocal fluorescence imaging of primary hippocampal cultures expressing VSFP3.1_Citrine (A), VSFP3.1_TagRFP (B), VSFP3.1_mKate2 (C) or VSFP3.1_mOrange2 (D). Magnified views of boxed regions are shown in inserts. Scale bars: 40 µm (overviews) and 10 µm (inserts). (E) Optical recordings of spontaneous electrical activity in primary neuronal cultures derived from mouse hippocampus. The neurons were transfected with VSFP3.1_mOrange2 and the recordings were done under current-clamp conditions at 35°C. Asterisks indicate individual action potentials that were resolved optically in single sweeps (arrows).

    Article Snippet: TagRFP and mKate2 emission spectra were obtained from Evrogen website (

    Techniques: Expressing, Functional Assay, Fluorescence, Imaging, Activity Assay, Derivative Assay, Transfection

    Expression and voltage sensing characteristics of VSFP3.1 color variants in PC12 cells (A) Fluorescence and transmission images of PC12 cells expressing VSFP3.1_Cerulean, VSFP3.1_Citrine, VSFP3.1_mOrange2, VSFP3.1_TagRFP and VSFP3.1_mKate2. Scale bars: 20 µm. (B) Fluorescence signals in response to test potentials of −140 to 60 mV from a holding potential of −70 mV at 35°C

    Journal: Chemistry & biology

    Article Title: Red-Shifted Voltage Sensitive Fluorescent Proteins

    doi: 10.1016/j.chembiol.2009.11.014

    Figure Lengend Snippet: Expression and voltage sensing characteristics of VSFP3.1 color variants in PC12 cells (A) Fluorescence and transmission images of PC12 cells expressing VSFP3.1_Cerulean, VSFP3.1_Citrine, VSFP3.1_mOrange2, VSFP3.1_TagRFP and VSFP3.1_mKate2. Scale bars: 20 µm. (B) Fluorescence signals in response to test potentials of −140 to 60 mV from a holding potential of −70 mV at 35°C

    Article Snippet: TagRFP and mKate2 emission spectra were obtained from Evrogen website (

    Techniques: Expressing, Fluorescence, Transmission Assay