Journal: Oxidative Medicine and Cellular Longevity

Article Title: Triterpenoid Saponin AG8 from Ardisia gigantifolia stapf. Induces Triple Negative Breast Cancer Cells Apoptosis through Oxidative Stress Pathway

doi: 10.1155/2020/7963212

Figure Lengend Snippet: Effects of AG8 on mitochondrial function in TNBC cells. (a) MDA-MB-231, BT-549, and MDA-MB-157 cells were incubated with 0.1% DMSO or with AG8 for 24 h. Following treatment, cells were incubated with 100 nM MitoTracker Red CMXRos for 30 min. The cells were observed by fluorescence microscope (100x). (b) Intracellular ROS generation induced by AG8 and NAC was measured by DCFH-DA (10 μ M) and flow cytometry. All results are representative of three independent experiments, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 versus control.

Article Snippet: MDA-MB-231, MDA-MB-157, and BT-549 cells were incubated with different concentrations of AG8 for 24 h, and then, 100 nM MitoTracker Red CMXRos was added to the cells (Keygen, Jiangsu, China).

Techniques: Incubation, Fluorescence, Microscopy, Flow Cytometry, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence

doi: 10.1155/2020/5367102

Figure Lengend Snippet: PUVA-induced mitochondrial mass increase and the suppression by ribose-5-phosphate. (a–c) The mitochondrial mass in control (a) and PUVA-treated (at week 3 and week 6) fibroblasts (b, c) determined by MitoTracker Red; (d) determination of mitochondrial membrane potential ( ΔΨ ) by a polarization-sensitive fluorescent dye JC-9 based on the ratio between two ΔΨ -driven interchangeable forms, monomer (J-monomer, green) and dimer (J-aggregate, red); (e, f) the cell morphology after PUVA (at week 3) without (e) and with ribose-5-phosphate (5 mM, f).

Article Snippet: Mitochondrial mass was determined by staining living cells with 25 nM of the fluorescent dye MitoTracker Red CMXRos (MoBiTec, Goettingen) in DMEM for 30 min at 37°C, then photograph using a 568 nm line of fluorescence excitation with a laser scanning confocal microscope.

Techniques: Control, Membrane

Journal: Stem Cell Research

Article Title: Metabolic flux analyses to assess the differentiation of adult cardiac progenitors after fatty acid supplementation

doi: 10.1016/j.scr.2019.101458

Figure Lengend Snippet: Long-term stimulation with OA: (A) P4 CPCs treated with OA for 48 h (4a), 1 week (4b) and 1 month (4c), as well as untreated CPCs grown for 1 month (scale bars: 100 μm). (B) Effect of 48 h, 1 week, 1 month supplementation of P4 CPCs with OA on the expression of metabolic genes, normalised to control untreated CPCs. (n = 3; error bars: standard error, * p < .05, #p < .02). (C) Effect of OA treatment for 48 h and 1 week, estimated as average fluorescent intensity, after staining with MitoTracker Red CMXRos red. * p < 0,00001 normalised to untreated CNTRL, and OA 48-h treatment, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For mitochondrial imaging, the MitoTracker® Red CMXRos (Fisher Scientific – UK Ltd) intracellular dye was used, following manufacturer's protocol.

Techniques: Expressing, Control, Staining

Journal: Stem Cell Research

Article Title: Metabolic flux analyses to assess the differentiation of adult cardiac progenitors after fatty acid supplementation

doi: 10.1016/j.scr.2019.101458

Figure Lengend Snippet: OA supplementation affects substrate metabolism in differentiated CPCs: (A) Metabolic effect of 1 week supplementation with OA post-differentiation on gene expression in CPCs, compared to non-treated differentiated CPCs and CNTRL undifferentiated CPCs. (n = 4, *p < 0,05, $p < 0,01 indicating difference to CNTRL, error bars: standard error). (B) Effect of differentiation, and differentiation followed by 1 week of OA 300 μM, on cell mitochondria, estimated as average fluorescent intensity, after staining with MitoTracker Red CMXRos red. ( n = 12 cells, 3 replicates, * p < .001). (C) Effect of TGF-β1 differentiation, and differentiation followed by 1 week supplementation with OA, on the glucose oxidation levels, (D) Glycolysis, and E) FA Oxidation, measured as nMoles/ min/ 10 5 cells, normalised to control untreated CPCs. (n = 3; * p < .03, #p < .01, $p < .001 indicating differences amongst the groups, error bars: standard error). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For mitochondrial imaging, the MitoTracker® Red CMXRos (Fisher Scientific – UK Ltd) intracellular dye was used, following manufacturer's protocol.

Techniques: Gene Expression, Staining, Control

Journal: BMC Complementary Medicine and Therapies

Article Title: Structure based docking and biological evaluation towards exploring potential anti-cancerous and apoptotic activity of 6-Gingerol against human prostate carcinoma cells

doi: 10.1186/s12906-023-04269-1

Figure Lengend Snippet: Intracellular ROS accumulation in PC-3 cells with DCFH-DA staining after 6-G treatment. A Photomicrograph showed ROS production intracellularly induced by 80, 100 and 120 µM of 6-Gingerol. Pictures were analysed phase contrast microscope. B Data are expressed numerically as fluorescence intensity percentage compared to control of PC-3 cells. Means ± SEM values are expressed of minimum three experiments independently, ** P < 0.01, *** p <0.001 and **** p <0.0001 in comparison to their particular control

Article Snippet: Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye, 4′,6-diamidino-2-phenylindole (DAPI), 2′,7′-Dichlorofluorescein diacetate (DCFH-DA), MitoTracker Red CMXRos, Acridine Orange (AO) and Propidium Iodide (PI) were purchased from Himedia, India for anticancer activity assessment.

Techniques: Staining, Microscopy, Fluorescence, Control, Comparison

Journal: Microbiology Spectrum

Article Title: The mitochondrial protein Bcs1A regulates antifungal drug tolerance by affecting efflux pump expression in the filamentous pathogenic fungus Aspergillus fumigatus

doi: 10.1128/spectrum.01172-24

Figure Lengend Snippet: Mitochondria-localized Bcs1A is required for hyphal growth in A. fumigatus . ( A ) Bioinformatic analysis of selected Bcs1 homologs from eukaryotes. The phylogenetic tree was constructed using MEGA 7 software. Domains were analyzed using SMART ( http://smart.embl-heidelberg.de/ ). ( B ) Subcellular localization of green fluorescent protein (GFP)-tagged Bcs1A. MitoTracker was used to visualize mitochondria. Scale bar, 10 µm. Colony phenotypes ( C ) and diameters ( D ) of the wild-type (WT), Δ bcs1A , and bcs1A C strains grown in minimal medium (MM) and yeast extract-glucose (YG) media at 37°C for 48 h. ns, not significant; ***, P < 0.001.

Article Snippet: To visualize the subcellular distribution of Bcs1A, the GFP-labeled strain was incubated in 500 μL of liquid MM on glass coverslips for a duration of 12 h. Samples were fixed in 4% formaldehyde for 30 min and then incubated in the dark with the mitochondrial dye MitoTracker Red CMXRos (Beyotime) for 5 min used at a final concentration of 5 nM.

Techniques: Construct, Software