Journal: iScience

Article Title: NOT gated T cells that selectively target EGFR and other widely expressed tumor antigens

doi: 10.1016/j.isci.2024.109913

Figure Lengend Snippet:

Article Snippet: Mitomycin C , Thermo scientific chemicals , Cat# AAJ67460XF.

Techniques: Purification, Virus, Recombinant, Cell Isolation, Staining, Control, TaqMan Assay, Luciferase, Sequencing, Construct, Software

Journal: Vaccine

Article Title: Immunogenicity of RepliVAX WN, a novel single-cycle West Nile virus vaccine.

doi: 10.1016/j.vaccine.2010.10.069

Figure Lengend Snippet: Fig. 4. Antigen-specificity and effector function of CD4+ T cells to RepliVAX WN immunization. Effect of immunization with 104 or 106 IU RepliVAX WN on induction of a WNV NS32066- (A) and E347-specific (B) CD4+ T cell response. (*P < 0.05: ANOVA, versus naïve controls.) (C) Kinetics and epitope-specificity of the CD4+ T cell response following RepliVAX WN immunization. B6 mice were immunized with 106 IU RepliVAX WN and splenocytes were stimulated with the indicated WNV peptide and the number of IFN- secreting cells from 5 mice/group quantified by ELISPOT on the indicated day. Epitope-specific values from immunized mice were compared to values from non-immune cells (day 0) stimulated with the same peptide. **P < 0.001, *P < 0.05; Student t-test. Results of a representative experiment of 2 performed are shown. (D) Kinetics of the CD4+ cytotoxic T lymphocyte response following RepliVAX WN immunization of B6 mice. RepliVAX WN immunized mice (n = 5) were assessed for cytolytic activity on the indicated day post-immunization. Preparation of targets and detection and calculation of cytotoxicity were performed as described in Section 2. Comparisons were made between immune and naïve mice injected with the same target cells. **P < 0.0001, *P < 0.005; Student t-test. Results are compiled from 2 separate experiments; n = 6/group.

Article Snippet: Serial dilutions of splenocytes from naïve or RepliVAX WN immunized animals were plated with mitomycin C-treated B6 splenocytes previously pulsed with immunogenic WNV peptides, and incubated at 37 ◦C for 40 h. The following immunogenic peptides representing WNV CD4+ and CD8+ T cell epitopes have been reported previously [15,16,18] and were synthesized (New England Peptide, Gardner, MA) and used for stimulation: CD8+ T cell epitopes; E protein amino acid (AA) residues 347- 354, RSYCYLAT (E347); NS4B protein AA residues 2488–2496, SSVWNATTA (NS4B2488) or CD4+ T cell epitopes; E protein AA residues 431–445, IFVHGPTTVESHGNY (E431); E protein AA residues 641–655, PVGRLVTVNPFVSVA (E641); NS3 protein AA residues 1616–1630, TKPGVFKTPEGEIGA (NS31616); NS3 protein AA residues 2066–2080, RRWCFDGPRTNTILE (NS32066).

Techniques: Enzyme-linked Immunospot, Activity Assay, Injection