Journal: PLoS ONE

Article Title: Aberrant Apoptotic Response of Colorectal Cancer Cells to Novel Nucleoside Analogues

doi: 10.1371/journal.pone.0138607

Figure Lengend Snippet: Summary of the mitochondrial membrane potential change induced by the nucleoside analogues and camptothecin in the HT-29 and Caco-2 colon cancer cell lines.

Article Snippet: The cytosolic fraction was obtained by resuspending the cell pellet with a cell permeabilization buffer, (250 mM sucrose, 137 mM NaCl, 70 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM K 2 HPO 4 , 0.2 mg/ml Digitonin and 0.1% Hydorol M) supplied in the Mitochondrial isolation kit, (Assay Designs Inc.) which left the mitochondria intact.

Techniques: Membrane, Analogues

Journal: Journal of Pesticide Science

Article Title: Comparative hepatotoxicity of a herbicide, epyrifenacil, in humans and rodents by comparing the dynamics and kinetics of its causal metabolite

doi: 10.1584/jpestics.D21-026

Figure Lengend Snippet: Table 3. Inhibitory activity (IC 50 values, [nM]) of epyrifenacil and S-3100-CA against mitochondrial PPO fractions in each species.

Article Snippet: For mice, rabbits, and dogs, mitochondrial fractions were prepared in-house using a mitochondrial isolation kit (Cosmo Bio Co., Ltd., Tokyo, Japan) just after the livers were dissected from the animals.

Techniques: Activity Assay