Journal: eLife

Article Title: Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes

doi: 10.7554/eLife.49501

Figure Lengend Snippet: ( A ) Workflow showing DAPI-stained nuclei pre- and post-FACS/filtration that underwent microfluidic partitioning and library preparation in the 10X genomics platform followed by sequencing using an Illumina HiSeq 4000. ( B ) tSNE-plot showing 14 clusters from ~6000 adipocytes derived from iWAT of mice exposed to cold for 24 hr. Each colored dot is an adipocyte assigned to a cluster based on transcriptomic signature. ( C ) Normalized expression values of the top two adipocyte subtype-specific cluster genes from ( B ) plotted as violin plots with clusters as rows and genes as columns. ( D ) tSNE-plot showing cluster-specific expression of selected marker genes from ( C ). ( E ) Normalized expression values of indicated genes in subtype-specific clusters plotted as violin plots with clusters as rows and genes as columns. Black arrow is pointing toward metabolically active Type nine adipocyte cluster and enriched gene.

Article Snippet: Commercial kit , FlowJEM aquapel-treated microfluidic device , FlowJEM , N/A , .

Techniques: Staining, Filtration, Sequencing, Derivative Assay, Expressing, Marker, Metabolic Labelling

Journal: eLife

Article Title: Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes

doi: 10.7554/eLife.49501

Figure Lengend Snippet:

Article Snippet: Commercial kit , FlowJEM aquapel-treated microfluidic device , FlowJEM , N/A , .

Techniques: Isolation, Multiplex Assay, Lysis, Software

Journal: International Journal of Molecular Sciences

Article Title: Characterizing Glomerular Barrier Dysfunction with Patient-Derived Serum in Glomerulus-on-a-Chip Models: Unveiling New Insights into Glomerulonephritis

doi: 10.3390/ijms25105121

Figure Lengend Snippet: Schematic drawing of a three-dimensional microfluidic glomerulus-on-a-chip device to recapitulate the structure and function of the kidney glomerular filtration barrier. ( A ) Illustration (left panel) and an electron micrograph (right panel) of the glomerular filtration barrier (GFB). The GFB comprises podocytes with their foot processes, glomerular basement membrane, and fenestrated endothelial cells. Foot processes are interconnected by slit diaphragms (arrowheads). ( B ) Architecture of the glomerular chip. The chip consists of three cell culture chambers, four microfluidic channels, and two media reservoirs, in which podocytes (CIHP-1) and glomerular endothelial cells (RFP-HGMVECs) are co-cultured on the collagen type I-coated extracellular matrix of the insert to form the GFB. ( C ) Real image of the microfluidic chip in a cell culture dish (100 mm). ( D ) Workflow for evaluating the integrity and reproducibility of GFB and for developing a glomerulonephritis (GN) model into a microfluidic chip. CIHP-1 cells were first seeded on the bottom surface of a cell culture insert. Then, RFP-HGMVECs were seeded on the inside surface of the insert. Patient serum was added to both the chamber and the inside of the insert on day 8 to induce the GN model.

Article Snippet: Viability of CIHP-1 cells on a microfluidic chip was assessed using a cell counting kit-8 (CCK-8, Dojindo, Kumamoto, Japan, #CK-04) according to the manufacturer’s instructions.

Techniques: Filtration, Membrane, Cell Culture