Journal: Circulation Research

Article Title: Inhibition of miR-15 Protects Against Cardiac Ischemic Injury

doi: 10.1161/CIRCRESAHA.111.244442

Figure Lengend Snippet: A, Representative images after TTC staining indicate that although the area at risk (AAR, red and white) is comparable between the different treatment groups, the infarcted area (IA, white) is smaller in the tiny 15b-treated animals (control indicates control oligonucleotide). B, Quantification of cross sections of the infarcted hearts indicate that the AAR is ≈50% of the LV for all 3 treatment groups, whereas administration of 0.5 mg/kg of tiny 15b during reperfusion results in a significant reduction in infarct size compared with either saline or control oligo (*P<0.05 versus saline and control by ANOVA; control indicates control oligonucleotide). C, Real-time PCR analysis on tissue of the ischemic region 24 hours after reperfusion indicates inhibition of miR-15b in response to tiny 15b treatment (*P<0.05 versus saline and control oligonucleotide treated by ANOVA). D, Left ventricular end-diastolic pressure recordings 24 hours after reperfusion reveals an increase with saline treatment and a reduction with tiny 15b treatment (control indicates control oligonucleotide, *P<0.05 versus sham Kruskal-Wallis test). E, Ontology analysis of transcripts upregulated ≥1.5-fold in the ischemic region of hearts 24 hours after reperfusion treated with tiny 15b treatment compared with saline, based on microarray profiling. Negative regulators of apoptosis and cell death are significantly overrepresented. F, Echocardiography shows a reduction in ejection fraction (EF) and increases in LV volumes 2 weeks after infarct, all of which are significantly improved in response to tiny 15b treatment (*P<0.05 versus saline and control by ANOVA for EF and LVESV, versus saline only LVEDV; sham indicates no ischemia/reperfusion; control, control oligo). G, Representative images of Picrosirius red-stained cross sections demonstrate a reduction in collagen content of the left ventricle 2 weeks after reperfusion with tiny 15b treatment. Quantification of fibrosis as a percentage of total left ventricular area reveals a statistically significant reduction in the tiny 15b-treated group (*P<0.05 versus saline-treated by ANOVA). LV indicates left ventricle.

Article Snippet: Microarray for miRNAs and mRNAs Microarray analysis was performed on total RNA, using a service provider (LC Sciences, Houston, TX) as described previously.

Techniques: Staining, Control, Saline, Real-time Polymerase Chain Reaction, Inhibition, Microarray

Journal: Frontiers in Cell and Developmental Biology

Article Title: miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis

doi: 10.3389/fcell.2021.741074

Figure Lengend Snippet: MiR-29b-3p expression in 2D and 3D cultured A549 and MCF7 cells. (A) The morphology of 2D and 3D cultured A549 and MCF7 cells captured under a phase-contrast microscope. (B) Heat map of miRNAs microarray analysis in 2D and 3D cultured A549 cells 0.5 h after 5 Gy X-ray radiation. (C) Fold changes of miRNAs expression that are more than threefold between 2D and 3D cultured cells. (D) Relative miR-29b-3p expression measured by qRT-PCR at indicated time points in 2D and 3D cultured A549 and MCF7 cells after 5 Gy X-ray irradiation. U6 was used as an internal control. Ctrl, unirradiated groups. Significance was determined by unpaired t -test. * P < 0.05; ** P < 0.01.

Article Snippet: Then, the microarray analysis of miRNAs was performed by CapitalBio Corporation following the protocol as previously described ( ).

Techniques: Expressing, Cell Culture, Microscopy, Microarray, Quantitative RT-PCR, Irradiation, Control

Journal: Annals of Medicine

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease

doi: 10.1080/07853890.2025.2530690

Figure Lengend Snippet: Comparison of the expression profiles of miRNAs(A-C) and mRNAs (D-F) between HbH-CS patients and healthy controls. (A) Scatter plot showing the distribution of miRNA expression. (B) Volcano plot showing the differential expression of miRNAs. (C) The clustering heatmap showed differentially expressed miRNAs between patients with HbH-CS patients and healthy controls. (D) Scatter plot showing the distribution of mRNA expression. (E) Volcano plot showing the differential expression of mRNAs. (F) The clustering heatmap showed differentially expressed mRNAs between patients with HbH-CS patients and healthy controls.

Article Snippet: MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5.

Techniques: Comparison, Expressing, Quantitative Proteomics

Journal: Annals of Medicine

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease

doi: 10.1080/07853890.2025.2530690

Figure Lengend Snippet: Bioinformatics analysis. (A) Venn diagram showed stratified operations of miRNAs from the original data and online database. (B) Intersection plot of mRNAs from our previous ArrayStar human mRNA array and miR-223-3p target gene predicted by online database. (C) Prediction plot of miR-223-3p target gene. Yellow circled node, miR-223-3p; blue rectangle type node, mRNA. (D, E) The qRT-PCR was performed to detect the relative expression levels of miR-223-3p (D) and TGFBR3 (E) in the samples from healthy normal subjects and HbH-CS patients. Normal group, n = 17; HbH-CS group, n = 17, mean ± SEM, ** p < 0.01, *** p < 0.001.

Article Snippet: MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5.

Techniques: Quantitative RT-PCR, Expressing

Journal: Oncology Reports

Article Title: Bioinformatic identification of chemoresistance-associated microRNAs in breast cancer based on microarray data

doi: 10.3892/or.2018.6205

Figure Lengend Snippet: Differentially expressed miRNAs (DE-miRNAs) in five cases of drug-resistant (DR) breast cancer tissues and five cases of drug-sensitive (DS) tissues. (A) Data are presented as a heat map. FC, fold change. (B) The chemotherapy drugs used for treating the breast cancer patients.

Article Snippet: The dataset GSE71142, based on the platform of GPL20717 μParafloTM miRNA microarray (LC Sciences, Houston, TX, USA), included five cases of chemoresistant breast cancer tissues and five cases of chemosensitive tissues.

Techniques:

Journal: Oncology Reports

Article Title: Bioinformatic identification of chemoresistance-associated microRNAs in breast cancer based on microarray data

doi: 10.3892/or.2018.6205

Figure Lengend Snippet: KEGG pathway analysis of DE-miRNA target genes. (A) For upregulated miRNAs; and (B) for downregulated miRNAs. The top 10 enriched pathways are presented.

Article Snippet: The dataset GSE71142, based on the platform of GPL20717 μParafloTM miRNA microarray (LC Sciences, Houston, TX, USA), included five cases of chemoresistant breast cancer tissues and five cases of chemosensitive tissues.

Techniques:

Journal: Oncology Reports

Article Title: Bioinformatic identification of chemoresistance-associated microRNAs in breast cancer based on microarray data

doi: 10.3892/or.2018.6205

Figure Lengend Snippet: miRNA-gene regulatory network based on the hub target genes. (A) For upregulated miRNAs; and (B) for downregulated miRNAs.

Article Snippet: The dataset GSE71142, based on the platform of GPL20717 μParafloTM miRNA microarray (LC Sciences, Houston, TX, USA), included five cases of chemoresistant breast cancer tissues and five cases of chemosensitive tissues.

Techniques:

Journal: Oncology Reports

Article Title: Bioinformatic identification of chemoresistance-associated microRNAs in breast cancer based on microarray data

doi: 10.3892/or.2018.6205

Figure Lengend Snippet: Enriched transcription factors by DE-miRNA target genes. (A) For upregulated miRNAs; and (B) for downregulated miRNAs. The top 10 most significant transcription factors are presented.

Article Snippet: The dataset GSE71142, based on the platform of GPL20717 μParafloTM miRNA microarray (LC Sciences, Houston, TX, USA), included five cases of chemoresistant breast cancer tissues and five cases of chemosensitive tissues.

Techniques:

Journal: Oncology Reports

Article Title: Bioinformatic identification of chemoresistance-associated microRNAs in breast cancer based on microarray data

doi: 10.3892/or.2018.6205

Figure Lengend Snippet: Validated miRNA-gene interactions in breast carcinoma. (A) The validated miRNA-gene-human phenotype ontology (HPO) interactions in breast carcinoma were searched from miRWalk2.0. (B) The validated miRNA-gene network was constructed.

Article Snippet: The dataset GSE71142, based on the platform of GPL20717 μParafloTM miRNA microarray (LC Sciences, Houston, TX, USA), included five cases of chemoresistant breast cancer tissues and five cases of chemosensitive tissues.

Techniques: Construct

Journal: PLoS ONE

Article Title: Comprehensive analysis of differentially expressed microRNAs and mRNAs in dorsal root ganglia from streptozotocin-induced diabetic rats

doi: 10.1371/journal.pone.0202696

Figure Lengend Snippet: (A) Schematic overview of the procedure of diabetic rat induction and sample collections; (B) A flow chart of miRNA and mRNA microarray analysis applied in this study.

Article Snippet: The miRNA and mRNA microarray experiments and bioinformatics analysis were conducted by Genminix Informatics Co. Ltd. in Shanghai, China.

Techniques: Microarray

Journal: PLoS ONE

Article Title: Comprehensive analysis of differentially expressed microRNAs and mRNAs in dorsal root ganglia from streptozotocin-induced diabetic rats

doi: 10.1371/journal.pone.0202696

Figure Lengend Snippet: Consistent with the microarray results, rno-miR-1-3p was downregulated and target gene Mgat4a was upregulated in diabetic group compared to control group. Results were presented as mean±SE of three independent experiments (* P <0.05). Samples used for qRT-PCR analysis were independent of microarray samples.

Article Snippet: The miRNA and mRNA microarray experiments and bioinformatics analysis were conducted by Genminix Informatics Co. Ltd. in Shanghai, China.

Techniques: Microarray, Control, Quantitative RT-PCR

Journal: Aging (Albany NY)

Article Title: MicroRNA-506-3p inhibits osteosarcoma cell proliferation and metastasis by suppressing RAB3D expression

doi: 10.18632/aging.101468

Figure Lengend Snippet: Differentially expressed miRNAs in osteosarcomas.

Article Snippet: miRNA microarray analysis was outsourced to RiBio Cor (Ribobio, China).

Techniques:

Journal: Aging (Albany NY)

Article Title: MicroRNA-506-3p inhibits osteosarcoma cell proliferation and metastasis by suppressing RAB3D expression

doi: 10.18632/aging.101468

Figure Lengend Snippet: Identification of miRNAs differentially expressed in osteosarcoma. ( A ) Expression of miRNAs in osteosarcoma tissues and adjacent normal tissues detected using real time PCR. ** P < 0.05 vs. adjacent tissues group. ( B ) Relationship between miR-506-3p and survival in osteosarcoma patients. ( C ) Expression of miR-506-3p in hFOB 1.19, HOS, U2OS, and MG63 cells detected using real time PCR. ** P < 0.05 vs. hFOB 1.19 cells

Article Snippet: miRNA microarray analysis was outsourced to RiBio Cor (Ribobio, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction