Journal: PLOS ONE

Article Title: MicroRNA-26a-5p is a reliable biomarker in the adjuvant setting for pancreatic ductal adenocarcinoma

doi: 10.1371/journal.pone.0310328

Figure Lengend Snippet: (A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

Article Snippet: Messenger RNA (mRNA) microarray analysis was performed by Toray Industries (Tokyo, Japan) using the TORAY 3D‐Gene ® platform.

Techniques: Selection, Microarray, Adjuvant, Isolation

Journal: Circulation. Heart failure

Article Title: Differential microRNA-21 and microRNA-221 upregulation in the biventricular failing heart reveals distinct stress responses of right versus left ventricular fibroblasts

doi: 10.1161/CIRCHEARTFAILURE.119.006426

Figure Lengend Snippet: (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

Article Snippet: miRNA microarray data was analyzed for differential miRNA expression between pre-specified groups (RV-HF vs. RV-Ctrl, LV-HF vs. LV-Ctrl, and RV-HF vs. LV-HF) by two-tailed Student’s t-test using GraphPad Software (Prism 7.0).

Techniques: Expressing, Labeling, Quantitative RT-PCR, Inhibition

Journal: PLoS ONE

Article Title: Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

doi: 10.1371/journal.pone.0077936

Figure Lengend Snippet: Clustering of differentially expressed circulating microRNAs in the whole blood of C567BL/6 mice at 4, 8, and 24 h after cecal ligation and puncture (A) and subcutaneous bacteria injection with 1×10 8 E. coli. or S. aureus (B).

Article Snippet: Mouse genome-wide miRNA microarray analysis and statistical analysis was performed by Phalanx Biotech.

Techniques: Ligation, Bacteria, Injection

Journal: PLoS ONE

Article Title: Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

doi: 10.1371/journal.pone.0077936

Figure Lengend Snippet: Venn diagram showing the common and specific up-regulated miRNAs in the cecal ligation and puncture experiment without and after subcutaneous injection of gram-negative or gram-positive bacteria.

Article Snippet: Mouse genome-wide miRNA microarray analysis and statistical analysis was performed by Phalanx Biotech.

Techniques: Ligation, Injection, Bacteria

Journal: Signal Transduction and Targeted Therapy

Article Title: Natural killer cell-derived exosomal miR-1249-3p attenuates insulin resistance and inflammation in mouse models of type 2 diabetes

doi: 10.1038/s41392-021-00805-y

Figure Lengend Snippet: NK-derived exosomes from lean mice attenuate obesity-induced insulin resistance. a Fasting blood glucose of NCD and HFD mice after group feeding. b – d OGTT ( b ), ITT ( c ), and HOMA-IR index ( d ) of HFD mice and NCD mice ( n = 48) were recorded at 42 days after group feeding. HOMA-IR = Fasting blood glucose value × fasting serum insulin value/22.5. e Transmission electron micrographs of NK-derived exosomes. Scale bar, 100 nm. f NanoSight particle tracking analysis showing the particle size of NK-derived exosomes isolated from NCD and HFD mice. g Western blot assays of exosomal markers TSG101, HSP70, CD63, and CD9. h – k After blank liposomes, NCD-Exos, and HFD-Exos were separately injected into NCD or HFD mice via tail vein, fasting blood glucose ( h ), OGTT ( i ), ITT ( j ), and HOMA-IR ( k ) were assessed in each group. G1: HFD mice treated with blank liposomes ( n = 3); G2: HFD mice treated with HFD-Exos ( n = 3); G3: HFD mice treated with NCD-Exos ( n = 3); G4: NCD mice treated with blank liposomes ( n = 3); G5: NCD mice treated with HFD-Exos ( n = 3); and G6: NCD mice treated with NCD-Exos ( n = 3). l After NCD-Exos labeled with PKH26 were transferred into recipient mice, fluorescence images of the liver, islets, SATs, VATs, and skeletal muscles were observed by in vitro imaging system. Scale bar, 5 mm. m The weight of VATs from HFD mice treated with NCD-Exos, HFD-Exos, or blank liposomes. n Liver triglyceride content of HFD mice treated with NCD-Exos, HFD-Exos, or blank liposomes. o , p ELISA assays of IL-6, IL-1β, and TNF-α expression in VATs and livers of HFD mice treated with NCD-Exos, HFD-Exos, or blank liposomes. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t-test was used to analyze the data. (* P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: Exosomal miRNA microarray analysis was performed at CapitalBio Technology, Inc. (Beijing, China) using Agilent Mouse miRNA 8 × 60K V21.0 Microarray (Agilent Technologies, California, USA).

Techniques: Derivative Assay, Transmission Assay, Isolation, Western Blot, Liposomes, Injection, Labeling, Fluorescence, Muscles, In Vitro, Imaging, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Signal Transduction and Targeted Therapy

Article Title: Natural killer cell-derived exosomal miR-1249-3p attenuates insulin resistance and inflammation in mouse models of type 2 diabetes

doi: 10.1038/s41392-021-00805-y

Figure Lengend Snippet: NK-derived exosomal miR-1249-3p mediates cellular insulin sensitivity and inflammation. a Microarray analysis of significantly expressed exosomal miRNAs between NCD-Exos and HFD-Exos was presented in a heatmap. b qRT-PCR assay of miR-1249-3p expression in splenic NK cells, NK-derived exosomes, and circulating exosomes from NCD or HFD mice. c NK cells transfected with a Cy3-labeled miR-1249-3p mimic were co-cultured with 3T3-L1 adipocytes or AML12 cells in a Transwell TM plate (membrane pore = 0.4 mm) plate. Scale bar, 100 μm. d – g After transfecting with miR-1249-3p mimic, miR-NC, inhibitor, and inh-NC, the glucose uptake content of 3T3-L1 adipocytes ( d ) and glucose production content of AML12 cells ( f ) were measured, and the concentrations of IL-6, TNF-α, and IL-1β secreted by 3T3-L1 adipocytes ( e ) and AML12 cells ( g ) were detected by ELISA. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t -test was used to analyze the data. (* P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: Exosomal miRNA microarray analysis was performed at CapitalBio Technology, Inc. (Beijing, China) using Agilent Mouse miRNA 8 × 60K V21.0 Microarray (Agilent Technologies, California, USA).

Techniques: Derivative Assay, Microarray, Quantitative RT-PCR, Expressing, Transfection, Labeling, Cell Culture, Membrane, Enzyme-linked Immunosorbent Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: Natural killer cell-derived exosomal miR-1249-3p attenuates insulin resistance and inflammation in mouse models of type 2 diabetes

doi: 10.1038/s41392-021-00805-y

Figure Lengend Snippet: Exosomal miR-1249-3p directly targets SKOR1 to mediate insulin sensitivity. a The potential targets of miR-1249-3p were predicted by integrating the results of two databases (TargetScan and miRDB). b Western blot analysis of SKOR1 in 3T3-L1 adipocytes and AML12 cells with the indicated treatments. c The wild-type and a mutated type of binding site between miR-1249-3p and SKOR1. d Relative luciferase activity of AML12 cells in the presence of indicated treatments. e Western blot analysis of SKOR1 expression in the VATs and livers of HFD mice after NCD-Exos or HFD-Exos treatment. f – i The effect of sh-SKOR1 on glucose uptake capacity in 3T3-L1 adipocytes ( f ), glucose production capacity in AML12 cells ( g ), and expression levels of IL-6, TNF-α, and IL-1β ( h , i ). j – q Glucose uptake content of 3T3-L1 adipocytes ( j ) and glucose production content of AML12 cells ( n ) with the indicated treatments were measured, as well as the expression levels of IL-6, TNF-α, and IL-1β ( k – m and o – q ). Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t -test was used to analyze the data. (* P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: Exosomal miRNA microarray analysis was performed at CapitalBio Technology, Inc. (Beijing, China) using Agilent Mouse miRNA 8 × 60K V21.0 Microarray (Agilent Technologies, California, USA).

Techniques: Western Blot, Binding Assay, Luciferase, Activity Assay, Expressing

Journal: Signal Transduction and Targeted Therapy

Article Title: Natural killer cell-derived exosomal miR-1249-3p attenuates insulin resistance and inflammation in mouse models of type 2 diabetes

doi: 10.1038/s41392-021-00805-y

Figure Lengend Snippet: MiR-1249-3p relieves insulin resistance and inflammation via the SKOR1-SMAD6-TLR4-NF-κB axis. a – c After transfection with a miR-1249-3p mimic, miR-NC, or specific siRNA to SKOR1 or SMAD6, qRT-PCR analysis of miR-1249-3p ( a ), SKOR1 ( b ) and SMAD6 ( c ) expression in 3T3-L1 adipocytes cells with the indicated treatments was performed. d , g The expression of p-p65 and p65 in 3T3-L1 adipocytes cells with the indicated treatments was performed by western blot. e , f , h IL-1β, IL-6, and TNF-α expression in 3T3-L1 adipocytes cells subjected to the indicated treatments was assessed by ELISA. i The signaling pathway through which NK-derived exosomal miR-1249-3p regulates insulin resistance in type 2 diabetes mice. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t -test was used to analyze the data. (* P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: Exosomal miRNA microarray analysis was performed at CapitalBio Technology, Inc. (Beijing, China) using Agilent Mouse miRNA 8 × 60K V21.0 Microarray (Agilent Technologies, California, USA).

Techniques: Transfection, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay