Journal: Viruses

Article Title: Distinguishing Incubation and Acute Disease Stages of Mild-to-Moderate COVID-19

doi: 10.3390/v14020203

Figure Lengend Snippet: Kinetics of plasmablast proportions and SARS-CoV-2 specific immunoglobulin enrichment delineated disease stages during mild-to-moderate COVID-19. ( A ) Representative pseudocolor plots of CD38 and CD27 expression data of CD19 + CD45RA + B lymphocytes showed the disease stage-dependent increase of plasmablast proportions (rectangular gate). ( B ) Top left: Quantitative data of plasmablast abundances at different disease stage. Top right, bottom left and bottom right: Semi-quantitative data of SARS-CoV-2 specific IgM, IgG and IgA titers in plasma samples. The data for anti-RBD, -S1, -S2 and –N were combined for IgM and IgG, respectively.* p < 0.05; ** p < 0.01; *** p < 0.001; Tukey-Kramer test for plasmablasts, Kruskal-Wallis test with Dunn’s correction for multiple comparisons for Antibody isotypes. MFI: Median fluorescence intensity. A 450 : Absorbance at 450 nm.

Article Snippet: For the semi-quantitative analyses of IgM and IgG specific for the nucleocapsid (N) or the spike protein subunits S1, S2 and receptor binding domain (RBD), we used the respective MILLIPLEX SARS-CoV-2 antigen panel kits (Merck) to the manufacturer’s instructions.

Techniques: Expressing, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Obesity and Dyslipidemia Synergistically Exacerbate Psoriatic Skin Inflammation

doi: 10.3390/ijms23084312

Figure Lengend Snippet: Measurement of multiple adipokine levels in the mouse serum: ( a – d ) Serum levels of leptin, resistin, IL-6, and MCP-1. Serum samples were collected from wild-type and Apoe −/− mice fed with a normal diet or a high-fat diet and treated with imiquimod cream (25 mg/mouse) or Vaseline (control) ( n = 6 per group). Serum adipokine levels were measured by multiplex assay. ( e ) Serum IL-17A concentrations determined by ELISA. Values are presented as the mean ± standard deviation; * p < 0.05, † p < 0.05, compared to Vaseline-treated mice. IMQ, imiquimod; WT, wild-type; ND, normal diet; HFD, high-fat diet.

Article Snippet: Adipokine profiles in mouse sera were determined using a Milliplex mouse adipokine magnetic bead panel (Merck Millipore, Billerica, MA, USA), as reported previously [ ].

Techniques: Cream, Control, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation