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Recombinant mAb against mouse IL-2 (clone S4B6), D265A effectorless. For in vitro use
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Cellular Technology Ltd
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Becton Dickinson
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Bio-Techne corporation
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GenScript corporation
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Strathmann Biotec AG
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AbMax Inc
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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Alpaca ( Vicugna pacos ), the first nonprimate species with a phosphoantigen-reactive Vγ9Vδ2 T cell subset
doi: 10.1073/pnas.1909474117
Figure Lengend Snippet: BTN3-dependent PAg reactivity of transduced alpaca Vγ9Vδ2 TCRs. (A) Murine responder cells (53/4) expressing vpTCR(Jδ2), vpTCR(Jδ4), or a human Vγ9Vδ2 TCR (huTCR) were cocultured with 293T cell lines (WT), 293T BTN3KO, or BTN3KO vpBTN3 (phNGFR mCherry) in the presence of titrated HMBPP doses. Supernatants were tested for mIL-2 after 22 h. (B) In addition, WTH-5 (1 µg/mL) or mIgG2b,κ isotype were added to cultures of TCR transductants with wild-type or BTN3KO vpBTN3 in the presence of 10 µM HMBPP. Duplicates were measured in three experiments and mean + SD are shown. Statistical analysis is shown in SI Appendix, Table S6 for A and a two-way ANOVA (B) was carried out with a Bonferroni post hoc test (ns, not significant: P > 0.05, ****P < 0.0001).
Article Snippet: The activation of TCR transductants was measured via
Techniques: Expressing
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Alpaca ( Vicugna pacos ), the first nonprimate species with a phosphoantigen-reactive Vγ9Vδ2 T cell subset
doi: 10.1073/pnas.1909474117
Figure Lengend Snippet: The alpaca B30.2 domain can sense PAgs. (A) A number of 1 × 104 293T BTN3KO or BTN3A1KO cells transduced with huBTN3A1, vpBTN3, hu/vpBTN3, or vp/huBTN3 (all in phNGFR mCherry) were plated overnight. Next, 5 × 104 Murine responder cells [53/4 huTCR or vpTCR(Jδ2)] were added as well as a dilution of HMBPP. Medium was used as a control, and the experiment was carried out in duplicates. Supernatants were tested for mIL-2 after 22 h with mIL-2 ELISA. Means of three independent experiments are shown with SDs. Statistical analysis is summarized in SI Appendix, Table S7. (B) Graphical summary of A with schematic representation of wild-type human (hu) and alpaca BTN3 (vp) and constructed chimeric molecules with an exchange of intracellular domains (hu/vp and vp/hu). The mIL-2 response of responder cells is indicated as high (+++), intermediate (++), low (+), or negative (−).
Article Snippet: The activation of TCR transductants was measured via
Techniques: Transduction, Enzyme-linked Immunosorbent Assay, Construct