midi macs separator Search Results


midi macs ls separation columns  (Miltenyi Biotec)
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Miltenyi Biotec midi macs ls separation columns
Midi Macs Ls Separation Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi macs separator  (Miltenyi Biotec)
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Miltenyi Biotec midi macs separator
Midi Macs Separator, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi macs1 (magnetic activated cell sorting) ls1 separation columns  (Miltenyi Biotec)
90
Miltenyi Biotec midi macs1 (magnetic activated cell sorting) ls1 separation columns
Midi Macs1 (Magnetic Activated Cell Sorting) Ls1 Separation Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi macs cd34 progenitor cell isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec midi macs cd34 progenitor cell isolation kit
Midi Macs Cd34 Progenitor Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34 microbead kit  (Miltenyi Biotec)
99
Miltenyi Biotec cd34 microbead kit
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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midi macs cd4 t cells magnetic isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec midi macs cd4 t cells magnetic isolation kit
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Macs Cd4 T Cells Magnetic Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd90 2 microbeads  (Miltenyi Biotec)
97
Miltenyi Biotec cd90 2 microbeads
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Cd90 2 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nextseq 500 550 v2 kits  (Illumina Inc)
96
Illumina Inc nextseq 500 550 v2 kits
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Nextseq 500 550 V2 Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi mac separation column  (Miltenyi Biotec)
97
Miltenyi Biotec midi mac separation column
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Mac Separation Column, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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nitrocellulose membranes bio rad laboratories cat  (Bio-Rad)
96
Bio-Rad nitrocellulose membranes bio rad laboratories cat
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Nitrocellulose Membranes Bio Rad Laboratories Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi macs separator unit according to the manufacturer's instructions  (Miltenyi Biotec)
90
Miltenyi Biotec midi macs separator unit according to the manufacturer's instructions
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Macs Separator Unit According To The Manufacturer's Instructions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midi macs separator unit according to the manufacturer's instructions - by Bioz Stars, 2026-03
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( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of CD34+ cells transduced with shTP53 is significantly lower than in CD34+ cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Journal: Oncotarget

Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model

doi: 10.18632/oncotarget.25581

Figure Lengend Snippet: ( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of CD34+ cells transduced with shTP53 is significantly lower than in CD34+ cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Techniques: Transduction, Expressing, Comparison

( A ) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. ( B ) CFU results of knockdown of cells transduced with wt TP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wt TP53. ( C ) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Journal: Oncotarget

Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model

doi: 10.18632/oncotarget.25581

Figure Lengend Snippet: ( A ) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. ( B ) CFU results of knockdown of cells transduced with wt TP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wt TP53. ( C ) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Techniques: Knockdown, shRNA, Control, Transduction, Irradiation, Expressing