Journal: Advanced healthcare materials

Article Title: Cell and Material-Specific Phage Display Peptides Increase iPS-MSC Mediated Bone and Vasculature Formation In Vivo

doi: 10.1002/adhm.201801356

Figure Lengend Snippet: Morphology and distribution of bone-like mineral precipitated into the pores of PLGA scaffolds. Representative MicroCT image of mineralized scaffold rendered in MicroView®(A). Representative images of mineral distribution in volumetric shells progressing from the outermost to innermost regions of the scaffold (B-F). Fraction of mineral volume from the outermost to the innermost volumetric shell of the scaffolds (G). * indicates significant difference from sections 1 and 2 (p<0.001, n = 10).

Article Snippet: Reconstructed images were rendered in (MicroView, Parallax Innovations Inc) using the isosurface tool at a threshold of 1000 with the smoothing filter and a surface quality factor of 0.51.

Techniques:

Journal: Advanced healthcare materials

Article Title: Cell and Material-Specific Phage Display Peptides Increase iPS-MSC Mediated Bone and Vasculature Formation In Vivo

doi: 10.1002/adhm.201801356

Figure Lengend Snippet: Bone volume fractions of ectopically regenerated bone 8 weeks post iPS-MSC transplantation (n=6). Representative rendered MicroView® images of bone regenerated with A) acellular bone-like mineral (BLM), acellular BLM containing DPI-VTK, BLM with iPS-MSCs, BLM containing VTK with iPS-MSCs, BLM containing DPI-VTK with iPS-MSCs, P15 coated BLM constructs with iPS-MSCs (scale bar=1mm). B) Bone volume fractions from MicroCT analysis indicate significantly greater bone formation in scaffolds with DPI-VTK and P15 compared to VTK, BLM, DPI-VTK acellular and BLM acellular controls. * indicates a significant difference from BLM and acellular BLM (p <0.001). ** indicates a significant difference from VTK (p < 0.001), BLM (p < 0.001), acellular DPI-VTK (p < 0.001) and acellular BLM controls (p < 0.001).

Article Snippet: Reconstructed images were rendered in (MicroView, Parallax Innovations Inc) using the isosurface tool at a threshold of 1000 with the smoothing filter and a surface quality factor of 0.51.

Techniques: Transplantation Assay, Construct

Journal: JCI Insight

Article Title: Intravascular hemolysis activates complement via cell-free heme and heme-loaded microvesicles

doi: 10.1172/jci.insight.96910

Figure Lengend Snippet: (A) NHS was incubated with 50 μM hemin, 12.5 μM Hb (1 molecule of Hb contains 4 heme molecules) diluted in TBS. After a 30-minute incubation time at 37°C, the level of released Ba was measured by ELISA. *P < 0.005, Kruskal-Wallis with Dunn’s test for multiple pairwise comparisons. (B) HUVECs were treated with increasing concentrations of hemin or diluted in FCS-free culture medium (M199) for 30 minutes at 37°C. After removing the supernatant, HUVECs were exposed to NHS (33% final concentration diluted in the same medium) for 30 minutes at 37°C. Cells were detached and stained for C3 deposition by flow cytometry. (C–F) HUVECs were treated with increased concentrations of hemin with or without 5 μM of Hx for 30 minutes at 37°C. After removing the supernatant, HUVECs were exposed to NHS (33% final concentration diluted in M199 FCS-free) for 30 minutes at 37°C. Cells were detached and stained for C3 deposition (C and D) or C5b-9 formation (E and F) by flow cytometry. (C and E) Representative flow cytometry histograms. (D and F) Quantitative analyses. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001, 2-way ANOVA with Sidak’s test for multiple comparisons. Values are shown as box plots with median and minimum/maximum points.

Article Snippet: Complement ELISA kits MicroView Ba and sC5b-9 were from Quidel.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Concentration Assay, Staining, Flow Cytometry

Journal: JCI Insight

Article Title: Intravascular hemolysis activates complement via cell-free heme and heme-loaded microvesicles

doi: 10.1172/jci.insight.96910

Figure Lengend Snippet: (A and B) NHS was incubated with 1,000 MV/μl RBC MVs from HDs (n = 12) or SCD patients (n = 12) diluted in TBS. After 30 minutes at 37°C, the levels of released Ba (A) and sC5b-9 (B) were measured by ELISA. **P < 0.005, Mann-Whitney test. (C) Linear correlation between measured Ba and sC5b-9 levels with RBC MPs from SCD (n = 12) in tested samples. R2 = 0.4811; P = 0.0085. (D and E) NHS was incubated with 1,000 MV/μl MVs from SCD patients (n = 12) diluted in TBS with or without 25 μM Hx. After 30 minutes at 37°C, the levels of released Ba (D) and sC5b-9 (E) were measured by ELISA. *P < 0.05, ***P < 0.001, Wilcoxon test after a Shapiro-Wilk test for normality. (F and G) HUVECs were treated with 1,000 MV/μl RBC MPs from HDs (n = 4) or SCD patient (n = 7) diluted in M199 medium for 30 minutes at 37°C. Without removing the supernatant, HUVECs were exposed to NHS (final concentration at 33% diluted in M199 FCS-free) for 30 minutes at 37°C. Cells were detached and stained for C3 deposition by flow cytometry. (F) Flow cytometry histograms. (G) Quantitative analyses. *P < 0.05, Mann-Whitney test. (H) HUVECs were treated with 1,000 MV/μl RBC MPs from 1 SCD patient with or without 5 μM Hx. As above, NHS was added and cells were stained for C3 deposition by flow cytometry. Values are shown as box plots with median and minimum/maximum points.

Article Snippet: Complement ELISA kits MicroView Ba and sC5b-9 were from Quidel.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Concentration Assay, Staining, Flow Cytometry