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RADWAG Balances & Scales
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METTLER TOLEDO
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Image Search Results
Journal: Hypertension
Article Title: Central Pressor Actions of Aminopeptidase-Resistant Angiotensin II Analogs
doi: 10.1161/hypertensionaha.107.087130
Figure Lengend Snippet: Figure 7. HPLC analysis of the fate of 125/127I-labeled Ang II and N-Methyl-L-Asp1 Ang II after ICV injection. Rats were euthanized at the time of onset of the dipsogenic response and the identity of the 125I-labeled material extracted from the brains was deter- mined on a reverse phase C18 column. A, Chromatogram of an extract of a rat brain (A-7), demonstrative of 3 rats injected with 125/127I-Ang II. B, Chromatogram of an extract of a rat brain (A-9), demonstrative of 4 rats injected with 125/127I-N-Methyl-L-Asp1 Ang II. NMLA is N-Methyl-L-Asp1 Ang II. Fragments of N-Methyl- L-Asp1 Ang II were not available for radiolabeling, so the large peak corresponding to Ang2–5 may not be Ang2–5 and could represent N-Methyl-L-Asp1 Ang1–7. Please see data supplement for methodological details.
Article Snippet: The filtrate was analyzed by HPLC using a 250 x 4.6mm
Techniques: Labeling, Injection, Radioactivity