Journal: NPJ Biofilms and Microbiomes

Article Title: Bacterial biofilm functionalization through Bap amyloid engineering

doi: 10.1038/s41522-022-00324-w

Figure Lengend Snippet: Time-lapse fluorescence microscopy was performed to monitor mCherry expression at single-cell level in S. aureus Bap-mCherry. In order to visually compare image patterns, the histogram range of each image was modified accordingly. Bacteria were grown at 37 °C in CellAsic microfluidic plates with a continuous flow of LB for 330 min. Next, bacteria were challenged with LB (neutral pH) a or with acidified LB b for 90 min. Arrows show the point in which the media was changed. Images were taken in 30 min intervals. The mCherry fluorescence and the differential interference contrast (DIC) images are shown. Right panels show the magnification of images taken 30 min before and 60 min after media challenge. Scale bar of panels represents 5 μm.

Article Snippet: S. aureus Bap-Spy was grown in CellAsic microfluidic plates at 37 °C with a continuous flow for 300 min. Then, rCatcher-GFP (left column) and rGFP (right column) were added and the flow was maintained during 2 h with the recombinant proteins.

Techniques: Fluorescence, Microscopy, Expressing, Modification

Journal: NPJ Biofilms and Microbiomes

Article Title: Bacterial biofilm functionalization through Bap amyloid engineering

doi: 10.1038/s41522-022-00324-w

Figure Lengend Snippet: a Schematic diagram of the experiment. S. aureus Bap-Spy was grown in CellAsic microfluidic plates at 37 °C with a continuous flow for 300 min. Then, rCatcher-GFP (left column) and rGFP (right column) were added and the flow was maintained during 2 h with the recombinant proteins. Next, channels were washed with PBS during 90 min to remove the background fluorescence. b Images of GFP fluorescence and the differential interference contrast (DIC) at different times are shown. S. aureus Bap-Spy was grown in microfluidic plates and were incubated with rCatcher-GFP (left column) and rGFP (right column). Images were recorded every 30 min in separate fields to avoid photobleaching. Scale bar of panels represents 5 μm.

Article Snippet: S. aureus Bap-Spy was grown in CellAsic microfluidic plates at 37 °C with a continuous flow for 300 min. Then, rCatcher-GFP (left column) and rGFP (right column) were added and the flow was maintained during 2 h with the recombinant proteins.

Techniques: Recombinant, Fluorescence, Incubation

Journal: Cell systems

Article Title: tRNA Methylation Is a Global Determinant of Bacterial Multi-Drug Resistance

doi: 10.1016/j.cels.2019.03.008

Figure Lengend Snippet: Key Resources Table

Article Snippet: Microfluidic perfusion plates , CellASIC , Cat. #B04a.

Techniques: Virus, Recombinant, Plasmid Preparation, Purification, Software, Mass Spectrometry, Targeted Proteomics, Membrane, Imaging, Fluorescence, Microscopy

Journal: Journal of Dental Research

Article Title: Biochemical Indicators of Implantation Success of Tissue-Engineered Oral Mucosa

doi: 10.1177/0022034514554225

Figure Lengend Snippet: Box plots of the distribution of interleukin-8 (IL-8), human β-defensin 1 (hBD-1), and vascular endothelial growth factor (VEGF) (A), and tissue inhibitor of metalloproteinase 1 and 2 (TIMP-1 and TIMP-2) (B) enzyme-linked immunosorbent assay data by control and thermally stressed groups of ex vivo–produced oral mucosa equivalents. The P values from the test for differences in means were 0.04 for IL-8, 0.03 for hBD-1, 0.07 for VEGF, 0.15 for TIMP-1, and 0.02 for TIMP-2. Statistical analysis strategy is detailed in the Materials and Methods section.

Article Snippet: For microchannel ELISA, OptimserTM microfluidic ELISA plate assembly, buffers, and reagents were from Siloam Biosciences (Cincinnati, OH).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Ex Vivo, Produced