Journal: Cell

Article Title: A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity

doi: 10.1016/j.cell.2019.11.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Microfluidic chips for drop-seq , FlowJEM , Custom order.

Techniques: Recombinant, Red Blood Cell Lysis, Modification, Saline, Activation Assay, Blocking Assay, Plasmid Preparation, Reverse Transcription, Sterility, Injection, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Library Quantification, RNA Sequencing, Sequencing, Software, Adhesive, Cell Counting

Journal: bioRxiv

Article Title: Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

doi: 10.1101/2021.01.10.426076

Figure Lengend Snippet: scRNA-seq of penaeid shrimp M. japonicus hemocytes. (A) Schematics of the microfluidics-based scRNA-seq, preparation of the de novo assembled gene list, in silico analysis workflow and morphology-based cell classification. (B) UMAP (uniform manifold approximation and projection) plot of SCTransform batch corrected and integrated of hemocytes from three shrimps ( n = 2,704).

Article Snippet: To validate the Drop-seq results on hemocytes, populations of hemocytes in the forward scatter (FSC) and side scatter (SSC) two-dimensional space were sorted using a microfluidic cell sorter (On-chip sort, On-chip Biotechnologies Co., Ltd.) from four shrimp individuals (Fig. S7).

Techniques: In Silico

Journal: Biological Psychiatry Global Open Science

Article Title: Single-Cell RNA Sequencing and Its Applications in the Study of Psychiatric Disorders

doi: 10.1016/j.bpsgos.2022.03.013

Figure Lengend Snippet: Microfluidics device for single-cell and barcoded bead encapsulation in oil emulsion.

Article Snippet: Two years after Drop-seq came out, 10X Genomics launched a very similar method that uses a microfluidic device to encapsulate single cells together with a barcoded bead in a droplet, inside which both capture and reverse transcription occur ( ).

Techniques: Encapsulation, Emulsion