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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for the development, assembly, and testing of a synthetic skin microbial community
doi: 10.1016/j.xpro.2025.103714
Figure Lengend Snippet: Applying SkinCom to a mouse model for reproducible, multiomic study (A) SkinCom applied to agar patches and fastened securely to prepped mice backs using transparent film dressing. Such fastening ensures the applied community is not removed by the mice for the duration of the experiment. (B) Mice backs are assessed for any lesions prior to swabbing, indicative of inflammation from applied Skincom. (C) Swab tips are snapped off sterilely for CFU counting, DNA and RNA extractions (using bead beating approaches for lysing following ZymoBIOMICS instructions) for metagenomics and metatranscriptomics.
Article Snippet: Snap off the tips of the remaining two swabs for DNA and
Techniques:
Journal: STAR Protocols
Article Title: Protocol for the development, assembly, and testing of a synthetic skin microbial community
doi: 10.1016/j.xpro.2025.103714
Figure Lengend Snippet:
Article Snippet: Snap off the tips of the remaining two swabs for DNA and
Techniques: Recombinant, In Vitro, In Vivo, Software, Cream, Sterility
Journal: Microorganisms
Article Title: Comparative Evaluation of an Easy Laboratory Method for the Concentration of Oocysts and Commercial DNA Isolation Kits for the Molecular Detection of Cyclospora cayetanensis in Silt Loam Soil Samples
doi: 10.3390/microorganisms10071431
Figure Lengend Snippet: Characteristics of three commercial DNA isolation kits for soil and a concentration by flotation protocol following by DNA isolation as in BAM Chapter 19b, used in this study to extract genomic DNA from soil samples inoculated with 100 oocysts of Cyclospora cayetanensis .
Article Snippet: The sensitivity of the flotation procedure was compared to other common commercial DNA isolation kits (Fast DNA TM 50 mL SPIN kit for soil (MP Biomedicals),
Techniques: DNA Extraction, Concentration Assay, Incubation
Journal: Microorganisms
Article Title: Comparative Evaluation of an Easy Laboratory Method for the Concentration of Oocysts and Commercial DNA Isolation Kits for the Molecular Detection of Cyclospora cayetanensis in Silt Loam Soil Samples
doi: 10.3390/microorganisms10071431
Figure Lengend Snippet: Comparison of average C T values for C. cayetanensis detection in soil samples (10 g) seeded with 100 oocysts after DNA extraction by different methods (Flotation and BAM Chapter 19b Method 1; Fast DNA TM 50 mL SPIN kit for soil (MP Biomedicals) Method 2, Quick-DNA TM Fecal/Soil Microbe Midiprep kit (Zymo Research), Method 3, and DNeasy ® PowerMax ® Soil Kit (Qiagen) using bead-beating, Method 4b). Different letters on top of methods indicate statistically significant differences ( p < 0.05).
Article Snippet: The sensitivity of the flotation procedure was compared to other common commercial DNA isolation kits (Fast DNA TM 50 mL SPIN kit for soil (MP Biomedicals),
Techniques: Comparison, DNA Extraction
Journal: Ecology and Evolution
Article Title: Microbial DNA extraction method for avian feces and preen oil from diverse species
doi: 10.1002/ece3.70220
Figure Lengend Snippet: Cost comparison of three commercial fecal DNA extraction kits with our extraction method.
Article Snippet:
Techniques: Comparison, DNA Extraction, Extraction