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Image Search Results
Journal: OncoTargets and therapy
Article Title: Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer
doi: 10.2147/OTT.S228678
Figure Lengend Snippet: Heat map of the 103 DEGs identified in the integrated microarray analysis. Each column represents one dataset, and each row represents one gene. The number in each rectangle is the log 2 FC value. The color gradient from blue to red represents the progression from down- to upregulation. Abbreviations: DEGs, differentially expressed genes; FC, fold change.
Article Snippet:
Techniques: Microarray
Journal: Journal of Allergy
Article Title: How Can Microarrays Unlock Asthma?
doi: 10.1155/2012/241314
Figure Lengend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
Article Snippet: In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an
Techniques: Expressing, Derivative Assay, Labeling, Reverse Transcription, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing
Journal: Journal of Allergy
Article Title: How Can Microarrays Unlock Asthma?
doi: 10.1155/2012/241314
Figure Lengend Snippet: The GEO accession number for microarray studies conducted on asthma.
Article Snippet: In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an
Techniques: Microarray, Gene Expression, Clone Assay, Expressing, Virus, Infection, Functional Assay, Sequencing, Synthesized, Comparison