Journal: EBioMedicine

Article Title: The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease

doi: 10.1016/j.ebiom.2019.03.054

Figure Lengend Snippet: Nuclear translocation of Notch1 and NFκB active subunits was abrogated in LPS treated BMDM from DNER deficient mice. (a) Representative Western blot of NICD1 protein levels in cytoplasmic and nuclear extracts of samples from WT and DNER deficient BMDM treated with LPS (1μg/ml) for 24 h and untreated (2 independent experiments, 2/3 biological replicates). (b) mRNA abundance of Notch receptors ( Notch1, Notch2 ) and ligands ( Delta4, Jag1 ) in the samples from (a). Two-way ANOVA, Tukey's multiple comparisons test, * P < 0.05, *** P < 0.001. Data shown mean values ± SD. (c) Cytoplasmic and nuclear RelB, c-Rel, p105, p50 and p65 from samples described in (a). (d) Representative Western blots of phosphorylated (p)-IKKα/β, IKKα and IKKβ from 15 min LPS (1μg/ml) treated WT and DNER deficient BMDM. 2 independent experiments and 2/3 biological replicates, n = 3 (e) Enrichment plot of NFkB signalling (GO:0051059) following GSEA analysis of the microarray data from WT M1 vs DNER deficient M1 BMDM.

Article Snippet: Primary antibodies: NICD1 (1:500, ab8925, Abcam), RelB (1:200, sc-226, Santa Cruz), cRel (1:100, sc-6955, Santa Cruz), p105/50 (1:100, sc-1190, Santa Cruz), p65 (1:500, sc-372, Santa Cruz), pIKKα/β (1:1000 #2697, Cell Signalling), IKKα (1:1000 #2682S, Cell Signalling), IKKβ (1:1000 clone D30C6, #8943, Cell Signalling).

Techniques: Translocation Assay, Western Blot, Microarray

Journal: Cell

Article Title: Gene Regulatory Programs Conferring Phenotypic Identities to Human NK Cells

doi: 10.1016/j.cell.2018.11.045

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DX5 microbeads , Miltenyi , Cat#130–052-501.

Techniques: Microscopy, Recombinant, DNA Library Preparation, Isolation, Microarray, Knock-Out, Expressing, Software