Journal: PLOS Computational Biology

Article Title: Mechanical network equivalence between the katydid and mammalian inner ears

doi: 10.1371/journal.pcbi.1012641

Figure Lengend Snippet: (a) An actual image of the bush-cricket Mimetica sp.; (b) The pinna covering the tympanal membranes of Mimetica sp., located at the femoro-tibial joint, obtained from 3D-volume renders of synchrotron micro-CT scans; (c) The hearing organ ( crista acustica ) located on the dorsal wall tapering in width, obtained from 3D-volume renders of synchrotron micro-CT scans; (d) A zoomed-in image of the crista acustica components, obtained from 3D-volume renders of synchrotron micro-CT scans; (e) A drawing of the human and katydid middle and inner ears for comparison.

Article Snippet: The geometry which was used as the solution domain of the mathematical models was the precise geometry of the species Mimetica sp., obtained through synchrotron-based Micro-Computed Tomography ( μ –CT) Scanning and 3D reconstruction.

Techniques: Micro-CT, Comparison

Journal: PLOS Computational Biology

Article Title: Mechanical network equivalence between the katydid and mammalian inner ears

doi: 10.1371/journal.pcbi.1012641

Figure Lengend Snippet: The image of the Mimetica sp. inner ear geometry forming the solution domain of the mathematical model, as obtained by synchrotron-based μ − CT scans and 3D-reconstruction.

Article Snippet: The geometry which was used as the solution domain of the mathematical models was the precise geometry of the species Mimetica sp., obtained through synchrotron-based Micro-Computed Tomography ( μ –CT) Scanning and 3D reconstruction.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: A Computed Tomographic (CT) and Pathological Study of Equine Cheek Teeth Infundibulae Extracted From Asymptomatic Horses. Part 1: Prevalence, Type and Location of Infundibular Lesions on CT Imaging

doi: 10.3389/fvets.2019.00124

Figure Lengend Snippet: (A) Longitudinal microCT image of a maxillary cheek tooth with Grade 2 caries of its rostral infundibulum that has an apical diverticulum, as evidenced by loss of definition of the apical infundibular enamel, with an irregular and hypointense outline of its site visible (arrow). Some of surrounding dentine seems hypointense indicating it also may have changes, (possible Grade 3 caries). The subocclusal defect that connected with and allowed oral bacteria to access this site is not shown in this imaging plane. x1. (B) Longitudinal microCT image of a maxillary cheek tooth with grade 2 caries (cement and enamel involvement) of its rostral infundibulum (following central linear defect and/or apical cemental hypoplasia). The apical dentine appears normal. The occlusal defect allowing extension of occlusal caries to this apical site is visible (yellow arrow). x 1.

Article Snippet: Micro computed tomographic (MicroCT) scanning (XTreme CT, Scanco Medical AG, Bruttisellen, Switzerland) at an isotropic spatial resolution of 82 micrometers was additionally performed on 8 of the above cheek teeth, including Triadan 08 ( n = 1), 09 ( n = 6), 10 ( n = 1) from age groups <5 y.o. ( n = 2); 5–10 y.o. ( n = 2); 10–15 y.o. ( n = 1); 15–20 y.o. ( n = 2); >20 y.o. ( n = 1).

Techniques: Bacteria, Imaging

Journal: Frontiers in Veterinary Science

Article Title: A Computed Tomographic (CT) and Pathological Study of Equine Cheek Teeth Infundibulae Extracted From Asymptomatic Horses. Part 1: Prevalence, Type and Location of Infundibular Lesions on CT Imaging

doi: 10.3389/fvets.2019.00124

Figure Lengend Snippet: Transverse microCT image of infundibulum with combined central linear defect and apical cemental hypoplasia. Note the close proximity of the infundibulum and pulp horn at a site of thin infundibular enamel and adjacent thin dentine (green line). x 1.

Article Snippet: Micro computed tomographic (MicroCT) scanning (XTreme CT, Scanco Medical AG, Bruttisellen, Switzerland) at an isotropic spatial resolution of 82 micrometers was additionally performed on 8 of the above cheek teeth, including Triadan 08 ( n = 1), 09 ( n = 6), 10 ( n = 1) from age groups <5 y.o. ( n = 2); 5–10 y.o. ( n = 2); 10–15 y.o. ( n = 1); 15–20 y.o. ( n = 2); >20 y.o. ( n = 1).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: A Computed Tomographic (CT) and Pathological Study of Equine Cheek Teeth Infundibulae Extracted From Asymptomatic Horses. Part 1: Prevalence, Type and Location of Infundibular Lesions on CT Imaging

doi: 10.3389/fvets.2019.00124

Figure Lengend Snippet: Subocclusal, transverse microCT image of a tooth affected with Grade 2 infundibular caries and also with a small area of possible grade 3 caries (adjacent dentine also affected) (red arrow) of caudal infundibulum (on left). More extensive Grade 3 caries is present in the rostral infundibulum (on right) (yellow arrows). x 2.

Article Snippet: Micro computed tomographic (MicroCT) scanning (XTreme CT, Scanco Medical AG, Bruttisellen, Switzerland) at an isotropic spatial resolution of 82 micrometers was additionally performed on 8 of the above cheek teeth, including Triadan 08 ( n = 1), 09 ( n = 6), 10 ( n = 1) from age groups <5 y.o. ( n = 2); 5–10 y.o. ( n = 2); 10–15 y.o. ( n = 1); 15–20 y.o. ( n = 2); >20 y.o. ( n = 1).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: A Computed Tomographic (CT) and Pathological Study of Equine Cheek Teeth Infundibulae Extracted From Asymptomatic Horses. Part 1: Prevalence, Type and Location of Infundibular Lesions on CT Imaging

doi: 10.3389/fvets.2019.00124

Figure Lengend Snippet: Transverse (x2 magnification, on left) and longitudinal (no magnification, on right) microCT images of the same cheek tooth with small (circa 1 mm wide in the palato-buccal plane) occlusal central linear cemental defects of both infundibulae on the transverse image, considered as innocuous “central vascular channels.” However, at other subocclusal sites on the longitudinal image, one of these central linear defects is up to 3 mm wide (X2 magnification for transverse image when width in bucco-palatal plane = 4.4 cm; X1 magnification for longitudinal image).

Article Snippet: Micro computed tomographic (MicroCT) scanning (XTreme CT, Scanco Medical AG, Bruttisellen, Switzerland) at an isotropic spatial resolution of 82 micrometers was additionally performed on 8 of the above cheek teeth, including Triadan 08 ( n = 1), 09 ( n = 6), 10 ( n = 1) from age groups <5 y.o. ( n = 2); 5–10 y.o. ( n = 2); 10–15 y.o. ( n = 1); 15–20 y.o. ( n = 2); >20 y.o. ( n = 1).

Techniques:

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming

doi: 10.1016/j.bioactmat.2025.11.039

Figure Lengend Snippet: Continuous intraosseous administration of SCS prevents glucocorticoid-induced bone degeneration. ( A ) Schematic illustration of the glucocorticoid (GC; MPS)-induced bone deterioration and intraosseous SCS treatment. ( B-D ) Representative H&E staining images of the femur at 6 weeks (B). Magnified views of the cortical bone and trabecular bone in the marrow cavity are shown on the right. Solid arrows indicate normal osteocytes, while hollow arrows indicate empty osteocyte lacunae. Quantification of empty lacunae ratios in cortical bone (C) and trabecular bone (D). n = 6 biological replicates. (Scale bars, 500 μm and 25 μm) ( E-H ) Representative immunofluorescence staining of OPN + mature osteoblasts, osteolectin + osteoprogenitors, and VE-cadherin + endothelial cells (ECs) in femur at 6 weeks (E), and corresponding quantifications (F–H). n = 6 biological replicates. (Scale bars, 100 μm and 20 μm) ( I and J ) Representative flow cytometry plots of capillary subtypes in the femur (I), with quantification of CD45 − Ter119 − CD31 hi Emcn hi ECs (J). n = 6 biological replicates. ( K and L ) Flow cytometry plots showing Sca-1 hi CD31 hi arteriolar ECs (K), and corresponding quantification (L). n = 6 biological replicates. ( M and N ) Representative micro-CT 3D images of the femur (M). Quantitative analysis of percent bone volume (BV/TV) (N). n = 6 biological replicates. (Scale bars, 1.5 mm, 600 μm and 545 μm) ( O and P ) ELISA analysis of VEGF (O) and PDGF-BB (P) levels in bone marrow supernatant and peripheral serum from PBS- and SCS-treated groups at week 6. n = 6 biological replicates. ( Q ) ELISA quantification of the osteogenic factor osteocalcin in peripheral serum at week 6. n = 6 biological replicates. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns, not significant. Statistical significance was determined using one-way ANOVA with Tukey's post hoc test ( C, D, F, G, H, J, L, N, O, P and Q ).

Article Snippet: High-resolution micro-computed tomography (Micro-CT) scanning was performed using the Skyscan 1272 system (Skyscan).

Techniques: Staining, Immunofluorescence, Flow Cytometry, Micro-CT, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming

doi: 10.1016/j.bioactmat.2025.11.039

Figure Lengend Snippet: SCS targets downstream senescent lineage commitment of bone marrow MSCs to mitigate GC-induced bone deterioration. ( A ) Schematic diagram illustrating the experimental design: CD45 − Ter119 − CD31 − LepR + MSCs isolated from mice co-treated with SCS and MPS for 7 days were subjected to in vitro lineage-competitive differentiation, followed by DEX-induced senescence in lineage-mixed cells. These cells were then adoptively transplanted into healthy bone marrow cavity to assess bone deterioration development. ( B ) Representative H&E-stained images of the femur 12 weeks after adoptive transfer. PBS-DEX group: LepR + MSCs from PBS and MPS co-treated mice subjected to in vitro lineage differentiation and DEX-induced senescence, followed by transplantation. SCS-DEX group: LepR + MSCs from SCS and MPS co-treated mice processed similarly. PBS group: solvent control without cell transplantation. Solid arrows indicate intact osteocytes; hollow arrows indicate empty lacunae. (Scale bars, 250 μm and 25 μm) ( C – E ) Quantitative analysis of marrow hypertrophic adipocyte diameter (C), proportion of empty osteocyte lacunae in trabecular bone (D), and adipocyte number (E) in the metaphysis 12 weeks post-transplantation. n = 19 biological replicates (C), n = 6 biological replicates (D), n = 8 biological replicates (E). ( F ) Quantification of empty lacunae in epiphysis at 12 weeks post-transplantation. n = 6 biological replicates. ( G – I ) Representative flow cytometry plots of capillary ECs subtypes in the femur at 12 weeks (G), with quantification of CD45 − Ter119 − CD31 hi Emcn hi ECs (H) and CD45 − Ter119 − CD31 lo Emcn lo ECs (I). n = 6 biological replicates. ( J and K ) Representative flow cytometry plots (J) and corresponding quantification (K) of CD45 − Ter119 − Sca-1 hi CD31 hi arteriolar ECs in the femur at 12 weeks post-transplantation. n = 6 biological replicates. ( L ) Representative micro-CT images of the femur at 12 weeks post-transplantation across different treatment groups. (Scale bars, 1.5 mm and 500 μm) ( M – P ) Quantitative analysis of bone parameters in the metaphysis: bone mineral density (BMD) (M), percent bone volume (BV/TV) (N), trabecular separation (Tb.Sp) (O), and trabecular number (Tb.N) (P). n = 6 biological replicates. ( Q ) Serum ELISA analysis of the osteogenic marker osteocalcin at 12 weeks post-transplantation. n = 6 biological replicates. ( R and S ) ELISA analysis of PDGF-BB (R) and VEGF (S) in both bone marrow supernatant and peripheral serum at 12 weeks post-transplantation. n = 6 biological replicates. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns, not significant. Statistical significance was determined using one-way ANOVA with Tukey's post hoc test ( C, D, E, F, H, I, K, M, N, O, P, Q, R and S ).

Article Snippet: High-resolution micro-computed tomography (Micro-CT) scanning was performed using the Skyscan 1272 system (Skyscan).

Techniques: Isolation, In Vitro, Staining, Adoptive Transfer Assay, Transplantation Assay, Solvent, Control, Flow Cytometry, Micro-CT, Enzyme-linked Immunosorbent Assay, Marker