mhc i Search Results


anti mhc i monoclonal antibody  (Kingfisher Biotech)
91
Kingfisher Biotech anti mhc i monoclonal antibody
Anti Mhc I Monoclonal Antibody, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/Anti-bovine+MHC+I+monoclonal+antibody/pmc05309937-389-19-10
Average 91 stars, based on 1 article reviews
anti mhc i monoclonal antibody - by Bioz Stars, 2026-09
91/100 stars
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mhc i  (Boster Bio)
90
Boster Bio mhc i
Mhc I, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/Anti-HLA-Aw32+%26+HLA-A25+(MHC+I)+Monoclonal+Antibody/pmc07285657-443-21-24
Average 90 stars, based on 1 article reviews
mhc i - by Bioz Stars, 2026-09
90/100 stars
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cef mhc-i control peptide pool  (AnaSpec)
90
AnaSpec cef mhc-i control peptide pool
Cef Mhc I Control Peptide Pool, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/cef+mhc+i+control+peptide+pool/pmc08402406-73-42-44
Average 90 stars, based on 1 article reviews
cef mhc-i control peptide pool - by Bioz Stars, 2026-09
90/100 stars
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mouse mhc-i knockdown adenovirus  (Shanghai GenePharma)
90
Shanghai GenePharma mouse mhc-i knockdown adenovirus
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Mouse Mhc I Knockdown Adenovirus, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/mouse+mhc+i+knockdown+adenovirus/pmc08374590-75-13-19
Average 90 stars, based on 1 article reviews
mouse mhc-i knockdown adenovirus - by Bioz Stars, 2026-09
90/100 stars
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dna-binding protein (dbp 419–427 : falsnaedl)  (GenScript corporation)
90
GenScript corporation dna-binding protein (dbp 419–427 : falsnaedl)
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Dna Binding Protein (Dbp 419–427 : Falsnaedl), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/mhc+i+restricted+peptide+of+adenovirus+dna+binding+protein++dbp+419+427+++falsnaedl+/pmc02953831-207-11-21
Average 90 stars, based on 1 article reviews
dna-binding protein (dbp 419–427 : falsnaedl) - by Bioz Stars, 2026-09
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fluorescein isothiocyanate (fitc)-conjugated anti-human major histocompatibility complex class i (mhc-i) antibody  (Becton Dickinson)
90
Becton Dickinson fluorescein isothiocyanate (fitc)-conjugated anti-human major histocompatibility complex class i (mhc-i) antibody
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Fluorescein Isothiocyanate (Fitc) Conjugated Anti Human Major Histocompatibility Complex Class I (Mhc I) Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/fluorescein+isothiocyanate++fitc++conjugated+anti+human+major+histocompatibility+complex+class+i++mhc+i++antibody/pm17615261-68-18-29
Average 90 stars, based on 1 article reviews
fluorescein isothiocyanate (fitc)-conjugated anti-human major histocompatibility complex class i (mhc-i) antibody - by Bioz Stars, 2026-09
90/100 stars
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antibodies against mhc-i apc mouse anti-human hla-abc clone g46-2.6  (Pharmagen gmbh)
90
Pharmagen gmbh antibodies against mhc-i apc mouse anti-human hla-abc clone g46-2.6
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Antibodies Against Mhc I Apc Mouse Anti Human Hla Abc Clone G46 2.6, supplied by Pharmagen gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/antibodies+against+mhc+i+apc+mouse+anti+human+hla+abc+clone+g462+6/pmc12123720-46-14-31
Average 90 stars, based on 1 article reviews
antibodies against mhc-i apc mouse anti-human hla-abc clone g46-2.6 - by Bioz Stars, 2026-09
90/100 stars
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rat anti-mhc-i  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH rat anti-mhc-i
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Rat Anti Mhc I, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/rat+anti+mhc+i/pmc10616105-330-162-168
Average 90 stars, based on 1 article reviews
rat anti-mhc-i - by Bioz Stars, 2026-09
90/100 stars
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mhc-i  (Becton Dickinson)
90
Becton Dickinson mhc-i
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Mhc I, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/mhc+i+antibody/pmc05573767__mmc1-78-12-13
Average 90 stars, based on 1 article reviews
mhc-i - by Bioz Stars, 2026-09
90/100 stars
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anti-hla-a,b,c antibody g46_2.6  (Becton Dickinson)
90
Becton Dickinson anti-hla-a,b,c antibody g46_2.6
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Anti Hla A,B,C Antibody G46 2.6, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/hla+a+b+c+++mhc+i++555553+/pm37156915-390-36-40
Average 90 stars, based on 1 article reviews
anti-hla-a,b,c antibody g46_2.6 - by Bioz Stars, 2026-09
90/100 stars
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mhc-i (h-2k[d])-fitc  (Becton Dickinson)
90
Becton Dickinson mhc-i (h-2k[d])-fitc
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Mhc I (H 2k[D]) Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/mhc+i++h+2k+d+++fitc+antibody/us09745552-666-22-30
Average 90 stars, based on 1 article reviews
mhc-i (h-2k[d])-fitc - by Bioz Stars, 2026-09
90/100 stars
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mhc-i-tetramers, consisting of tetramerized and pe-labelled hla-a*02:01 monomers carrying the nlvpmvatv peptide cmvpp65 495 − 503  (immunAware ApS)
90
immunAware ApS mhc-i-tetramers, consisting of tetramerized and pe-labelled hla-a*02:01 monomers carrying the nlvpmvatv peptide cmvpp65 495 − 503
Expression <t>of</t> <t>MHC-I</t> in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.
Mhc I Tetramers, Consisting Of Tetramerized And Pe Labelled Hla A*02:01 Monomers Carrying The Nlvpmvatv Peptide Cmvpp65 495 − 503, supplied by immunAware ApS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mhc+i/mhc+i+tetramers++consisting+of+tetramerized+and+pe+labelled+hla+a+02+01+monomers+carrying+the+nlvpmvatv+peptide+cmvpp65+495+++503/pmc12224750-49-8-25
Average 90 stars, based on 1 article reviews
mhc-i-tetramers, consisting of tetramerized and pe-labelled hla-a*02:01 monomers carrying the nlvpmvatv peptide cmvpp65 495 − 503 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Expression of MHC-I in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: Expression of MHC-I in dopaminergic neurons is increased in Parkinson's disease models. (A) MHC-I expression in SH-SY5Y cells after being incubated with MPP + (0.1 mmol/mL) and INF-γ (100 ng/mL) for 24 hours. (B) MHC-I expression in SH-SY5Y cells after different concentrations of MPP + treatment for 24 hours. (C) MHC-I expression in SH-SY5Y cells after different incubation times with MPP + . (D) Fluorescent images showing MHC-I (green) expression in SH-SY5Y cells. Scale bar: 20 μm. (E) Confocal immunofluorescent labeling of TH (green) and MHC-I (red) in the substantia nigra of mice in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (F, G) Numbers of TH + neurons in the substantia nigra of mice and the proportion of TH + neurons with MHC-I expression. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001. INF-γ: Interferon-γ; MHC-I: major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Incubation, Labeling, Injection

Expression of MHC-I causes immune attacks in the substantia nigra of Parkinson's disease model mice. (A) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the substantia nigra of the mouse midbrain in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (B) Numbers of CD8 + T cells per mm 2 . (C) Representative immunofluorescence double staining for the nucleus (DAPI, blue) and TH (red) in the substantia nigra region. Scale bar: 2 mm. (D) Numbers of TH + neurons in the substantia nigra of mice. (E) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the MPTP + Ad-MHC-1-shRNA group. The red arrow indicates the side that was injected with Ad-MHC-1-shRNA, while the yellow arrow indicates the side that was injected with Ad-NC. Scale bar: 50 μm. (F, G) Numbers of CD8 + T cells and MHC-1-positive neurons per mm 2 . * P < 0.05, ** P < 0.01, *** P < 0.001. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; MHC-I: major histocompatibility complex class I; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; NC: negative control; TH: tyrosine hydroxylase.

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: Expression of MHC-I causes immune attacks in the substantia nigra of Parkinson's disease model mice. (A) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the substantia nigra of the mouse midbrain in the control group, 1-day-after-injection group, and 7-days-after-injection group. Scale bar: 50 μm. (B) Numbers of CD8 + T cells per mm 2 . (C) Representative immunofluorescence double staining for the nucleus (DAPI, blue) and TH (red) in the substantia nigra region. Scale bar: 2 mm. (D) Numbers of TH + neurons in the substantia nigra of mice. (E) Immunofluorescence triple staining for TH (red), MHC-I (green), and CD8 + T cells (pink) in the MPTP + Ad-MHC-1-shRNA group. The red arrow indicates the side that was injected with Ad-MHC-1-shRNA, while the yellow arrow indicates the side that was injected with Ad-NC. Scale bar: 50 μm. (F, G) Numbers of CD8 + T cells and MHC-1-positive neurons per mm 2 . * P < 0.05, ** P < 0.01, *** P < 0.001. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). DAPI: 4′,6-Diamidino-2-phenylindole; MHC-I: major histocompatibility complex class I; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; NC: negative control; TH: tyrosine hydroxylase.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Staining, Injection, Double Staining, shRNA, Negative Control

PINK1 is related to MHC-I expression in SH-SY5Y cells. (A) Flow cytometric analysis of MHC-I expression in neuron membranes. (B) Quantification of the flow cytometric analysis results. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05. (C) Western blot assay of PINK1 expression in SH-SY5Y cells that had been transfected with control or PINK1 siRNA. (D) Fluorescence images showing the expression of MHC-1 (green) in SH-SY5Y cells. Scale bar: 5 μm. The arrow indicates MHC-I protein presenting on the cell surface. MHC-I: Major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; PINK1: PTEN-induced kinase 1.

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: PINK1 is related to MHC-I expression in SH-SY5Y cells. (A) Flow cytometric analysis of MHC-I expression in neuron membranes. (B) Quantification of the flow cytometric analysis results. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05. (C) Western blot assay of PINK1 expression in SH-SY5Y cells that had been transfected with control or PINK1 siRNA. (D) Fluorescence images showing the expression of MHC-1 (green) in SH-SY5Y cells. Scale bar: 5 μm. The arrow indicates MHC-I protein presenting on the cell surface. MHC-I: Major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; PINK1: PTEN-induced kinase 1.

Article Snippet: To induce the MHC-I knockdown model (Ye et al., 2018), we used a mouse MHC-I knockdown adenovirus designed by GenePharma Co. (Shanghai, China).

Techniques: Expressing, Western Blot, Transfection, Fluorescence