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Image Search Results
Journal: Cancer Medicine
Article Title: DEPDC1 as a crucial factor in the progression of human osteosarcoma
doi: 10.1002/cam4.5340
Figure Lengend Snippet: DEPDC1 highly expressed in GEO database bioinformatics analysis, and clinical osteosarcoma specimens and osteosarcoma cell lines. (A) Heat map across the human osteosarcoma among GEO database. (B) Bar chart showing expression levels of DEPDC1 . (C) Representative immunohistochemical (IHC) staining (scale bar 100 μm) against DEPDC1 protein on osteosarcoma patient tumor biopsy sections (left panel) versus adjacent normal tissues (right panel), with quantification bar chart at the bottom. (D) The plot of real‐time PCR analysis of DEPDC1 expression on osteosarcoma cells (HOS, MG‐63, and Saos‐2) and normal osteoblast controls (hFOB1.19). (E) Representative western blot images of cell lysates with antibodies against DEPDC1 on the top row, with the lower panel indicating the normalized quantification (*** p < 0.01, **** p < 0.001).
Article Snippet: Human osteosarcoma cell lines HOS(#20210713–17),
Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Western Blot
Journal: Cancer Medicine
Article Title: DEPDC1 as a crucial factor in the progression of human osteosarcoma
doi: 10.1002/cam4.5340
Figure Lengend Snippet: Downregulation of DEPDC1 inhibiting osteosarcoma cell proliferation and migration, and promoting apoptosis. (A) Western blotting and (B) real‐time PCR showing down‐regulation of DEPDC1 in sh DEPDC1 groups. (C) MTT assay showing diminishment of cell proliferations after knockdown of DEPDC1 expression in HOS and MG‐63 cells. (D) EdU assay showing knockdown of DEPDC1 expression could lead to cell cycle arrest in osteosarcoma cell lines. (E) Flow cytometry quantification of apoptotic cell percentages among groups. (F) Cell colony visualization and quantification of HOS and MG‐63 cells with control or sh DEPDC1 interventions. Cell scratch assay ( G) and Transwell assay (H) to evaluate cell migration and cell invasion capacities of HOS and MG‐63 osteosarcoma cells after silencing of DEPDC1 expression. (*** p < 0.01, **** p < 0.001).
Article Snippet: Human osteosarcoma cell lines HOS(#20210713–17),
Techniques: Migration, Western Blot, Real-time Polymerase Chain Reaction, MTT Assay, Expressing, EdU Assay, Flow Cytometry, Wound Healing Assay, Transwell Assay