Journal: Cell metabolism

Article Title: Epigenetic Control of Mitochondrial Fission Enables Self-Renewal of Stem-like Tumor Cells in Human Prostate Cancer.

doi: 10.1016/j.cmet.2019.05.004

Figure Lengend Snippet: Figure 6. BRD4 Promotes MFF Transcription in Prostate Cancer (A and B) MFF and BRD4 protein expression by immunohistochemistry in hormone-naive primary and hormone-refractory metastatic human prostate tumors. Representative images (A) and quantitative scores (B) for MFF and BRD4. (C) MFF expression and localization in DU145 cells determined by immunofluorescence microscopy and co-staining with MitoTracker Orange. Nuclei were stained with DAPI. (D) MFF expression by RT-qPCR in adherent (Adh) and tumor-sphere (TS) cells. (E) MFF promoter occupancy by BRD4 determined by chromatin immunoprecipitation and qPCR in adherent and tumor-sphere DU145 cells untreated or treated with OTX015 (100 and 1000 nM) for 72 h. (F) MFF mRNA in adherent and tumor-sphere cells at 72 h after OTX015 treatment. (G and H) MFF mRNA (G) and protein (H) level in control and OTX015-treated tumor xenografts. * p < 0.01 by t test. See also Figure S6.

Article Snippet: Vectors for expression of MFF (GFP-MFF, #49153; Flag-MFF, #74379), dominant negative DRP1K38A mutant (#26049) and BRD4 (p6349 MSCV-CMV-Flag-HA-Brd4 1224- 1362, #31355) were obtained from Addgene (www.addgene.org).

Techniques: Expressing, Immunohistochemistry, Microscopy, Staining, Quantitative RT-PCR, Chromatin Immunoprecipitation, Control