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Image Search Results
Journal: Scientific Reports
Article Title: DNA methylation markers panel can improve prediction of response to neoadjuvant chemotherapy in luminal B breast cancer
doi: 10.1038/s41598-020-66197-1
Figure Lengend Snippet: A representative example of multi-locus MSRE-PCR with primers pool P3 for the assessment of DNA methylation at the CpG islands of the genes discriminating good and poor response of tumors to neoadjuvant chemotherapy. Even lanes contain PCR products from tumor DNA samples hydrolyzed with the BstHHI methylation sensitive restriction endonuclease, and odd ones contain PCR products of intact tumor DNA samples (mock digestion without BstHHI). M, DNA molecular weight marker pUC19/MspI.
Article Snippet: To analyze DNA methylation at the CpG islands of individual genes, we treated genomic DNA from tissue samples with
Techniques: DNA Methylation Assay, Methylation, Molecular Weight, Marker