Journal: Virchows Archiv

Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma

doi: 10.1007/s00428-025-04132-3

Figure Lengend Snippet: Representative images of an EC with MLH1 deficiency, absence of MLH1 promoter methylation, and positive EPM2 AIP1 immunostaining. The tumor exhibited biallelic somatic alteration of MLH1 ; one pathogenic variant in MLH1 ( NM_000249.4 ): p.Glu633 Ter (c.1897G > T), classified as a stop-gained mutation, truncating the MLH1 protein, as well as loss of heterozygosity (LOH) detected in chromosomal region 3p22.2-q27.1

Article Snippet: In summary, results show concordance between MLH1 promoter methylation analysis by pyrosequencing and EPM2 AIP1 IHC, with 100% EPM2 AIP1 negativity in the 70 cases that exhibited MLH1 promoter methylation by pyrosequencing.

Techniques: Methylation, Immunostaining, Variant Assay, Mutagenesis

Journal: Virchows Archiv

Article Title: EPM2 AIP1 immunohistochemistry as a surrogate of promoter methylation analysis in endometrial carcinoma

doi: 10.1007/s00428-025-04132-3

Figure Lengend Snippet: Representative images of an EC with MLH1 with a methylation score close to the cut-off. The initial biopsy A showed negative EPM2 AIP1 immunostaining and a methylation score of 9.8. In the surgical specimen B , EPM2 AIP1 immunostaining was negative, but the methylation score increased to 60% after tumor microdissection

Article Snippet: In summary, results show concordance between MLH1 promoter methylation analysis by pyrosequencing and EPM2 AIP1 IHC, with 100% EPM2 AIP1 negativity in the 70 cases that exhibited MLH1 promoter methylation by pyrosequencing.

Techniques: Methylation, Immunostaining, Laser Capture Microdissection

Journal: Gastroenterology and Hepatology From Bed to Bench

Article Title: The epigenetic influence of diet-induced gut microbiome changes in precision nutrition – a systematic review

doi: 10.22037/ghfbb.v18i3.3136

Figure Lengend Snippet: Epigenetic Changes in Gene Expression — This figure illustrates two key epigenetic mechanisms that regulate gene expression: DNA methylation and histone modifications. DNA methylation involves the addition of methyl groups to the promoter region of a gene, inhibiting transcription and leading to gene silencing. Histone modifications, including acetylation and methylation, alter chromatin structure and gene accessibility; acetylation relaxes chromatin, promoting gene activation, while methylation can either enhance or suppress transcription depending on its context. These epigenetic modifications are dynamic and influenced by environmental factors, such as diet, with implications for metabolism, inflammation, and disease susceptibility.

Article Snippet: Sun, et al. (21) , DNA methylation analysis (DREAM sequencing, bisulfite pyrosequencing, qRT-PCR) in a microbiota-inflammation model. , Difficulty separating inflammation’s role in epigenetic changes. , Microbiota and inflammation influenced DNA methylation at CpG sites. , DNA methylation at CpG sites. , Not assessed. , Multi-model approach, deep-sequencing of methylation changes. , Moderate , 2023.

Techniques: Gene Expression, DNA Methylation Assay, Methylation, Activation Assay

Journal: Gastroenterology and Hepatology From Bed to Bench

Article Title: The epigenetic influence of diet-induced gut microbiome changes in precision nutrition – a systematic review

doi: 10.22037/ghfbb.v18i3.3136

Figure Lengend Snippet: Epigenetic Modifications in Metabolic Health and Disease Prevention – This figure illustrates the impact of diet-induced changes in gut microbiome composition on epigenetic modifications and their subsequent effects on metabolic health. The dietary components, such as fiber-rich, polyphenol-rich, and high-fat diets, are shown to influence microbiome diversity and promote the production of metabolites like short-chain fatty acids (SCFAs), which modulate epigenetic markers (e.g., DNA methylation, histone modifications) and gene expression related to inflammation, metabolism, and disease susceptibility. Diets rich in fiber and polyphenols are associated with beneficial microbiome shifts and favorable epigenetic modifications that support metabolic health and reduce the risk of metabolic diseases like obesity and insulin resistance. In contrast, Western-style diets high in fat and processed foods contribute to dysbiosis, inflammation, and adverse epigenetic changes, which may increase the risk of developing chronic metabolic disorders, including obesity and type 2 diabetes.

Article Snippet: Sun, et al. (21) , DNA methylation analysis (DREAM sequencing, bisulfite pyrosequencing, qRT-PCR) in a microbiota-inflammation model. , Difficulty separating inflammation’s role in epigenetic changes. , Microbiota and inflammation influenced DNA methylation at CpG sites. , DNA methylation at CpG sites. , Not assessed. , Multi-model approach, deep-sequencing of methylation changes. , Moderate , 2023.

Techniques: DNA Methylation Assay, Gene Expression, Western Blot