methylated dna Search Results


95
Zymo Research human methylated dna
Human Methylated Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs epimark methylated dna enrichment kit
Epimark Methylated Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research ez dna methylation lightning kit
Ez Dna Methylation Lightning Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research ez dna methylation gold kit zymo research
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Zymo Research ez 96 dna methylation kit
Ez 96 Dna Methylation Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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94
EpigenDx human genomic dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Human Genomic Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylated+dna/Human+High+Methylated+DNA/bio_rxiv__64898__2026__01__21__700899-78-4-11
Average 94 stars, based on 1 article reviews
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94
EpigenDx low methylated dna
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Low Methylated Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Active Motif global dna methylation line 1 kit
Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and <t>DNA</t> were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly <t>increased</t> <t>methylated</t> IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).
Global Dna Methylation Line 1 Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Zymo Research dna zymo methylation kit
Summary of the comparison of characteristics and performance of <t> DNA </t> <t> methylation </t> detection methods based on literature search and obtained benchmarking results.
Dna Zymo Methylation Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylated+dna/EZ+DNA+Methylation+Kit/pmc13639291-57-6-7
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94
Vazyme Biotech Co bisulfite conversion
Summary of the comparison of characteristics and performance of <t> DNA </t> <t> methylation </t> detection methods based on literature search and obtained benchmarking results.
Bisulfite Conversion, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylated+dna/EpiArt+DNA+Methylation+Library+Kit+for+Illumina+V3/pm41786706-396-44-49
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94
EpigenDx high methylated genomic dna
Summary of the comparison of characteristics and performance of <t> DNA </t> <t> methylation </t> detection methods based on literature search and obtained benchmarking results.
High Methylated Genomic Dna, supplied by EpigenDx, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methylated+dna/Monkey+High+Methylated+DNA/pmc07725942-254-26-30
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Zymo Research ez 96 dna methylation gold kit
Summary of the comparison of characteristics and performance of <t> DNA </t> <t> methylation </t> detection methods based on literature search and obtained benchmarking results.
Ez 96 Dna Methylation Gold Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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Image Search Results


Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and DNA were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly increased methylated IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).

Journal: bioRxiv

Article Title: Epigenetic Regulation of Inflammation by Dopamine in Primary Human Macrophages

doi: 10.64898/2026.01.21.700899

Figure Lengend Snippet: Primary human monocyte-derived macrophages (hMDM) from sixteen donors were treated with dopamine (10 -6 M) for 3 hours with or without pre-treatment using hypomethylating agents 5-aza-2’-deoxycytidine (dAZA; 10 -6 M), 5-azacytidine (AZA; 10 -6 M), or vehicle control. RNA and DNA were isolated for analysis of IL-1β gene expression by qPCR and IL-1β DNA methylation, respectively. (A) Dopamine increased IL-1β mRNA expression, an effect inhibited by dAZA (n= 7-8 donors, *p<0.05). (B) AZA pretreatment produced a similar inhibitory effect on dopamine-induced IL-1β expression, trending toward statistical significance (n=5-6, p=0.0513). (C) Dopamine treatment significantly increased methylated IL-1β levels compared to vehicle control (n=16, p<0.05). (D) Lipopolysaccharide (LPS) increased IL-1β DNA methylation; however, this effect did not reach statistical significance in this donor cohort (n=16). (E) LPS-induced changes in IL-1β DNA methylation were significantly correlated with dopamine-induced changes within the same donors, indicating consistent donor-specific responsiveness across stimuli (n=16, **p<0.01).

Article Snippet: Both high-methylated and low-methylated human genomic DNA (80-8061-HGHM5 and 80-8062-HGUM5 from EpigenDx) were bisulfite treated and used as controls for the primer sets.

Techniques: Derivative Assay, Control, Isolation, Gene Expression, DNA Methylation Assay, Expressing, Produced, Methylation

Summary of the comparison of characteristics and performance of  DNA   methylation  detection methods based on literature search and obtained benchmarking results.

Journal: Frontiers in Epigenetics and Epigenomics

Article Title: Benchmark of the Oxford Nanopore, EM-seq, and HumanMethylationEPIC BeadChip for the detection of the 5mC sites in cancer and normal samples

doi: 10.3389/freae.2024.1362926

Figure Lengend Snippet: Summary of the comparison of characteristics and performance of DNA methylation detection methods based on literature search and obtained benchmarking results.

Article Snippet: Bisulfite conversion was performed using the DNA Zymo Methylation Kit (Zymo Research, United States) for 1,000 ng of genomic DNA.

Techniques: Comparison, DNA Methylation Assay, Dispersion