Journal: Poultry science

Article Title: Immune synergistic mechanism of recombinant plasmid adjuvant containing chicken IL-4 and IL-2 fusion genes on chicken coccidia live vaccine.

doi: 10.1016/j.psj.2023.103204

Figure Lengend Snippet: Figure 5. B-lymphocyte activation and proliferation level. (A) Activated B lymphocytes in cecal tonsils, spleen, thymus, and bursa of Fabricius were detected by MGP staining under fluorescence microscopy on 28th-day postinoculation. (B) Levels of positive B cells were detected in different organs on 7th-, 14th-, and 28th-day postinoculation, respectively.

Article Snippet: The activated B lymphocytes were stained by the methyl green pyronine (MGP) staining solution kit (Beyotime, Shanghai, China).

Techniques: Activation Assay, Staining, Microscopy

Journal: Bioconjugate chemistry

Article Title: A Trimethoprim Conjugate of Thiomaltose Has Enhanced Antibacterial Efficacy In Vivo

doi: 10.1021/acs.bioconjchem.8b00177

Figure Lengend Snippet: (A) Thiomaltose trimethoprim (TM-TMP): A new trimethoprim prodrug with enhanced water solubility that targets bacteria. (B) Proposed cleavage of TM-TMP in the presence of exogenous thiols.

Article Snippet: Control groups included mice untreated with any drug, and mice treated with trimethoprim (Chem-Impex International Inc. Wood Dale, IL), while the experimental group was treated with thiomaltose-trimethoprim (TM-TMP).

Techniques: Solubility, Bacteria

Journal: Bioconjugate chemistry

Article Title: A Trimethoprim Conjugate of Thiomaltose Has Enhanced Antibacterial Efficacy In Vivo

doi: 10.1021/acs.bioconjchem.8b00177

Figure Lengend Snippet: Synthesis of thiomaltose-trimethoprim (TM-TMP). (a) DCM, TEA, rt, 55%. (b) DCC, DMAP, TEA, DMF, rt, 29%. (c) CuI, DIPEA, DMSO, rt, 65%.

Article Snippet: Control groups included mice untreated with any drug, and mice treated with trimethoprim (Chem-Impex International Inc. Wood Dale, IL), while the experimental group was treated with thiomaltose-trimethoprim (TM-TMP).

Techniques: