Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: All-trans retinoic acid and COX-2 cross-talk to regulate BMP9-induced osteogenic differentiation via Wnt/β-catenin in mesenchymal stem cells.

doi: 10.1016/j.biopha.2019.109279

Figure Lengend Snippet: Fig. 2. Effects of ATRA and/or COX-2 inhibitor on osteogenic markers induced by BMP9 in C3H10T1/2 cells. (A) ALP staining showed the effect of NS398 and/or ATRA on ALP activity induced by BMP9 in C3H10T1/2 cells (NS398: COX-2 specific inhibitor). (B) Quantification of ALP staining showed the effect of NS398 and/or ATRA on ALP activity induced by BMP9 in C3H10T1/2 cells (“**” p < 0.01 vs control; “#” p < 0.05 vs group treated with BMP9 only). (C) Western blot assay showed the effect of NS398 and/or ATRA on Runx2 induced by BMP9 in C3H10T1/2 cells. (D) Quantification of Western blot assay showed the effect of NS398 and/or ATRA on Runx2 induced by BMP9 in C3H10T1/2 cells (“*” p < 0.05, “**” p < 0.01 vs control; “#” p < 0.05, “##” p < 0.01 vs group treated with BMP9 only). (E) Western blot assay showed the effect of NS398 and/or ATRA on OPN induced by BMP9 in C3H10T1/2 cells. (F) Quantification of Western blot assay showed the effect of NS398 and/or ATRA on OPN induced by BMP9 in C3H10T1/2 cells (“*” p < 0.05, “**” p < 0.01 vs control; “#” p < 0.05, “##” p < 0.01 vs group treated with BMP9 only). (G) Alizarin Red S staining showed the effect of NS398 and/or ATRA on mineralization induced by BMP9 in C3H10T1/2 cells. (H) Quantification of Alizarin Red S staining showed the effect of NS398 and/or ATRA on mineralization induced by BMP9 in C3H10T1/2 cells (“**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9 only).

Article Snippet: COX-2 specific inhibitor NS398 (Cat# T3214) was ordered from Targetmol (Shanghai, China), and RARa inhibitor AGN196996 (Cat# HY-16682) was bought from MCE (Shanghai, China).

Techniques: Staining, Activity Assay, Control, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: All-trans retinoic acid and COX-2 cross-talk to regulate BMP9-induced osteogenic differentiation via Wnt/β-catenin in mesenchymal stem cells.

doi: 10.1016/j.biopha.2019.109279

Figure Lengend Snippet: Fig. 3. Effects of COX-2 specific inibitor and/or BMP9 on Wnt/β-catenin in C3H10T1/2 cells. (A) Western blot assay showed the effect of BMP9 and/or NS398 on the protein level of β-catenin in C3H10T1/2 cells (NS398: COX-2 specific inhibitor). (B) Quantification of Western blot assay showed the effect of BMP9 and/or NS398 on the protein level of β-catenin in C3H10T1/2 cells (“**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (C) Western blot assay showed the effect of BMP9 and/or NS398 on protein level of β-catenin in C3H10T1/2 cells’ cytoplasm. (D) Quantification of Western blot assay showed the effect of BMP9 and/or NS398 on protein level of β-catenin in C3H10T1/2 cells’ nucleus (“*” p < 0.05 vs control; “**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (E) Western blot assay showed the effect of BMP9 and/or NS398 on protein level of β-catenin in C3H10T1/2 cells’ nucleus. (F) Quantification of Western blot assay showed the effect of BMP9 and/or NS398 on protein level of β-catenin in C3H10T1/2 cells’ nucleus (“*” p < 0.05 vs control; “**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (G) Real-time PCR assay showed the effect of BMP9 and/ or NS398 on mRNA level of β-catenin in C3H10T1/2 cells (“**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (H) Real-time PCR assay showed the effect of BMP9 and/or NS398 on mRNA level of c-Myc in C3H10T1/2 cells (“*” p < 0.05 vs control; “**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (I) Real-time PCR assay showed the effect of BMP9 and/or NS398 on mRNA level of Id1 in C3H10T1/2 cells (“**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9).

Article Snippet: COX-2 specific inhibitor NS398 (Cat# T3214) was ordered from Targetmol (Shanghai, China), and RARa inhibitor AGN196996 (Cat# HY-16682) was bought from MCE (Shanghai, China).

Techniques: Western Blot, Control, Real-time Polymerase Chain Reaction

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: All-trans retinoic acid and COX-2 cross-talk to regulate BMP9-induced osteogenic differentiation via Wnt/β-catenin in mesenchymal stem cells.

doi: 10.1016/j.biopha.2019.109279

Figure Lengend Snippet: Fig. 6. Effects of retinoic acid signaling and/or COX-2/CREB on expression of β-catenin induced by BMP9 in C3H10T1/2 cells. (A) Western blot assay showed the effect of BMP9 and/or NS398 on CREB and p-CREB level in C3H10T1/2 cells (NS398: COX-2 specific inhibitor). (B) Quantification of Western blot assay showed the effect of BMP9 and/or NS398 on CREB and p-CREB level in C3H10T1/2 cells (“*” p < 0.05 vs control; “**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (C) Western blot assay showed the effect of BMP9 and/or AGN on CREB and p-CREB level in C3H10T1/2 cells (AGN: AGN196996, RARa specific inhibitor). (D) Quantification of Western blot assay showed the effect of BMP9 and/or AGN on CREB and p-CREB level in C3H10T1/2 cells (“**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (E) Western blot assay showed the effect of BMP9, NS398 and/or ATRA on CREB and p-CREB level in C3H10T1/2 cells. (F) Quantification of Western blot assay showed the effect of BMP9, NS398 and/or ATRA on CREB and p-CREB level in C3H10T1/2 cells (“*” p < 0.05 vs control; “**” p < 0.01 vs control; “##” p < 0.01 vs group treated with BMP9). (G) ChIP assay showed the recruitment of p-CREB or RXRa at the promoter region of β-catenin in C3H10T1/2 cells. (H) IP assay showed the interaction between p-CREB and RXRa in C3H10T1/2 cells.

Article Snippet: COX-2 specific inhibitor NS398 (Cat# T3214) was ordered from Targetmol (Shanghai, China), and RARa inhibitor AGN196996 (Cat# HY-16682) was bought from MCE (Shanghai, China).

Techniques: Expressing, Western Blot, Control

Journal: Regulatory toxicology and pharmacology : RTP

Article Title: Comparison of PAC and MOAH for understanding the carcinogenic and developmental toxicity potential of mineral oils.

doi: 10.1016/j.yrtph.2022.105193

Figure Lengend Snippet: Fig. 6. Toxicity of a mineral oil is directly proportional to its PAC content by DMSO extract, which is inversely proportional to viscosity and MOAH alkylation degree.

Article Snippet: Thus, to make this distinction, the pharmacopeia UV method uses a DMSO extraction step which concentrates the 3–7 ring PAC (EDQM 2019), so that the measurement reflects the toxicologically relevant aromatic fraction and independent of MOAH molecular weight.

Techniques: Viscosity

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Simplified process flow diagram of the reference methane SCP production process, based on .

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Basis of calculation for the energy requirements of methane SCP production.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Energy requirements of methane SCP production per step in kWh over the dry mass of product.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques: Centrifugation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Range of the share of global resources required to fulfill the minimum global human protein requirements, while accounting for 12% food waste.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Expected ramp-up speed of methane SCP production in terms of the global caloric human requirements fulfilled over time. The results shown reflect the use of the budget of similar industries, including regular and fast construction speeds.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Expected ramp-up speed of methane SCP production in terms of the global protein human requirements fulfilled over time, for different values of the protein content of the SCP product. The results shown reflect the use of the budget of similar industries, including regular- and fast-construction speeds.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Breakdown of the contributions to the wholesale production cost incurred per unit of methane SCP produced.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques: Produced

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Retail cost of methane SCP for different cost scenarios in U.S. dollars per kilogram of dry SCP product.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques: