Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the MMP‐2 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. MMP‐2 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the TNF‐α and IL‐6 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. (A) represents subsequent time points for TNF‐α analysis, and (B) represents subsequent time points for IL‐6 analysis. TNF‐α and IL‐6 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IL‐6: interleukin‐6; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the VEGF‐A levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. VEGF‐A levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; P 4 , progesterone; VEGF‐A, vascular endothelial growth factor‐A.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the IGFBP‐1 secretions in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser+ E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser + E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. IGFBP‐1 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IGFBP‐1, insulin‐like growth factor‐binding protein‐1; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification, Binding Assay

Journal: Cell Death & Disease

Article Title: β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1

doi: 10.1038/s41419-026-08457-y

Figure Lengend Snippet: A , B Gene set enrichment analysis (GSEA) of the TCGA HCC cohort identified significant enrichment of EMT and IL-6/JAK/STAT3 signaling pathways in tumors with high OXCT1 expression. C Western blot analyzed protein levels of OXCT1, STAT3, p-STAT3, Snail, Vimentin, MMP2 and E-cadherin in HCCLM3-OXCT1 or CON cells and in ( D ) Huh7-shOXCT1 or shCON cells. E Western blot analyzed protein levels of OXCT1, STAT3, p-STAT3, Snail, Vimentin, MMP2 and E-Cadherin in HCCLM3 cells treated with vehicle (DMSO) or 10 μM Stattic (STAT3 Inhibitor) for 24 h. F Wound healing assay measured the migratory abilities of the HCCLM3 cells, respectively, treated with vehicle (DMSO) or 10 μM Stattic for 24 h. Quantified data are shown on the right. G Transwell migration and invasion assays of the HCCLM3 cells respectively, treated with vehicle (DMSO) or 10 μM Stattic for 24 h. Quantified data are shown on the right. Data are presented as the mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ns, not significant.

Article Snippet: Primary antibodies against the following proteins were used: β-actin (#20536-1-AP, Proteintech, China), OXCT1 (#12175-1-AP, Proteintech, China), β-catenin (#8480, Cell Signaling Technology, USA), PPARα (#66826-1-Ig, Proteintech, China), HMGCS2 (#ab137043, Abcam, USA), BDH1 (#15417-1-AP, Proteintech, China), LEF1 (#14972-1-AP, Proteintech, China), MMP2 (#10373-2-AP, Proteintech, China), Snai1 (13099-1-AP, Proteintech, China), Vimentin(#60330-1-Ig, Proteintech, China), E-cadherin (HY- P81271 , MCE, China), STAT3 (#9139, Cell Signaling Technology, USA), p-STAT3 (#9145, Cell Signaling Technology, USA).

Techniques: Protein-Protein interactions, Expressing, Western Blot, Wound Healing Assay, Migration

Journal: Cell Death & Disease

Article Title: β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1

doi: 10.1038/s41419-026-08457-y

Figure Lengend Snippet: HCCLM3 cells stably overexpressing β-cateninS33Y (β-catenin) were further infected with shOXCT1 lentivirus to generate cells with concurrent OXCT1 knockdown (β-catenin+shOXCT1). Control (CON), β-catenin, and β-catenin+shOXCT1 cells were used. A Western blot analyzed protein levels of OXCT1 and β-catenin in the CON, β-catenin and β-catenin+shoxct1 groups. B Wound healing assay was performed to measure the migratory abilities of the indicated cell groups. C Transwell migration and invasion assays measured the migratory and invasive abilities of the indicated cell groups. D Western blot analyzed protein levels of STAT3, p-STAT3, MMP2, Snail, Vimentin and E-cadherin in the indicated cell groups. E In tail vein metastasis models, mice ( n = 4 per group) were injected with 2 × 10⁶ cells from each group. Representative livers show metastatic burden (left panel: overview; right panel: magnified views of metastatic lesions). F Western blot analyzed protein levels of STAT3, p-STAT3, MMP2, Vimentin, Snail, E-cadherin, OXCT1, and β-catenin in liver metastatic tissues from each group. G Representative images of liver metastasis with HE staining of the CON, β-catenin, and β-catenin+shoxct1 groups. H Representative images of liver metastasis with Ki67 staining of the CON, β-catenin, and β-catenin+shoxct1 groups. Data are presented as the mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ns, not significant.

Article Snippet: Primary antibodies against the following proteins were used: β-actin (#20536-1-AP, Proteintech, China), OXCT1 (#12175-1-AP, Proteintech, China), β-catenin (#8480, Cell Signaling Technology, USA), PPARα (#66826-1-Ig, Proteintech, China), HMGCS2 (#ab137043, Abcam, USA), BDH1 (#15417-1-AP, Proteintech, China), LEF1 (#14972-1-AP, Proteintech, China), MMP2 (#10373-2-AP, Proteintech, China), Snai1 (13099-1-AP, Proteintech, China), Vimentin(#60330-1-Ig, Proteintech, China), E-cadherin (HY- P81271 , MCE, China), STAT3 (#9139, Cell Signaling Technology, USA), p-STAT3 (#9145, Cell Signaling Technology, USA).

Techniques: Stable Transfection, Infection, Knockdown, Control, Western Blot, Wound Healing Assay, Migration, Injection, Staining

Journal: Molecular Medicine Reports

Article Title: Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air-blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF-κB/IκBα pathway

doi: 10.3892/mmr.2026.13797

Figure Lengend Snippet: Injury of mucus hypersecretion and oxidative damage in all groups. (A) AB-PAS staining images of the airway in all groups (AB-PAS; ×200 magnification and ×400 magnification). Arrows indicate goblet cells. (B) The expression level of MUC5AC and MUC5B in the airway tested using immunohistochemistry (×200 magnification). (C) The expression of IL-6, IL-10, TNF-α, T-SOD, MDA, MMP-9 and TIMP-1 in lung tissue and serum of rats in all groups. The values are expressed as mean ± SE; (n=8). *P<0.05 and **P<0.01 vs. normal group. AB-PAS, Alcian blue-periodic acid-schiff; MUC5AC, mucoprotein-5AC; MUC5B, mucoprotein-5B; T-SOD, total superoxide dismutase; MDA, malondialdehyde; MMP-9, matrix metalloproteinase 9; TIMP-1, tissue inhibitor of metalloproteinases 2; IOD, integrated optical density.

Article Snippet: The secretion of IL-6 (Rat IL-6 ELISA Kit; cat. no. E-EL-R0015; Elabscience Bionovation Inc.), IL-10 (Rat IL-10 ELISA Kit; cat. no. E-EL-R0016; Elabscience Bionovation Inc.), malondialdehyde (MDA) (MDA assay kit; cat. no. A003-1-2; Nanjing Jiancheng Bioengineering Institute), total superoxide dismutase (T-SOD) (SOD assay kit; cat. no. A001-3-2; Nanjing Jiancheng Bioengineering Institute), MMP-9 (Rat MMP-9 ELISA Kit; cat. no. E-EL-R3021; Elabscience Bionovation Inc.) and tissue Inhibitors of metalloproteinase 1 (TIMP-1 ELISA Kit; cat. no. E-EL-R0540; Elabscience Bionovation Inc.) in the lung tissue homogenate was measured using ELISA kits, following the manufacturer's instructions.

Techniques: Staining, Expressing, Immunohistochemistry