Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: The two composite paste compositions analyzed, differing most critically in their weight contents of gelatin: 19.4 and 26.5 wt.%.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques:

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Schematic representation of the major steps in the synthesis of the self-setting HAp/gelatin/silica composite paste.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques:

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: SEM (a) and TEM (b) image of set HAp-gelatin-silica pastes showing the presence of HAp nanoparticles (a) and their encapsulation within an amorphous, polymeric gelatin/silica shell (b).

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques:

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: SEM-derived particle size distribution histogram for HAp nanoparticles comprising the composite HAp-gelatin-silica pastes (a) and DLS-derived average particle size of the composite HAp-gelatin-silica pastes with two different gelatin contents, 19.4 and 26.5 wt.%, suspended in water (b). Data are shown as averages with error bars representing standard deviation.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Derivative Assay, Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Zeta potential vs. pH curves for HAp nanoparticles comprising the composite HAp-gelatin-silica pastes and for the composite HAp-gelatin-silica pastes containing 19.4 and 26.5 wt.% of gelatin. Points of zero charge (PZC) are denoted in the graph. Data are shown as averages with error bars representing standard deviation.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Variation of the initial and the final setting times of HAp-gelatin-silica composite pastes depending on: (a) the content of gelatin in pastes with powder-to-liquid ratio (P/L) of 0.33 set at 37 °C using 2 wt.% NaH2PO4 as the liquid phase; (b) P/L ratio in 60 μl 19.4 wt.% gelatin-containing pastes set at 37 °C and 2 wt.% NaH2PO4 as the liquid phase, along with the maximal hypodermic needle gauge numbers capable of extruding the paste in air for the given P/L ratio ranges; (d), the setting temperature of 60 μl 19.4 wt.% gelatin-containing paste with P/L = 0.5. The logarithmic plot of the final setting time (minutes as units) of 19.4 wt.% gelatin-containing pastes in (b) as a function of P/L ratio is shown in (c), along with the linear fit, the R2 value and the equation derived to correlate the setting time at 37 °C with the P/L ratio. Data are shown as averages with error bars representing standard deviation.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Derivative Assay, Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: SEM images of a set HAp-gelatin-silica composite paste prior (center) and after three weeks of incubation in water (left and right), differing only in the liquid phase used in the paste formation stage: 2.5 wt.% K2HPO4 and 1 wt.% NaCl (left) or 5 wt.% NaH2PO4 (right).

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Incubation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: XRD patterns of a composite HAp-gelatin-silica paste and of its major two individual components: HAp and gelatin. Reflections derived from HAp are marked with Δ.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Derivative Assay

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Timelapse XRD patterns of the composite, vancomycin-loaded HAp-gelatin-silica pastes containing 26.5 wt.% of gelatin in the course of the setting process taking 15 minutes to reach the final setting time. Patterns were taken in the course of 120 min following the initiation of the setting reaction and are plotted with both absolute (a) and relative (b) diffraction intensities.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques:

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Vancomycin release profiles of HAp-gelatin-silica pastes containing different amounts of the gelatin component: 19.4 and 26.5 wt.%. The last two data points for the 26.5 wt.% gelatin system are the result of extrapolation of the preceding portion of the curve. Data are shown as averages with error bars representing standard deviation.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Bacterial population in a liquid inoculation assay of vancomycin-free composite HAp pastes containing 19.4 wt.% gelatin (Sample), vancomycin-loaded composite HAp pastes containing 19.4 wt.% gelatin (Sample + V) and pure vancomycin (V) against (a) S. aureus, (b) S. enteritidis, (c) S. liquefaciens, (d) P. aeruginosa and (e) E. coli. Assays were carried out after days 1, 2, 3, 6 and 7. Data are shown as averages with error bars representing standard deviation. Data points significantly lower in value compared to the untreated control (far left, p < 0.05) are topped with an asterisk. Asterisks topping the lines connecting individual data points from the Sample + V group with the corresponding data points from the V group denote the significantly lower (p < 0.05) values in the former group compared to the latter.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Bacterial population in a liquid inoculation assay of vancomycin-free HAp pastes containing 26.5 wt.% gelatin (Sample), vancomycin-loaded composite HAp pastes containing 26.5 wt.% gelatin (Sample + V) and pure vancomycin (V) against (a) S. enteritidis, and (b) P. aeruginosa. Assays were carried out after days 1, 2, 3, 6 and 7. Data are shown as averages with error bars representing standard deviation. Data points significantly lower in value compared to the untreated control (far left, p < 0.05) are topped with an asterisk. Asterisks topping the lines connecting individual data points from the Sample + V group with the corresponding data points from the V group denote the significantly lower (p < 0.05) values in the former group compared to the latter.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Optical images (a, b) and median lengths (c) of gram-stained S. liquefaciens bacilli having undergone no treatment (a) and having been treated with HAp-gelatin-silica pastes. The destruction of bacteria proceeds in the following steps: (1) filamentation through the formation of dimers and multimers; (2) increase in the filament aspect ratio; (3) extensive aggregation; and (4) formation of necrotic spheroids. Data in (c) are shown as averages with error bars representing standard deviation. The asterisk denotes an extremely statistically significant difference (P < 0.0001).

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Staining, Standard Deviation

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: hMSC viability following the treatment with the vancomycin-loaded composite HAp-gelatin-silica pastes containing different amounts of gelatin: 19.4 and 26.5 wt.%. Bars represent averages, while error bars are standard deviations. No statistically significant difference (p < 0.05) is detected between any of the sample groups.

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques:

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes with Tunable Setting Properties

doi: 10.1039/C7TB01794D

Figure Lengend Snippet: Confocal fluorescent optical images showing hMSCs subjected to no treatment (a) and challenged with either a pure HAp paste (b-c) or a composite HAp-gelatin-silica paste (d-e). Cells were stained for nucleus (blue) and f-actin cytoskeletal microfilaments (red), while the nanomaterial was stained in green. Data in (f) represent a difference in the amount of uptaken material per cell and are shown as averages with error bars representing standard deviation. The asterisk denotes an extremely statistically significant difference (P < 0.001).

Article Snippet: Particle size distribution of the composite HAp-gelatin-silica pastes dispersed in water was analyzed using a Malvern Zetasizer dynamic light scattering analyzer.

Techniques: Staining, Standard Deviation