Journal: Neurotoxicology and teratology

Article Title: Dose-Response Cocaine Pharmacokinetics and Metabolite Profile Following Intravenous Administration and Arterial Sampling in Unanesthetized, Freely Moving Male Rats

doi:

Figure Lengend Snippet: Concentration (mean ± SEM) vs. time curves for cocaine and major (BE) as well as minor metabolites (EME and NC) are shown for the animals that received an IV bolus injection of 3 mg/kg cocaine. The relative ordering of the metabolites indicated that BE was the primary metabolite, with average EME values approximately 1/10 that of BE and average NC values approximately 1/40 that of BE.

Article Snippet: Commercial standard solutions of cocaine metabolites at 1.0 mg/ml in methanol were from Alltech (State College, PA).

Techniques: Concentration Assay, Injection

Journal: Neurotoxicology and teratology

Article Title: Dose-Response Cocaine Pharmacokinetics and Metabolite Profile Following Intravenous Administration and Arterial Sampling in Unanesthetized, Freely Moving Male Rats

doi:

Figure Lengend Snippet: A dose-dependent shift in metabolite profile is illustrated as indexed by the area under the concentration (mean ± SEM) vs. time curve from T0 to T30. Although the AUC for the concentration vs. time function from T0 to T30 for each of the metabolites was similarly ordered for all dose groups (BE > EME > NC), a significant shift in metabolite concentrations was evident as a function of increasing cocaine dose. Whereas BE was increased, both EME and NC were depressed at the 3.0 mg/kg dose.

Article Snippet: Commercial standard solutions of cocaine metabolites at 1.0 mg/ml in methanol were from Alltech (State College, PA).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease

doi: 10.1038/s41598-018-37512-8

Figure Lengend Snippet: Comparison of base peak electropherograms of standard metabolite mixture consisting of 60 metabolites at 50 µM ( A ) and a QC sample of tissue sections ( B ) obtained with the sheathless CE-MS platform. Experimental conditions: BGE, 10% (v/v) acetic acid (pH 2.3); voltage, +20 kV; sample injection, 1 psi × 60 s (1.4% total volume of the capillary, i.e. roughly 9 nL); 91 cm × 30 µm i.d. × 150 µm o.d. fused-silica capillary.

Article Snippet: A standard metabolite mixture comprised of roughly 60 metabolites, with a concentration of 50 μM in water, was obtained from Human Metabolome Technologies (Tokyo, Japan), the so-called “HMT” mixture.

Techniques: Comparison, Injection