Journal: The Journal of experimental biology

Article Title: Adenosine and γ-aminobutyric acid partially regulate metabolic and ventilatory responses of Damaraland mole-rats to acute hypoxia.

doi: 10.1242/jeb.246186

Figure Lengend Snippet: Fig. 3. GABAA receptor antagonism prevents the hypoxic ventilatory response. (A,B,D,E) Summaries of the air convection requirements of O2 (ACRO2; A,B) and CO2 (ACRCO2; D,E) from Damaraland mole-rats exposed to 21% O2, before and after intraperitoneal injections of saline alone (white bars and open symbols), or the GABAA receptor antagonist bicuculline (1 mg kg−1; dissolved in saline; pink bars and closed symbols), and subsequent exposure to acute hypoxia (7 or 5% O2; 1 h). Data are presented as box and whiskers plots of min–max with individual replicates shown from n=7–10 biological replicates per dataset. (C,F) Summaries of the hypoxia-mediated change in ACRO2 (C) and ACRCO2 (F) from animals treated as in A and B. Summary data are means±s.e.m. Different letters indicate significance as determined using a 2-way ANOVA or mixed-effects model with Tukey’s post hoc tests, P<0.05.

Article Snippet: Analysis Ventilatory and metabolic data were collected using LabChart software (ADInstruments, Colorado Springs, CO, USA) and analysed in PowerLab (ADInstruments).

Techniques: Convection, Saline

Journal: The Journal of experimental biology

Article Title: Adenosine and γ-aminobutyric acid partially regulate metabolic and ventilatory responses of Damaraland mole-rats to acute hypoxia.

doi: 10.1242/jeb.246186

Figure Lengend Snippet: Fig. 6. Adenosine receptor antagonism prevents the relative hypoxic ventilatory response. (A,C) Summaries of the air convection requirements of O2 (ACRO2; A) and CO2 (ACRCO2; C) from Damaraland mole-rats exposed to 21% O2, before and after injection of saline (white bars and open symbols) or aminophylline (100 mg kg−1; dissolved in saline; blue bars and closed symbols), and subsequently exposed to acute hypoxia (7% O2 for 1 h). Data are presented as box and whisker plots of min–max with individual replicates shown from n=7–10 biological replicates per dataset. Different letters indicate significance as determined using a two-way ANOVA or mixed-effects model with Tukey’s post hoc tests, P<0.05. (B,D) Summaries of the hypoxia-mediated change in ACRO2 (B) and ACRCO2 (D) from animals treated as in A and C. Summary data are means±s.e.m. Asterisks indicate significant difference from saline controls as determined using paired Student’s t-tests, P<0.05.

Article Snippet: Analysis Ventilatory and metabolic data were collected using LabChart software (ADInstruments, Colorado Springs, CO, USA) and analysed in PowerLab (ADInstruments).

Techniques: Convection, Injection, Saline, Whisker Assay

Journal: Molecular Metabolism

Article Title: Acetate drives ovarian cancer quiescence via ACSS2-mediated acetyl-CoA production

doi: 10.1016/j.molmet.2024.102031

Figure Lengend Snippet: Multi-omics analysis identifies ACSS2 as a candidate regulator of quiescence . (A – B) Schematic representation of RNA-Seq (A) and CRISPR KO screen (B) of serum starved (serum free) Ovcar8 cells. (C) Cross-comparison of RNA-seq and CRISPR KO screen results (cutoffs for RNA-seq: fold-change ≥2, FDR ≤0.1; Cutoff for CRISPR screen: pval ≤0.1). (D) Volcano plot of RNA-seq of serum starved Ovcar8 results showing differential expression of common genes from (C) . (E) ACSS2 protein expression was determined by western blotting in the indicated cells. β-actin was used as a loading control. Western blots shown are representative data from at least 2 independent experiments in each cell line and condition.

Article Snippet: Human pooled metabolic CRISPR KO library (Addgene #110066), pLV-mCherry-hCdt1(1–100)ΔCy (Addgene #193759), and pCDH-EF1-mVenus-p27K − (Addgene #176651) were obtained from Addgene.

Techniques: Biomarker Discovery, RNA Sequencing, CRISPR, Comparison, Quantitative Proteomics, Expressing, Western Blot, Control

Journal: Disease Models & Mechanisms

Article Title: A small-molecule screen identifies that elafibranor links mechanical cues and Irf6-dependent epithelial differentiation

doi: 10.1242/dmm.052649

Figure Lengend Snippet: Carbohydrate metabolism small-molecule screen to identify modulators of irf6 periderm regulation in zebrafish embryos. (A) Schematic of the high-throughput chemical screen in which irf6 maternal-null (m-null) embryos were incubated with each of the compounds in a 384-well format. To visualize enveloping layer (EVL) instability leading to embryo rupture at the onset of epiboly, propidium iodide (PI) was included in the buffer to enable fluorescent readout and quantification using a plate reader. Created in BioRender by Molteni, H. L. (2026). https://BioRender.com/r2ydfea . This figure was sublicensed under CC-BY 4.0 terms. (B) Plot showing average PI fluorescence over early development [4-12 h post-fertilization (hpf)] of wild-type and irf6 m-null embryos exposed to 10 µM elafibranor or DMSO vehicle control. Data are mean±s.e.m. n =48 embryos across three experiments. (C) Heat map of wild-type and irf6 m-null controls (DMSO) and 18 selected compounds that delayed irf6 m-null rupture from plate reader data. Dashed line denotes the time of rupture. All conditions were run in at least three replicates. n =3 embryos. (D) Representative brightfield images of irf6 m-null and wild-type embryos treated with DMSO or 10 µM elafibranor at the following developmental timepoints: sphere, dome, 50% epiboly and bud stage. DMSO-treated irf6 m-null embryos failed to initiate epiboly, rupturing (denoted with an ‘X’) during the sphere stage. Elafibranor delayed rupture time, allowing irf6 m-null embryos to undergo epiboly initiation before rupturing at 50% epiboly. Images taken during mid-rupture are in the black-outlined box to the right of the ‘X’. Wild-type embryos treated with 10 µM elafibranor did not rupture and progressed through epiboly. n =75 embryos across three independent experiments. Scale bar: 200 µm.

Article Snippet: Small molecules were obtained from the Carbohydrate Metabolism Compound Library (Selleck Chem) and stored at −80°C prior to use.

Techniques: High Throughput Screening Assay, Incubation, Fluorescence, Control