Journal: Mikrochimica Acta

Article Title: Detection of SARS-CoV-2 receptor binding domain using fluorescence probe and DNA flowers enabled by rolling circle amplification

doi: 10.1007/s00604-023-05747-6

Figure Lengend Snippet: Evaluation of specificity of the RCA-enabled fluorometric ( a ) and colorimetric assay ( b ). The signal intensity generated from S protein RBD is compared with that from N protein, MERS protein RBD, SARS-Cov-2 B.1.617.2 spike protein RBD, and a mixture of the three proteins. The total protein concentration in each sample was 50 ng/mL

Article Snippet: Recombinant SARS-CoV-2 receptor-binding domain (RBD, source: HEK293-derived SARS-CoV-2 Spike RBD protein (Arg319-Phe541), with a 6x-His tag at C-terminal), recombinant MERS-CoV Spike RBD (Chinese Hamster Ovary-derived MERS-CoV Spike RBD protein (Glu367-Tyr606), with a 6x-His tag at C-terminal), recombinant SARS-CoV-2 B.1.617.2 RBD (source: HEK293-derived SARS-CoV-2 Spike RBD protein (Arg319-Phe541 (Leu452Arg, Thr478Lys), with a 6x-His tag at C-terminal), and recombinant SARS-CoV-2 nucleocapsid (source: Spodoptera frugiperda , Sf 21 (baculovirus)-derived SARS-CoV-2 nucleocapsid (Met1-Ala419), with a 6x-His tag at C-terminal) were purchased from Bio-Techne (Minneapolis, USA).

Techniques: Colorimetric Assay, Generated, Protein Concentration

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: Coronavirus , MERS , MERS , S1-RBD , AFS88936.1 , Insect Cells , Sino Biological , N-(AA383-502)-Fc-C , 40071-V05B.

Techniques: Microarray, Expressing, Construct

Journal: bioRxiv

Article Title: LRRC15 is an inhibitory receptor blocking SARS-CoV-2 spike-mediated entry in trans

doi: 10.1101/2021.11.23.469714

Figure Lengend Snippet: (A) A375 cells were transduced with indicated activating sgRNAs and incubated with SARS-CoV-2 spike S1-Fc fusion protein. Protein binding was measured by flow cytometry. (B) HeLa cells were transduced with indicated activating sgRNAs and incubated with SARS-CoV-2 spike S1-Fc fusion protein. Protein binding was measured by flow cytometry. (C) Dose-dependent binding of SARS-CoV-2 spike protein (Wuhan-Hu-1) to both ACE2 and LRRC15 with a Fc tag was determined by ELISA. Human IgG1 was included as a negative control. Dots indicate means of duplicates. (D) HeLa cells were transduced with indicated activating sgRNAs and incubated with SARS-CoV-2 spike NTD-Fc or RBD-Fc fusion protein. Protein binding was measured by flow cytometry. (E) The binding of the SARS-CoV-2 RBD and NTD to LRRC15 was measured by ELISA.

Article Snippet: Then either rabbit Fc tagged-MERS-CoV spike/RBD protein fragment (Sino biological, #40071-V31B1) or SARS-CoV spike/RBD protein fragment (Sino biological, #40150-V31B2) were 4-fold serially diluted (starting concentration, 16 µg/mL) and added on the plate for 2-hour incubation.

Techniques: Transduction, Incubation, Protein Binding, Flow Cytometry, Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Science Bulletin

Article Title: Binding of the SARS-CoV-2 spike protein to glycans

doi: 10.1016/j.scib.2021.01.010

Figure Lengend Snippet: Schematic representation of CoV S proteins. The diagrams show the domain organization of the S proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV. The sequences of the S1/S2 and S2’ cleavage sites are given on top of each diagram. The arrows indicate the cleave points.

Article Snippet: The recombinant SARS-CoV-2-S2 (686–1213), MERS-CoV-RBD (367–606), MERS-CoV-S1 (1–725), MERS-CoV-S2 (726–1296), and MERS-CoV-S (1-1297) with C-terminal His-tags were purchased from Sino Biological (Beijing, China).

Techniques:

Journal: Science Bulletin

Article Title: Binding of the SARS-CoV-2 spike protein to glycans

doi: 10.1016/j.scib.2021.01.010

Figure Lengend Snippet: The binding of full-length CoV S proteins and their domains to HS microarrays. (a) SARS-CoV-2-RBD binding at the concentration of 2.5 μg/mL, (b) SARS-CoV-2-S1 at the concentration of 2 μg/mL, (c) SARS-CoV-2-S2 at the concentration of 2 μg/mL, (d) SARS-CoV-2-S at the concentration of 2 μg/mL, (e) SARS-CoV-2-trimer at the concentration of 2 μg/mL, (f) SARS-CoV-RBD at the concentration of 2 μg/mL, (g) SARS-CoV-S1 at the concentration of 2 μg/mL, (h) SARS-CoV-2-S at the concentration of 2 μg/mL, (i) MERS-CoV-RBD at the concentration of 4 μg/mL, (j) MERS-CoV-S1 at the concentration of 4 μg/mL, (k) MERS-CoV-S2 at the concentration of 2 μg/mL, (l) MERS-CoV-S at the concentration of 2 μg/mL. The fluorescence intensity was measured at an excitation wavelength of 532 nm. All error bars are standard deviation of more than three replicates.

Article Snippet: The recombinant SARS-CoV-2-S2 (686–1213), MERS-CoV-RBD (367–606), MERS-CoV-S1 (1–725), MERS-CoV-S2 (726–1296), and MERS-CoV-S (1-1297) with C-terminal His-tags were purchased from Sino Biological (Beijing, China).

Techniques: Binding Assay, Concentration Assay, Fluorescence, Standard Deviation

Journal: Science Bulletin

Article Title: Binding of the SARS-CoV-2 spike protein to glycans

doi: 10.1016/j.scib.2021.01.010

Figure Lengend Snippet: SPR sensorgrams of the interactions between a commercially available porcine heparin and immobilized S proteins and their domains. Panels (a−l) show the binding of heparin to SARS-CoV-2-RBD, S1, S2, S, trimer, SARS-CoV-RBD, S1, S, and MERS-CoV-RBD, S1, S2, S, respectively. The name of each protein and its K D value are presented on top of the corresponding sensorgram. RU, resonance unit.

Article Snippet: The recombinant SARS-CoV-2-S2 (686–1213), MERS-CoV-RBD (367–606), MERS-CoV-S1 (1–725), MERS-CoV-S2 (726–1296), and MERS-CoV-S (1-1297) with C-terminal His-tags were purchased from Sino Biological (Beijing, China).

Techniques: Binding Assay

Journal: Science Bulletin

Article Title: Binding of the SARS-CoV-2 spike protein to glycans

doi: 10.1016/j.scib.2021.01.010

Figure Lengend Snippet: SPR sensorgrams of the interactions between fondaparinux and immobilized S proteins and their domains. Panels (a–l) are the binding of fondaparinux to SARS-CoV-2-RBD, S1, S2, S, trimer, SARS-CoV-RBD, S1, S, and MERS-CoV-RBD, S1, S2, S, respectively. The name and K D value of each protein are presented on top of the corresponding sensorgram. RU, resonance unit.

Article Snippet: The recombinant SARS-CoV-2-S2 (686–1213), MERS-CoV-RBD (367–606), MERS-CoV-S1 (1–725), MERS-CoV-S2 (726–1296), and MERS-CoV-S (1-1297) with C-terminal His-tags were purchased from Sino Biological (Beijing, China).

Techniques: Binding Assay

Journal: Microbiology and Immunology

Article Title: Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus

doi: 10.1111/1348-0421.12643

Figure Lengend Snippet: Characterization of MERS‐CoV RBD expressed in E . coli . (a) Mouse RID‐RBD protein was expressed in the soluble fraction of IPTG‐induced E. coli . IN, IPTG induction; M, protein marker; NI, no IPTG induction; P, cell pellets; S, supernatant; T, total lysate. (b) Purified mRID‐RBD and BSA were loaded onto SDS‐polyacrylamide gels in each lane.

Article Snippet: Mouse MAbs against MERS‐CoV S and RBD proteins were produced by ATGen (Seoul, Korea) using a previously reported protocol .

Techniques: Marker, Purification

Journal: ACS Applied Materials & Interfaces

Article Title: Selective Detection and Ultrasensitive Quantification of SARS-CoV-2 IgG Antibodies in Clinical Plasma Samples Using Epitope-Modified Nanoplasmonic Biosensing Platforms

doi: 10.1021/acsami.2c06599

Figure Lengend Snippet: Specificity and cross reactivity test of epitope-functionalized nanoplasmonic biosensing platforms. (A) UV–vis extinction spectra and (B) representative shift in the λ LSPR peak position (Δλ LSPR ) of 75%/25% S14P5/S21P2-functionalized nanoplasmonic biosensors (black line) after incubation in a solution of 100 nM SARS-CoV-2 neutralizing antibody IgG (red line/red bar), 100 nM human IgG (blue line/blue bar), 100 nM IgM (green line/green bar), and 100 nM MERS-CoV IgG (orange line/orange bar) in PBS buffer. Two-tailed unpaired t -tests, **** p < 0.0001. All data points were established by taking the measurement of six individual biosensors.

Article Snippet: SARS-CoV-2 spike protein and MERS-CoV Spike RBD IgG were purchased from R&D Systems, a BioTechne brand.

Techniques: Incubation, Two Tailed Test