Journal: The Chinese journal of physiology

Article Title: A basal level of γ-linolenic acid depletes Ca 2+ stores and induces endoplasmic reticulum and oxidative stresses to cause death of breast cancer BT-474 cells.

doi: 10.4103/cjp.cjp_30_21

Figure Lengend Snippet: Figure 3: Effect of gamma‑linolenic acid on mitochondrial membrane potential. BT‑474 cells were treated with 30 µM gamma‑linolenic acid for different time periods and mitochondrial membrane potential was quantified by JC‐1 fluorescence assay. Red emission/green emission ratio lower than control values indicates depolarization. Results are mean ± standard error of mean from three independent experiments. *Significantly (P < 0.05) different from control.

Article Snippet: Measurement of mitochondrial membrane potential Mitochondrial membrane potential was measured using a Mitochondrial Membrane Potential Assay Kit (#12664; Cell Signaling, Danvers, MA, USA).

Techniques: Membrane, Fluorescence, Control

Journal: The Chinese journal of physiology

Article Title: A basal level of γ-linolenic acid depletes Ca 2+ stores and induces endoplasmic reticulum and oxidative stresses to cause death of breast cancer BT-474 cells.

doi: 10.4103/cjp.cjp_30_21

Figure Lengend Snippet: Figure 4: Effect of gamma‑linolenic acid on mitochondrial Ca2+. BT‑474 cells were treated with 30 µM gamma‑linolenic acid for different time periods and mitochondrial Ca2+ level was quantified by flow cytometry using Rhod 2 as fluorescence probe. Results are mean ± standard error of mean from three independent experiments. *Significantly (P < 0.05) different from control.

Article Snippet: Measurement of mitochondrial membrane potential Mitochondrial membrane potential was measured using a Mitochondrial Membrane Potential Assay Kit (#12664; Cell Signaling, Danvers, MA, USA).

Techniques: Flow Cytometry, Fluorescence, Control

Journal: Life

Article Title: Santin (5,7-Dihydroxy-3,6,4′-Trimetoxy-Flavone) Enhances TRAIL-Mediated Apoptosis in Colon Cancer Cells

doi: 10.3390/life13020592

Figure Lengend Snippet: The effects of TRAIL combined with santin on the mitochondrial membrane potential (ΔΨm) in colon cancer cells. SW480 and SW620 cells were subject to incubation for 48 h with rhsTRAIL (concentration of 25–100 ng/mL) and/or with santin (25–100 μM). The fluorescent microscopic analysis of DePsipher staining was used to assess the ΔΨm loss in cancer cells (*** p < 0.001 compared with control, +++ p < 0.001 compared with santin, ### p < 0.001 compared with TRAIL).

Article Snippet: The mitochondrial membrane potentials were measured by The DePsipher Kit (R and D Systems, Minneapolis, MN) in fluorescence microscopy.

Techniques: Membrane, Incubation, Concentration Assay, Staining, Control

Journal: PLoS ONE

Article Title: Berberine Induces Caspase-Independent Cell Death in Colon Tumor Cells through Activation of Apoptosis-Inducing Factor

doi: 10.1371/journal.pone.0036418

Figure Lengend Snippet: Cells were treated with berberine at indicated concentrations for 18 h (A and B), as described in . Intracellular ROS levels were detected using DHE probe and the amount of fluorescence was measured using flow cytometry. The fold change of the amount of fluorescence was calculated by comparing that in the treated groups to the control group (A). Cells were treated with the JC-1 probe and then analyzed by flow cytometry to measures the fraction of cells with polarized or depolarized mitochondrial membrane (B). Cells were treated with berberine at 50 µM for 18 h in the presence or absence of 5 µM of Tiron, a ROS scavenger. Cell number change (C) and LDH release (D) were detected as described in . * p <0.01 compared to the control group, # p <0.01 compared to the berberine treated group.

Article Snippet: Δ ψ was measured using the Flow Cytometry Mitochondrial Membrane Potential Detection kit (BD™ Mitoscreen) according to the manufacturer’s instructions.

Techniques: Fluorescence, Flow Cytometry