Journal: Oncogene

Article Title: Nuclear DLC1 exerts oncogenic function through association with FOXK1 for cooperative activation of MMP9 expression in melanoma

doi: 10.1038/s41388-020-1274-8

Figure Lengend Snippet: a DLC1 copy-number variation analysis of liver cancer ( n = 372) and melanoma ( n = 363) cases from the TCGA database. Each dot represents data from one patient. b RNA-sequencing analysis of DLC1 mRNA levels in liver cancer ( n = 369) and melanoma ( n = 461) samples compared with normal liver ( n = 160) and skin ( n = 558) tissues, respectively, from the TCGA database. * p < 0.05, by Student’s t test. c , d Immunofluorescence of DLC1 and MELAN A in 45 primary melanoma specimens in a tissue microarray (TMA). White arrowheads indicate melanoma cells with overlapping expression of nuclear DLC1 and cytoplasmic MELAN A. e Percentage of cases with DLC1 + /MELAN A + , DLC1 + /MELAN A − , DLC1 − /MELAN A + , and DLC1 − /MELAN A − expression . Chi-squared test, p = 0.033. f Percentage of cases with nuclear DLC1 (strong) + cytoplasmic and nuclear DLC1 (weak) + cytoplasmic in the total cohort and MELAN A + cohort. g Western blot detection of DLC1, FOXK1. and MMP9 expression levels in normal melanocytes (HEMa-LP) and a panel of melanoma cell lines. GAPDH served as a loading control. The signal intensities of protein bands are shown in arbitrary units after normalization with GAPDH. Scale bar: 100 µM.

Article Snippet: Tissue microarray of malignant melanoma (ME1002b) and metastatic melanoma (BCC38218) were purchased from US Biomax Inc.

Techniques: RNA Sequencing Assay, Immunofluorescence, Microarray, Expressing, Western Blot

Journal: Redox Biology

Article Title: Decoding NADPH oxidase 4 expression in human tumors

doi: 10.1016/j.redox.2017.05.016

Figure Lengend Snippet: Representative examples of NOX4 immunostaining from a broad array of malignant versus normal tissues. IHC staining of BioMax Multiple Organ TMA MC6163 for NOX4 expression was scored utilizing the following system: 0 = negative/no or blush staining, 1+ = weak, 2+ = moderate, and 3+ = strong. The percentage of bladder (A), esophageal (B), and ovarian (C) tissues stained with NOX4 is illustrated graphically (left), with tissue images depicting the staining pattern of NOX4 mAb (47-6), haemotoxylin and eosin (H&E), or normal rabbit isotype control (IgG2) (right). Malignant tissue staining demonstrated a significant NOX4 expression increase relative to the corresponding normal tissues.

Article Snippet: IHC staining of BioMax Multiple Organ TMA MC6163 for NOX4 expression was scored utilizing the following system: 0 = negative/no or blush staining, 1+ = weak, 2+ = moderate, and 3+ = strong.

Techniques: Immunostaining, Immunohistochemistry, Expressing, Staining, Control

Journal: Redox Biology

Article Title: Decoding NADPH oxidase 4 expression in human tumors

doi: 10.1016/j.redox.2017.05.016

Figure Lengend Snippet: Distribution of NOX4 (47-6) immunostaining in melanoma and serous ovarian adenocarcinoma tissues. (A) Melanoma tissues from BioMax TMA MC6163 illustrate membrane (left), perinuclear in a spindle cell melanoma (center), and nuclear (right) IHC staining. Arrows highlight representative sections which define the NOX4 mAb staining distribution. (B) Serous ovarian adenocarcinoma tissue stained with 47-6 mAb demonstrated NOX4 localization in perinuclear regions. An arrow highlights one section of distinct perinuclear staining. All photomicrographs were taken at 40X digital magnification.

Article Snippet: IHC staining of BioMax Multiple Organ TMA MC6163 for NOX4 expression was scored utilizing the following system: 0 = negative/no or blush staining, 1+ = weak, 2+ = moderate, and 3+ = strong.

Techniques: Immunostaining, Membrane, Immunohistochemistry, Staining

Journal: Scientific Reports

Article Title: Myosin X is required for efficient melanoblast migration and melanoma initiation and metastasis

doi: 10.1038/s41598-018-28717-y

Figure Lengend Snippet: Increased Myo10 expression in human melanoma. ( A ) Examples of Myo10 immunostaining in human nevus (left) and melanoma (right) samples. Scale bar is 200 μm. ( B ) Expression of Myo10 was studied in a tissue microarray consisting of primary and metastatic melanomas and nevi. Examples of Myo10 and S100 staining in melanoma and nevus are shown. ( C ) Box-and-whiskers plots demonstrated differences in Myo10 expression in the nevi ( n = 10) and melanoma ( n = 43) samples. Myo10 expression was significantly higher in melanoma lesions than in nevi ( P = 0.0011).

Article Snippet: For tissue microarray analysis, a tissue microarray consisting of melanoma and nevi cohorts was obtained from US Biomax, Inc. (ME1004e).

Techniques: Expressing, Immunostaining, Microarray, Staining