Journal: Current Issues in Molecular Biology

Article Title: Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells

doi: 10.3390/cimb45100537

Figure Lengend Snippet: Expression of AAVR, HSPG1, and HSPG2 receptors for AAV in melanoma cells (A375, G-361, and SK-MEL-1) and normal fibroblasts (CCD-1064Sk). The results are presented as a mean of three independent repeats ± SD. ( A ) Expression levels (2 −dCt ) of receptors for AAV under standard conditions—constitutive levels. Statistically significant differences between CCD-1064Sk and cancer cells are marked with asterisks (**** p < 0.0001). ( B ) Averaged constitutive expressions (2 −dCt ) of receptors for AAV in the examined cells. The expression levels (2 −dCt ) of the three receptors were averaged for each cell line. ( C ) Changes in the expression (2 −ddCt ) of receptors after exposure to hyperthermia. For each receptor, lighter colors indicate expression at 37 °C and darker at 43 °C. Statistically significant differences between 37 °C and 43 °C are marked with asterisks (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: Cell lines of melanoma A375 (CRL-1619), G-361 (CRL-1424), and SK-MEL-1 (HTB-67) from the Melanoma Cancer Cell Panel (TCP-1013) and the cell line of normal fibroblasts CCD-1064Sk were obtained from the ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 2. RT-PCR analysis of HLA-D gene transcription in the four melanoma cell lines and in the BUA cell line. The number of amplification cycles is given for each primer set in Table I. cDNAs either from cells treated for 48 h with 250 U/ml IFN-g (1) or from untreated cells (2) and the PCR products were analyzed on ethidium bromide-stained agarose gels. To assess that equal amounts of cDNA were used, a 16-cycle amplification was performed on the cDNAs with GAPDH primers, and the correspond- ing Dig-labeled PCR products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 1. Cell surface expression of the HLA-DR molecules in the 42/95 and HT144 melanoma cell lines examined by indirect immunoflu- orescence. The cells were either treated with 250 U/ml IFN-g for 48 h or untreated as indicated on the figure (light histograms). The dark histogram corresponds to the isotypic control. The L243 mAb was used to detect the expression of HLA-DR molecules by cytofluorometry.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Expressing, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 4. RT-PCR analysis of CIITA transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line through a 33-cycle amplification procedure. cDNAs were prepared from cells either untreated (2) or treated (1) with 250 U/ml IFN-g for 6 h. The amplification products were detected on ethidium bromide-stained gels. To assess that equal amounts of cDNAs were used, a 16-cycle amplification was performed on the cDNAs with GAPDH-specific primers, and the Dig-labeled amplifica- tion products were stained with CSPD.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter.

doi: 10.4049/jimmunol.167.1.98

Figure Lengend Snippet: FIGURE 5. RT-PCR analysis of IRF-1 transcript expression in the dif- ferent melanoma cell lines and in the BUA cell line. cDNA quantification with GAPDH-specific primers was performed on Dig-labeled amplified products.

Article Snippet: The HT144 (ATCC: HTB-63), 42/95, M74, and A375 (ATCC: CRL1619) human melanoma cell lines were kindly provided by E. Tartour (Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Curie Institute, Paris, France).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Labeling