Journal: Dermatology Research and Practice

Article Title: Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

doi: 10.1155/2011/532139

Figure Lengend Snippet: Piece of amniotic membrane (a); immunohistochemistry for HMB-45 applied to cells cultured on slide. A large number of melanocytes can be observed (b); micrograph of cross-sectioned AM stained with HMB-45 after cell culture (c). Original magnification: (b, c) X100.

Article Snippet: Melanocyte basal medium (MBM, PromoCell, Heidelberg, Germany) without PMA (phorbol 12-myristate 13-acetate) nor bovine pituitary extract, supplemented with basic fibroblast growth factor (1 ng/mL), human recombinant stem cell factor (50 ng/mL), and human recombinant granulocyte/macrophage colony-stimulating factor (10 ng/mL) for 10–14 days, was used for the melanocyte culture.

Techniques: Membrane, Immunohistochemistry, Cell Culture, Staining

Journal: Dermatology Research and Practice

Article Title: Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

doi: 10.1155/2011/532139

Figure Lengend Snippet: Patient 1. Preoperatively (a); the achromic epidermis was removed using CO 2 laser, and AM carrying cultured melanocytes on the basement membrane surface was applied on the denuded area with one layer of Vaseline gauze. The amniotic membranes were fixed with steri-strips and covered with dry gauze and adhesive tape (b); postoperative photography (>90% repigmentation at 16 weeks) (c).

Article Snippet: Melanocyte basal medium (MBM, PromoCell, Heidelberg, Germany) without PMA (phorbol 12-myristate 13-acetate) nor bovine pituitary extract, supplemented with basic fibroblast growth factor (1 ng/mL), human recombinant stem cell factor (50 ng/mL), and human recombinant granulocyte/macrophage colony-stimulating factor (10 ng/mL) for 10–14 days, was used for the melanocyte culture.

Techniques: Cell Culture, Membrane, Adhesive

Journal: EMBO Reports

Article Title: Differential contribution of TFE3 isoforms to cell motility and invasion

doi: 10.1038/s44319-025-00659-3

Figure Lengend Snippet: ( A ) Heatmap of differentially expressed genes in ARPE19 cells infected with either adenovirus expressing recombinant TFE3-L-Myc or TFE3-S-Myc for 30 h. ( B ) Relative quantitative RT-PCR analysis of the mRNA expression of the indicated genes in ARPE19 cells infected with either adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc, or Control (Null) for 30 h. Data are presented as mean ± SD of three independent experiments. ACP5 ( ***(a) P = 0.0001; (ns) not significant (b) P = 0.7598; ****(c) P < 0.0001; ***(d) P = 0.0003; (ns) not significant (e) P = 0.709; ***(f) P = 0.0001), CHI3L1 ( ****(a) P < 0.0001; (ns) not significant (b) P = 0.1499; ****(c) P < 0.0001; ****(d) P < 0.0001; **(e) P = 0.0011; ****(f) P < 0.0001), ITGAX ( ***(a) P = 0.0004; (ns) not significant (b) P = 0.1892; ***(c) P = 0.0003; **(d) P = 0.004; (ns) not significant (e) P = 0.9899; **(f) P = 0.0028), SNHG15 ( ****(a) P < 0.0001; (ns) not significant (b) P = 0.7576; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P = 0.2091; ****(f) P < 0.0001), TFE3 ( ****(a) P < 0.0001; ****(b) P < 0.0001; ****(c) P < 0.0001; *(d) P = 0.0108; (ns) not significant (e) P = 0.1693; (ns) not significant (f) P = 0.2653) (one-way ANOVA followed by Tukey’s multiple comparison post-test). ( C ) Immunoblot analysis of protein lysates from ARPE19 cells infected with the indicated adenovirus for 30 h. Asterisk (*) indicates unspecific band. ( D ) Quantification of protein levels showing CD11c/GAPDH or YKL-40/GAPDH ratios expressed as fold change as shown in ( C ). Data are presented as mean ± SD of three independent experiments. (ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P = 0.9986; ****(d) P < 0.0001 (one-way ANOVA followed by Dunnett’s multiple comparison post-test). ( E ) Immunofluorescence confocal microscopy of ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc or Control for 30 h, showing the cellular distribution of YKL-40 (green). Scale bars: 10 μm. ( F ) Relative quantitative RT-PCR analysis of the mRNA expression of transcription regulator genes ( CITED1 and C/EBPα ) in ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc or Ad-Null for 30 h. Data are presented as mean ± SD of three independent experiments. CITED1 ( ***(a) P < 0.0001; (ns) not significant (b) P = 0.1888; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P > 0.9999; ****(f) P < 0.0001), C/EBPα ( ***(a) P < 0.0001; (ns) not significant (b) P = 0.1888; ****(c) P < 0.0001; ****(d) P < 0.0001; (ns) not significant (e) P > 0.9999; ****(f) P < 0.0001) (one-way ANOVA followed by Tukey’s multiple comparison post-test). ( G ) Immunoblot analysis of protein lysates from ARPE19 cells infected with adenovirus expressing recombinant TFE3-L-Myc, TFE3-S-Myc, TFE3-L/M106A-Myc, or Ad-Null for 30 h. ( H ) Quantification of protein levels showing CITED1/GAPDH, LAMP1/GAPDH, C/EBPα-p42/GAPDH, C/EBPα-p30/GAPDH, and LC3-II/LC3-I ratios expressed as fold change as shown in ( G ). Data are presented as mean ± SD of three independent experiments. CITED1 ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ****(d) P < 0.0001), C/EBPα (p42) ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ***(d) P = 0.0002 ), LAMP1 ((ns) not significant (a) P = 0.956; *(b) P = 0.0127; **(c) P = 0.0023; **(d) P = 0.0044), C/EBPα (p30) ((ns) not significant (a) P > 0.9999; ****(b) P < 0.0001; (ns) not significant (c) P > 0.9999; ***(d) P = 0.0001), LC3 ((ns) not significant (a) P = 0.8551; ****(b) P < 0.0001; ***(c) P = 0.0009; ****(d) P < 0.0001) (one-way ANOVA followed by Dunnett’s multiple comparison post-test). .

Article Snippet: CITED1 Polyclonal antibody , Proteintech , Cat# 26999-1-AP, RRID:AB_2296838.

Techniques: Infection, Expressing, Recombinant, Quantitative RT-PCR, Control, Comparison, Western Blot, Immunofluorescence, Confocal Microscopy

Journal: Experimental cell research

Article Title: Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes

doi: 10.1016/j.yexcr.2019.111690

Figure Lengend Snippet: The top 5 highest represented GO terms of differentially expressed genes (DEGs) in NHEM-c melanocytes treated with melanoma A375 exo versus those with control 0 exo using CATEGORY COMPARE.

Article Snippet: Cell lines and culture reagents Primary human epidermal melanocytes, NHEM-c cells, were purchased from PromoCell (Heidelberg, Germany).

Techniques: Control, Chemotaxis Assay

Journal: Experimental cell research

Article Title: Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes

doi: 10.1016/j.yexcr.2019.111690

Figure Lengend Snippet: The top 5 highest represented KEGG pathways in NHEM-c melanocytes treated with melanoma A375 exo versus those with control 0 exo using CATEGORY COMPARE.

Article Snippet: Cell lines and culture reagents Primary human epidermal melanocytes, NHEM-c cells, were purchased from PromoCell (Heidelberg, Germany).

Techniques: Control

Journal: Experimental cell research

Article Title: Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes

doi: 10.1016/j.yexcr.2019.111690

Figure Lengend Snippet: Western blot to confirm CXCL2 protein expression. Protein extracts (50ug) from NHEM-c cells treated with melanocyte NHEM-c exo or melanoma A375exo were assessed using the antibody against CXCL2. HSC70 was used as a loading control.

Article Snippet: Cell lines and culture reagents Primary human epidermal melanocytes, NHEM-c cells, were purchased from PromoCell (Heidelberg, Germany).

Techniques: Western Blot, Expressing, Control

Journal: Experimental cell research

Article Title: Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes

doi: 10.1016/j.yexcr.2019.111690

Figure Lengend Snippet: Top 5 enriched GO terms of DEGs in NHEM-c melanocytes by melanoma A375exo and lung cancer A549exo versus Control 0 exo using CATEGORY COMPARE.

Article Snippet: Cell lines and culture reagents Primary human epidermal melanocytes, NHEM-c cells, were purchased from PromoCell (Heidelberg, Germany).

Techniques: Control

Journal: Experimental cell research

Article Title: Comparative gene expression analysis in melanocytes driven by tumor cell-derived exosomes

doi: 10.1016/j.yexcr.2019.111690

Figure Lengend Snippet: Top 5 enriched KEGG pathways of DEGs in NHEM-c melanocytes by melanoma A375exo and lung cancer A549exo versus Control 0 exo using CATEGORY COMPARE.

Article Snippet: Cell lines and culture reagents Primary human epidermal melanocytes, NHEM-c cells, were purchased from PromoCell (Heidelberg, Germany).

Techniques: Control