Journal: Photochemistry and Photobiology

Article Title: Opsin3 Downregulation Induces Apoptosis of Human Epidermal Melanocytes via Mitochondrial Pathway

doi: 10.1111/php.13178

Figure Lengend Snippet: OPN expression in normal human epidermal melanocytes (NHMs). (A) Relative expression level of OPNs was analyzed by qPCR. OPN level was normalized with GAPDH level ( n = 8). (B) The cell lysate was analyzed by Western blot using anti‐OPNs antibody and β‐actin. The relative protein level was quantified using Quantity One software ( n = 3). (C) Immunofluorescence of normal human epidermal melanocyte with specific antibodies against OPNs (yellow), and nuclei (blue) under the fluorescence microscope. Scale bar = 20 μm. (D) Immunofluorescence of normal human epidermal melanocyte with specific antibodies against OPN3 (yellow), and nuclei (blue) under the confocal microscope. Scale bar = 20 μm. (E) OPN3 colocalized with melan‐A in human epidermal melanocytes with immunofluorescence double staining method. Photomicrographs were taken with Cell Observer‐Living Cells (Zeiss) and pseudocolored with Axiovision software (Zeiss). Scale bar = 20 μm (400 × magnification).

Article Snippet: NHMs were also double labeled with mouse monoclonal antihuman OPN3 (1:50; MDL, MD6636‐100) and rabbit monoclonal antihuman melan‐A (1:50; Bioss, Beijing, China, bs‐7362R).

Techniques: Expressing, Western Blot, Software, Immunofluorescence, Fluorescence, Microscopy, Double Staining