Journal: Molecules

Article Title: Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity

doi: 10.3390/molecules27030938

Figure Lengend Snippet: Effect of rhizome and fibrous roots of C. dahurica on the expression of p-NF-κB Protein in the liver after oral administration in male and female rats for 90 days. ( a ) p-NF-κB and β-actin protein electrophoresis images of female rats. ( b ) p-NF-κB/β-actin column charts of female rats. ( c ) p-NF-κB and β-actin protein electrophoresis images of male rats. ( d ) p-NF-κB/β-actin column charts of male rats. Data were expressed as means ± SD (n = 3). ** p < 0.01; *** p < 0.001.

Article Snippet: After incubating at room temperature for 1 h, the blocking solution was discarded, and the primary antibodies of Phospho-NF-κB p65 (3033, 1:1000) and β-actin (4970, 1:1000) (Cell Signaling Technology, Danvers, MA, USA) were added to the membranes, which were incubated at 4 °C overnight and then washed with Tris buffered saline (TBS) containing 0.1% Tween-20 (TBST) buffer for 3 × 10 min.

Techniques: Expressing, Protein Electrophoresis