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hsc007 methylcellulose  (R&D Systems)
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stemxvivotm osteogenic  (R&D Systems)
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stemxvivo chondrogenic base media  (R&D Systems)
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mouse embryonic fibroblast conditioned media  (R&D Systems)
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Mouse Embryonic Fibroblast Conditioned Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n 2 max media  (R&D Systems)
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N 2 Max Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human methylcellulose complete media  (R&D Systems)
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R&D Systems human methylcellulose complete media
Human Methylcellulose Complete Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human vascular endothelial cell growth factor a vegf a  (R&D Systems)
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Human Vascular Endothelial Cell Growth Factor A Vegf A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemxvivo serum free tumor sphere medium  (R&D Systems)
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stemxvivo emt inducing media supplement  (R&D Systems)
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media supplement  (R&D Systems)
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R&D Systems media supplement
Media Supplement, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human methylcellulose enriched media  (R&D Systems)
94
R&D Systems human methylcellulose enriched media
CD34 + hematopoietic stem cells (HSCs) isolated from bone marrow samples of MPN patients or mobilized leukopaks from healthy donors were plated on semi-solid <t>methylcellulose</t> containing media. The number of colonies were counted 12-18 days after plating. (A) Absolute number of colonies obtained upon culturing MPN patient (six patients) or healthy donor (four donors) CD34 + HSCs in semi-solid media that were either untreated or pre-treated with 50 nM everolimus (pre-treatment). (B) Absolute number of colonies obtained upon culturing MPN patient (three patients) or healthy donor (three donors) CD34 + HSCs in semi-solid media with or without 50 nM everolimus (continuous treatment).
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CD34 + hematopoietic stem cells (HSCs) isolated from bone marrow samples of MPN patients or mobilized leukopaks from healthy donors were plated on semi-solid methylcellulose containing media. The number of colonies were counted 12-18 days after plating. (A) Absolute number of colonies obtained upon culturing MPN patient (six patients) or healthy donor (four donors) CD34 + HSCs in semi-solid media that were either untreated or pre-treated with 50 nM everolimus (pre-treatment). (B) Absolute number of colonies obtained upon culturing MPN patient (three patients) or healthy donor (three donors) CD34 + HSCs in semi-solid media with or without 50 nM everolimus (continuous treatment).

Journal: bioRxiv

Article Title: Lysosomal Degradation Pathways Target Mutant Calreticulin and the Thrombopoietin Receptor in Myeloproliferative Neoplasms

doi: 10.1101/2023.07.12.548605

Figure Lengend Snippet: CD34 + hematopoietic stem cells (HSCs) isolated from bone marrow samples of MPN patients or mobilized leukopaks from healthy donors were plated on semi-solid methylcellulose containing media. The number of colonies were counted 12-18 days after plating. (A) Absolute number of colonies obtained upon culturing MPN patient (six patients) or healthy donor (four donors) CD34 + HSCs in semi-solid media that were either untreated or pre-treated with 50 nM everolimus (pre-treatment). (B) Absolute number of colonies obtained upon culturing MPN patient (three patients) or healthy donor (three donors) CD34 + HSCs in semi-solid media with or without 50 nM everolimus (continuous treatment).

Article Snippet: We used human methylcellulose enriched media (R&D systems; HSC005) that is optimized for growth of myeloid, erythroid, and mixed lineage (colony forming unit-granulocyte, erythrocyte, monocyte, and megakaryocyte (CFU-GEMM)) progenitor cells.

Techniques: Isolation