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Image Search Results
Journal: Nature Communications
Article Title: The low-density lipoprotein receptor and apolipoprotein E associated with CCHFV particles mediate CCHFV entry into cells
doi: 10.1038/s41467-024-48989-5
Figure Lengend Snippet: a Expression of LDL-R at the surface of Huh-7.5, A549, TE-671, EBL, MDBK, and PHH cells assessed by flow cytometry. b Huh-7.5 cells or PHH were incubated with 4μg/mL of LDL-R antibody (open bars) or IgG goat (dashed bars) for 1 h at 37 °C before transduction with CCHF tecVLP-NanoLuc. N = 4 independent experiments. Two-way ANOVA test with Sidak’s multiple comparisons (αLDL-R vs. IgG: Huh-7.5 p < 0.0001; PHH p < 0.0001). c Same as ( b ) with Huh-7.5, TE-671, A549 cells with harvesting at 48 h post-transduction (p.t.). N = 4 independent experiments. Two-way ANOVA test with Sidak’s multiple comparisons (αLDL-R vs. IgG: Huh-7.5 p < 0.0001; TE671 p < 0.0001; A549 p < 0.0001). d Same as ( c ) with Huh-7.5, EBL, MDBK cells. N = 3 independent experiments. Two-way ANOVA test with Sidak’s multiple comparisons (αLDL-R vs. IgG: Huh-7.5 p < 0.0001; EBL p = 0.8740; MDBK p = 0.0715). Data are represented as means ± SEM.
Article Snippet: Huh-7.5 cells (kind gift from Charles Rice), HEK-293T kidney cells (ATCC CRL-3216), TE-671 cells (ATCC CRL8805), A549 cells (kind gift from P. Boulanger), VeroE6 cells (ATCC CRL-1586), EBL cells (kind gift from Fabienne Archer),
Techniques: Expressing, Flow Cytometry, Incubation, Transduction