Journal: Oncogene

Article Title: X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancer

doi: 10.1038/s41388-026-03740-w

Figure Lengend Snippet: A , B Xenograft assays showing subcutaneous tumor growth of MCF-7 ( A ) or MDA-MB-231 ( B ) breast cancer cells ectopically expressing dox-inducible FLAG-GFP or XCP-FLAG ( left ). Tumor weight at end of experiment is shown ( right ). Animals injected with MCF-7 cells had E2-pellet implantations at the back of the neck to promote growth of MCF-7 cells in vivo. All animals were fed doxycycline in their chow. Each point represents the mean ± SEM. For MCF-7, GFP n = 7 and XCP n = 7; For MDA-MB-231, GFP n = 8 and XCP n = 8. Significance was calculated using unpaired t-test. C , D Expression of FLAG-GFP or XCP-FLAG in tumor tissue was validated from MCF-7 ( C ) or MDA-MB-231 ( D ) xenograft tumors by Western blot ( left ) and RT-qPCR ( right ). For Western blot, 3 representative tumors from each group are shown. β-tubulin was used as a loading control. For RT-qPCR, the average of all tumors from each group is shown. RNA levels were quantified by normalizing to housekeeping gene RPL19 mRNA. Each bar represents the mean + SEM. For MCF-7, GFP n = 7 and XCP n = 7; For MDA-MB-231, GFP n = 8 and XCP n = 8. [See also Fig. ].

Article Snippet: MCF-7 (ATCC; RRD:CVCL_0031) cells were kindly provided by Benita S. Katzenellenbogen (University of Illinois, Urbana-Champaign, Champaign, IL) and MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC; RRID:CVCL_0062).

Techniques: Expressing, Injection, In Vivo, Western Blot, Quantitative RT-PCR, Control

Journal: Oncogene

Article Title: X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancer

doi: 10.1038/s41388-026-03740-w

Figure Lengend Snippet: A , B Heatmaps ( left ) and Gene Ontology analysis ( right ) showing XCP-mediated gene expression changes in MCF-7 ( A ) and MDA-MB-231 ( B ) xenograft tumors. C , D Box plots showing gene set analysis of XCP-induced genes and their expression levels stratified into breast cancer subtypes using PAM50 gene set analysis ( left ) and stratified by ER status ( right ) in MCF-7 ( C ) and MDA-MB-231 ( D ) xenograft tumors. Observed differences are significant as determined by an ANOVA comparison of the means ( P value < 0.00001). [See also Fig. ].

Article Snippet: MCF-7 (ATCC; RRD:CVCL_0031) cells were kindly provided by Benita S. Katzenellenbogen (University of Illinois, Urbana-Champaign, Champaign, IL) and MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC; RRID:CVCL_0062).

Techniques: Gene Expression, Expressing, Comparison

Journal: medRxiv

Article Title: The miR-362-3p/ BCLAF1 axis regulates cisplatin sensitivity and metastatic progression in triple-negative breast cancer

doi: 10.64898/2026.03.09.26347941

Figure Lengend Snippet: A-C. Kaplan-Meier survival analysis of mice bearing MDA-MB-231 ( A ), CAL-51 ( B ), or MDA-MB-436 ( C ) tumors treated with cisplatin. Median survival was extended by miR-362-3p overexpression (OE) and shortened by miR-362-3p-inhibition compared to respective controls. D. Representative gross morphology of livers at necropsy; white arrows indicate macroscopic metastatic nodules in non-targeting controls (NTC) vs. miR-362-3p-OE. E. Quantitative of metastatic burden from H&E stained liver sections in MDA-MB-231 models. F. qRT-PCR validation of miR-362-3p levels in endpoint xenograft tumors, confirming sustained OE or inhibition throughout the study. Data represent mean±SD. *, ** and *** represent p <0.05, p <0.01, and p <0.001, respectively. Empty vector, EV.

Article Snippet: MDA-MB-231, MDA-MB-436, MDA-MB-468, and HCC1937 were acquired from the American Type Culture Collection (ATCC; Manassas, VA), CA51 (CVCL_1110) was obtained from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany) and SUM149 which was a gift from Dr. Christina Gewinner, Division of Signal Transduction, BIDMC.

Techniques: Over Expression, Inhibition, Staining, Quantitative RT-PCR, Biomarker Discovery, Plasmid Preparation