Journal: Nanomaterials

Article Title: Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery

doi: 10.3390/nano10122334

Figure Lengend Snippet: Cytotoxicity and cellular uptake studies of FITC-stapled peptides ( a ) Cytotoxicity study in human breast adenocarcinoma MDA-MB-231 cells incubated with increasing concentrations (from 0 to 20 µM) of the stapled peptides for 72 h. ( b ) Uptake of FITC-stapled peptides at 5 µM final concentration in MDA-MB-231 cells after 4 h of incubation represented as relative mean fluorescence. The values are normalized with respect to penetratin. Results are presented as means ± standard deviations of three independent experiments performed in triplicate.

Article Snippet: MDA-MB-231-Luc-RFP stable cell line was obtained from AMSBIO (SC041, Abingdon, UK).

Techniques: Incubation, Concentration Assay, Fluorescence

Journal: Nanomaterials

Article Title: Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery

doi: 10.3390/nano10122334

Figure Lengend Snippet: Luciferase activity assay showing the transfection of a 21-mer small interfering RNAs (siRNAs) targeting the expression of luciferase inside MDA-MB-231-Luc-RFP cells. The experiments were carried out with increasing amounts of siLuc (from 50 to 200 nM) complexed with the stapled peptides. The corresponding concentrations of the peptides are from 1.05 µM to 4.2 µM for JMV6580, from 1.4 µM to 5.6 µM for JMV6583 and from 5.25 µM to 21 µM for JMV6582. For lipofectamine transfection, the siRNA concentration used is 50 nM. In parallel, cell viability was measured for each condition. Results are expressed as mean ± standard deviation (n = 3). Statistically different, the level of significance was defined ** p < 0.01, and *** p < 0.001.

Article Snippet: MDA-MB-231-Luc-RFP stable cell line was obtained from AMSBIO (SC041, Abingdon, UK).

Techniques: Luciferase, Activity Assay, Transfection, Expressing, Concentration Assay, Standard Deviation