Journal: PLoS ONE

Article Title: GCN5 Is a Positive Regulator of Origins of DNA Replication in Saccharomyces cerevisiae

doi: 10.1371/journal.pone.0008964

Figure Lengend Snippet: Yeast strains used in this study.

Article Snippet: Cross-linked chromatin was sheared, extracted and immunoprecipitated with a mixture of anti-MCM2 and anti-MCM5 antibodies (Santa Cruz).

Techniques:

Journal: PLoS ONE

Article Title: GCN5 Is a Positive Regulator of Origins of DNA Replication in Saccharomyces cerevisiae

doi: 10.1371/journal.pone.0008964

Figure Lengend Snippet: High dosage of GCN5 and SPT8 partially suppress the Ts − phenotypes of replication factor mutants.

Article Snippet: Cross-linked chromatin was sheared, extracted and immunoprecipitated with a mixture of anti-MCM2 and anti-MCM5 antibodies (Santa Cruz).

Techniques: Control

Journal: PLoS ONE

Article Title: GCN5 Is a Positive Regulator of Origins of DNA Replication in Saccharomyces cerevisiae

doi: 10.1371/journal.pone.0008964

Figure Lengend Snippet: W303 , mcm5-461, orc2-1 and orc5-1 cells carrying pARS1wt ( ARS1, CEN4, URA3 ) were transformed with pGBKT7 ( 2 µm/TRP1 ) or pGBKT7- GCN5 . After selection on SC-trp-ura plates three colonies were transferred for 20 generations in SC-trp and then plated on SC-trp and SC-trp-ura, respectively. The proportion of cells with URA+ phenotype was estimated and the average loss per generation of pARS1wt was calculated (%) as in and plotted. A typical outcome of one of two independent experiments is shown.

Article Snippet: Cross-linked chromatin was sheared, extracted and immunoprecipitated with a mixture of anti-MCM2 and anti-MCM5 antibodies (Santa Cruz).

Techniques: Transformation Assay, Selection

Journal: PLoS ONE

Article Title: GCN5 Is a Positive Regulator of Origins of DNA Replication in Saccharomyces cerevisiae

doi: 10.1371/journal.pone.0008964

Figure Lengend Snippet: Wild type (BY4742) and Δgcn5 cells were arrested in Mitosis by12 µg/ml Nocodazole for 3.5 hours and released in YPD. Wild type cells were harvested at 0, 20 and 60 min past Nocodazole release. Δgcn5 cells were harvested at 0, 20 and 60 min past Nocodazole release. Progression through the cell cycle as monitored by Propidium Iodide staining/FACS is shown on the top. Crosslinked chromatin was isolated and immunoprecipitated with anti-MCM2 and anti-MCM5 antibodies. The ACS fragments of ARS1 and ARS305 and a fragment from ACT1 were amplified by PCR (triplicate) and quantified. The per cent of signal from each fragment in the anti-MYC immunoprecipitate versus Load was calculated and plotted. A typical outcome of one of two independent experiments is shown.

Article Snippet: Cross-linked chromatin was sheared, extracted and immunoprecipitated with a mixture of anti-MCM2 and anti-MCM5 antibodies (Santa Cruz).

Techniques: Staining, Isolation, Immunoprecipitation, Amplification

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.

doi: 10.1210/jc.2004-2459

Figure Lengend Snippet: FIG. 1. Representative examples of PTC and ATC samples immunostained with PCNA, MCM5, and MCM7 antibodies. The primary antibody was omitted for a negative control (data not shown).

Article Snippet: The slides were incubated overnight with mouse monoclonal antibodies against MCM5 (MCA 1860; Serotec, Oxford, UK), MCM7 (141.2, sc-9966; Santa Cruz Biotechnology, Santa Cruz, CA), or proliferating cell nuclear antigen (PCNA) (MAB424; Chemicon, Temecula, CA).

Techniques: Negative Control

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.

doi: 10.1210/jc.2004-2459

Figure Lengend Snippet: FIG. 3. A, Increasing amounts (10, 20, and 50 g) of protein extracts from the indicated cell lines were immunoblotted with anti-MCM7, anti-MCM5, and antitubulin (Tub) antibodies. B, The indicated pri- mary cell cultures were treated with cycloheximide (CHX) for differ- ent time points. Lysates (50 g) were run on SDS-PAGE, and MCMs expression levels were determined by immunoblot. C, MCM mRNA levels were determined by Northern blot. 28S and 18S RNA levels were used for normalization. The results are representative of at least three independent assays. D, Top, The indicated cell lines were tran- siently transfected with pGL3-MCM7LUC plasmid or the empty vec- tor; average SD levels of luciferase activity in three independent experiments are reported. Bottom, ARO cells were cotransfected with pGL3-MCM7LUC and the indicated plasmids. Relative luciferase activity is reported. Average SD results of three independent assays are reported. wt, Wild type.

Article Snippet: The slides were incubated overnight with mouse monoclonal antibodies against MCM5 (MCA 1860; Serotec, Oxford, UK), MCM7 (141.2, sc-9966; Santa Cruz Biotechnology, Santa Cruz, CA), or proliferating cell nuclear antigen (PCNA) (MAB424; Chemicon, Temecula, CA).

Techniques: SDS Page, Expressing, Western Blot, Northern Blot, Transfection, Plasmid Preparation, Luciferase, Activity Assay

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Expression status of MCM2, MCM5 and MCM6 in NSCLC tissue samples Representative immunohistochemical microphotographs of MCM2 (a,b,c), MCM5 (d,e,f) and MCM6 (g,h,i) with high (positive) and low (negative) expression in NSCLC and their adjacent non-malignant tissues. The subtypes are SCCs (b, e, h) and ADCs (c, f, i). Bar = 100μm.

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Relationship Between Protein Overexpression and Clinicopathologic Parameters

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques: Over Expression

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Correlation of MCM2, MCM5 and MCM6 LIs with NSCLC patients' overall survival (OS) Kaplan-Meier curves showing the association between LIs of MCM2 (A), MCM5 (B), MCM6 (C) and OS in different stages and in different histological tumor types in tissue samples. All the P values are shown in the graph, by log-rank test.

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques:

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Univariate and multivariate analysis of survival in patients with squamous cell carcinoma

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques:

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Overexpression of MCM2, MCM5 and MCM6 mRNAs in NSCLC samples Transcript levels of MCM2, MCM5 and MCM6 in non-tumor tissues (N) and NSCLC tissues (SCCs or ADCs) from TCGA_LUSC (N=553) and TCGA_LUAD (N=571) datasets. Data are represented as mean±SD. All P values were calculated by t-test analysis, *** P <0.001.

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques: Over Expression

Journal: Journal of Cancer

Article Title: MCMs expression in lung cancer: implication of prognostic significance

doi: 10.7150/jca.20777

Figure Lengend Snippet: Correlation of MCM2, MCM5 and MCM6 mRNAs with patients' overall survival (OS) in TCGA database. The survival analysis stratified by MCM2, MCM5 and MCM6 expression levels in datasets LUAD - TCGA - Lung adenocarcinoma June 2016 (N=475) and LUSC - TCGA - Lung squamous cell carcinoma June 2016 (N=175) were analyzed using the Survexpress online platforms. The Log-Rank P values are shown in the graph.

Article Snippet: The slides were blocked with 10% normal goat serum for 30 min at 37°C and washed and incubated overnight at 4°C with mouse monoclonal antibody against MCM2 (1:200; 10513-1-AP, Proteintech Group, Inc. Chicago, IL, USA), MCM5 (1:200; 11703-1-AP, Proteintech Group, Inc. Chicago, IL, USA), or MCM6 (1:200; 13347-2-AP, Proteintech Group, Inc. Chicago, IL, USA).

Techniques: Expressing

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Glucagon-Like Peptide-2 Stimulates S-Phase Entry of Intestinal Lgr5+ Stem Cells

doi: 10.1016/j.jcmgh.2022.02.011

Figure Lengend Snippet: Acute hGly2–GLP-2 treatment increases the expression of transcripts involved in G1/S-phase transition. Jejunal mucosal isolates from the mice described in Figure 1 were analyzed by reverse-transcription quantitative polymerase chain reaction for the expression of the following: ( A ) Mcm2 , ( B ) Mcm3 , ( C ) Mcm4 , ( D ) Mcm5 , ( E ) Mcm6 , ( F ) Mcm7 , ( G ) Cdk2 , ( H ) Ccnd1 , ( I ) s100a6 , and ( J ) Igf1 . N = 6–7 for all panels. ∗ P < .05. mRNA, messenger RNA; rRNA, ribosomal RNA.

Article Snippet: Mcm5 , Mm00484840_m1.

Techniques: Expressing, Sublimation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Glucagon-Like Peptide-2 Stimulates S-Phase Entry of Intestinal Lgr5+ Stem Cells

doi: 10.1016/j.jcmgh.2022.02.011

Figure Lengend Snippet: Acute GLP-2 3–33 treatment decreases the expression of transcripts involved in G1/S-phase transition. Jejunal mucosal isolates from the mice described in Figure 5 were analyzed by reverse-transcription quantitative polymerase chain reaction for the expression of the following: ( A ) Mcm2 , ( B ) Mcm3 , ( C ) Mcm4 , ( D ) Mcm5 , ( E ) Mcm6 , ( F ) Mcm7 , ( G ) Cdk2 , ( H ) Ccnd1 , ( I ) s100a6 , and ( J ) Igf1 . N = 5 for all panels. ∗ P < .05. mRNA, messenger RNA; rRNA, ribosomal RNA.

Article Snippet: Mcm5 , Mm00484840_m1.

Techniques: Expressing, Sublimation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Glucagon-Like Peptide-2 Stimulates S-Phase Entry of Intestinal Lgr5+ Stem Cells

doi: 10.1016/j.jcmgh.2022.02.011

Figure Lengend Snippet: Quantitative Polymerase Chain Reaction TaqMan Primers (ThermoFisher Scientific, Mississauga, Canada)

Article Snippet: Mcm5 , Mm00484840_m1.

Techniques: Real-time Polymerase Chain Reaction

Journal: Cell Cycle

Article Title: Cell cycle regulation of embryonic stem cells and mouse embryonic fibroblasts lacking functional Pax7

doi: 10.1080/15384101.2016.1231260

Figure Lengend Snippet: Analysis of factors involved in regulation of cell cycle in both Pax7ko and Pax7wt ESCs and MEFs. (A). RT-qPCR analysis of Ccnd1, Ccne1, Ccna2, Ccnb2, Cdkn1a, Cdkn1b, Cdkn1c, Plk1, Cdc34 , and Mcm5 transcript levels in undifferentiated ESCs, EBs at day 2, 7 and EB outgrowths at day 21 of differentiation, as well as asynchronously dividing, quiescent and synchronously cycling MEFs. Data are shown as CT values, which were normalized against those of Actb mRNA level. Data are represented as the percentage of expression observed in mouse embryos at day 13.5 of development. For each genotype 2 ESC and 3 MEF lines were analyzed, graphs represent the mean values. P-values: *<0.05, ** <0.01, *** <0.001. (B). Western blotting analysis of cyclin E1 levels in ESCs, EBs at day 2 and 5, and EB outgrowths at day 14 of differentiation. Probing with anti-Hsp90 antibody was used as a loading control. Graph represents optical density of cyclin E bands compared to density of Hsp90 bands (optical density of Hsp90 was taken as 100 arbitrary units).

Article Snippet: Next, qPCR analysis was performed using LightCycler 96 instrument (Roche), TaqMan Gene Expression Master Mix (Life Technologies), and specific TaqMan probes: Mm00432359_m1 ( Ccnd1 ), Mm00432367_m1 ( Ccne1 ), Mm00438066_m1 ( Ccna2 ), Mm01171453_m1 ( Ccnb2 ), Mm01243769_m1 ( Mcm5 ), Mm00775040_gH ( Cdc34 ), Mm00440924_g1 ( Plk1 ), Mm04205640_g1 ( Cdkn1a ), Mm00438168_m1 ( Cdkn1b ), Mm01272135_g1 ( Cdkn1c ), Mm01205647_g1 ( Actb ).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control

Journal: Journal of Neuroinflammation

Article Title: Glial remodeling enhances short-term memory performance in Wistar rats

doi: 10.1186/s12974-020-1729-4

Figure Lengend Snippet: TaqMan probe details (Life Technologies) used for qrt-PCR

Article Snippet: Mcm5 , NM_001106170.1 , Rn01536832_m1 , 59.

Techniques: Sequencing, TaqMan Assay