mcd45 vioblue Search Results


anti mouse cd45 antibody vioblue  (Miltenyi Biotec)
96
Miltenyi Biotec anti mouse cd45 antibody vioblue
Figure 4. Cellular binding and internalization assay using mouse NPC. (a) Cellular dot plot after staining with CD146 and <t>CD45.</t> X axis (Pacific Blue) and Y axis (FITC) shows CD45 and CD146, respectively. CD146 high/CD45 low, CD146 low/CD45 low, and CD146 low/CD45 high population were named as P1, P2, and P3, respectively. (b) Histogram of the FcγRIIB expression in each population. X axis (APC) shows the fluorescence of Alexa Fluor 647. Red shows staining with anti- FcγRIIB antibody and blue shows unstaining. (c) Concentration-dependent binding of the antibody in P2. The antibody was incubated at 4 °C for 60 min, and fluorescence was detected by FCM. The data were fitted with the receptor-ligand binding equation. (d) Concentration-dependent internalization rate of the antibody in P2. The antibody was incubated at 37 °C for 5 min. The data were fitted with the Michaelis–Menten equation. Each point shows the mean ± SD (n = 3).
Anti Mouse Cd45 Antibody Vioblue, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcd45+vioblue/CD45+Antibody%2C+anti-mouse%2C+REAfinity/pm33863984-235-17-20
Average 96 stars, based on 1 article reviews
anti mouse cd45 antibody vioblue - by Bioz Stars, 2026-11
96/100 stars
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anti mouse cd45 vioblue  (Miltenyi Biotec)
96
Miltenyi Biotec anti mouse cd45 vioblue

Anti Mouse Cd45 Vioblue, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcd45+vioblue/CD45+Antibody%2C+anti-mouse/pmc08122070-57-0-4
Average 96 stars, based on 1 article reviews
anti mouse cd45 vioblue - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
anti mouse cd45  (Miltenyi Biotec)
96
Miltenyi Biotec anti mouse cd45
a – h MPBC graft characterization following FasL treatment. Percentage of annexin V positive a CD3 + and b CD34 + cells. c Percentage of CD3 + and d CD34 + cells per total <t>CD45</t> + population. HSPCs subpopulations; e Immature (CD34 + CD38 low ), f Multipotent progenitors (CD45RA − CD90 − ) and g self-renewing hematopoietic stem cells (CD45RA − CD90 + ). h Colony-forming units (CFU) profile of: erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM) and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). Engraftment, differentiation and CFU potential as detected in the BM of γ-irradiated (2.75 Gy) NSG mice, 4 weeks post transplantation of 1 × 10 5 human CD34 + cells: i human leukocytes (hCD45 + ) j immature hCD34 + CD38 low progenitors and k human leukocytes subpopulations: B (hCD19 + ), Myelo-monocytic (hCD33 + and CD14 + CD16 − ), NK (hCD56 + CD16 − ), HSPCs (CD34 + ) cells and l number of human colony-forming cells in the mice BM. Data presented as ( a – h ) mean+SD or ( i – l ) individual mice and median. ( a – d ) n = 25 or ( e – h ) n = 3 individual MPBCs donations and ( i – l ) show one representative study out of two, n = 7 (MPBC-CD34) and n = 8 (MPBC + FasL-CD34) female mice per group transplanted with cells from one individual MPBC donation. Statistical analysis preformed using ( a – d ) ANOVA parametric test, ( e – h ) paired T-test or ( i – l ) Mann–Whitney test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Anti Mouse Cd45, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcd45+vioblue/CD45+Antibody%2C+anti-human%2C+REAfinity/pmc07329633-41-5-65
Average 96 stars, based on 1 article reviews
anti mouse cd45 - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier

Image Search Results


Figure 4. Cellular binding and internalization assay using mouse NPC. (a) Cellular dot plot after staining with CD146 and CD45. X axis (Pacific Blue) and Y axis (FITC) shows CD45 and CD146, respectively. CD146 high/CD45 low, CD146 low/CD45 low, and CD146 low/CD45 high population were named as P1, P2, and P3, respectively. (b) Histogram of the FcγRIIB expression in each population. X axis (APC) shows the fluorescence of Alexa Fluor 647. Red shows staining with anti- FcγRIIB antibody and blue shows unstaining. (c) Concentration-dependent binding of the antibody in P2. The antibody was incubated at 4 °C for 60 min, and fluorescence was detected by FCM. The data were fitted with the receptor-ligand binding equation. (d) Concentration-dependent internalization rate of the antibody in P2. The antibody was incubated at 37 °C for 5 min. The data were fitted with the Michaelis–Menten equation. Each point shows the mean ± SD (n = 3).

Journal: Scientific reports

Article Title: A cell based assay for evaluating binding and uptake of an antibody using hepatic nonparenchymal cells.

doi: 10.1038/s41598-021-87912-6

Figure Lengend Snippet: Figure 4. Cellular binding and internalization assay using mouse NPC. (a) Cellular dot plot after staining with CD146 and CD45. X axis (Pacific Blue) and Y axis (FITC) shows CD45 and CD146, respectively. CD146 high/CD45 low, CD146 low/CD45 low, and CD146 low/CD45 high population were named as P1, P2, and P3, respectively. (b) Histogram of the FcγRIIB expression in each population. X axis (APC) shows the fluorescence of Alexa Fluor 647. Red shows staining with anti- FcγRIIB antibody and blue shows unstaining. (c) Concentration-dependent binding of the antibody in P2. The antibody was incubated at 4 °C for 60 min, and fluorescence was detected by FCM. The data were fitted with the receptor-ligand binding equation. (d) Concentration-dependent internalization rate of the antibody in P2. The antibody was incubated at 37 °C for 5 min. The data were fitted with the Michaelis–Menten equation. Each point shows the mean ± SD (n = 3).

Article Snippet: After the reaction, the cells were washed and stained with an anti-mouse CD146 antibody-FITC (Miltenyi, 130-102-230) and anti-mouse CD45 antibody-VioBlue (Miltenyi, 130-110-664).

Techniques: Binding Assay, Staining, Expressing, Fluorescence, Concentration Assay, Incubation, Ligand Binding Assay

Figure 5. Cellular binding and internalization assay using monkey NPC. (a) Cellular dot plot after staining with CD31 and CD45. X axis (Pacific Blue) and Y axis (APC) shows CD31 and CD45, respectively. CD31 low/CD45 high, CD31 mid-low/CD45 mid-high, CD31 mid-low/CD45 high, CD31 high/CD45 high, CD31 mid-high/CD45 mid-low, and CD31 low/CD45 low population were named as P1, P2, P3, P4, P5, and P6, respectively. (b) Histogram of the FcγRIIB expression in each population. X axis (FITC) shows the fluorescence of Alexa Fluor 488. Red shows staining with anti- FcγRIIB antibody and blue shows unstaining. (c) Concentration-dependent binding of the antibody in P2 cells. The antibody was incubated at 4 °C for 60 min, and fluorescence was detected by FCM. The data were fitted with the receptor-ligand binding equation. (d) Concentration-dependent internalization rate of the antibody in P2 cells. The antibody was incubated at 37 °C for 10 min. The data were fitted with the Michaelis–Menten equation. Each point shows the mean ± SD (n = 3).

Journal: Scientific reports

Article Title: A cell based assay for evaluating binding and uptake of an antibody using hepatic nonparenchymal cells.

doi: 10.1038/s41598-021-87912-6

Figure Lengend Snippet: Figure 5. Cellular binding and internalization assay using monkey NPC. (a) Cellular dot plot after staining with CD31 and CD45. X axis (Pacific Blue) and Y axis (APC) shows CD31 and CD45, respectively. CD31 low/CD45 high, CD31 mid-low/CD45 mid-high, CD31 mid-low/CD45 high, CD31 high/CD45 high, CD31 mid-high/CD45 mid-low, and CD31 low/CD45 low population were named as P1, P2, P3, P4, P5, and P6, respectively. (b) Histogram of the FcγRIIB expression in each population. X axis (FITC) shows the fluorescence of Alexa Fluor 488. Red shows staining with anti- FcγRIIB antibody and blue shows unstaining. (c) Concentration-dependent binding of the antibody in P2 cells. The antibody was incubated at 4 °C for 60 min, and fluorescence was detected by FCM. The data were fitted with the receptor-ligand binding equation. (d) Concentration-dependent internalization rate of the antibody in P2 cells. The antibody was incubated at 37 °C for 10 min. The data were fitted with the Michaelis–Menten equation. Each point shows the mean ± SD (n = 3).

Article Snippet: After the reaction, the cells were washed and stained with an anti-mouse CD146 antibody-FITC (Miltenyi, 130-102-230) and anti-mouse CD45 antibody-VioBlue (Miltenyi, 130-110-664).

Techniques: Binding Assay, Staining, Expressing, Fluorescence, Concentration Assay, Incubation, Ligand Binding Assay

Journal: Cell host & microbe

Article Title: Bacteria Induce Skin Regeneration via IL-1β Signaling

doi: 10.1016/j.chom.2021.03.003

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD45 Vioblue , Miltenyi Biotec , 130118953 Clone:30F11.

Techniques:

a – h MPBC graft characterization following FasL treatment. Percentage of annexin V positive a CD3 + and b CD34 + cells. c Percentage of CD3 + and d CD34 + cells per total CD45 + population. HSPCs subpopulations; e Immature (CD34 + CD38 low ), f Multipotent progenitors (CD45RA − CD90 − ) and g self-renewing hematopoietic stem cells (CD45RA − CD90 + ). h Colony-forming units (CFU) profile of: erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM) and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). Engraftment, differentiation and CFU potential as detected in the BM of γ-irradiated (2.75 Gy) NSG mice, 4 weeks post transplantation of 1 × 10 5 human CD34 + cells: i human leukocytes (hCD45 + ) j immature hCD34 + CD38 low progenitors and k human leukocytes subpopulations: B (hCD19 + ), Myelo-monocytic (hCD33 + and CD14 + CD16 − ), NK (hCD56 + CD16 − ), HSPCs (CD34 + ) cells and l number of human colony-forming cells in the mice BM. Data presented as ( a – h ) mean+SD or ( i – l ) individual mice and median. ( a – d ) n = 25 or ( e – h ) n = 3 individual MPBCs donations and ( i – l ) show one representative study out of two, n = 7 (MPBC-CD34) and n = 8 (MPBC + FasL-CD34) female mice per group transplanted with cells from one individual MPBC donation. Statistical analysis preformed using ( a – d ) ANOVA parametric test, ( e – h ) paired T-test or ( i – l ) Mann–Whitney test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Bone Marrow Transplantation

Article Title: Brief ex vivo Fas-ligand incubation attenuates GvHD without compromising stem cell graft performance

doi: 10.1038/s41409-020-0941-2

Figure Lengend Snippet: a – h MPBC graft characterization following FasL treatment. Percentage of annexin V positive a CD3 + and b CD34 + cells. c Percentage of CD3 + and d CD34 + cells per total CD45 + population. HSPCs subpopulations; e Immature (CD34 + CD38 low ), f Multipotent progenitors (CD45RA − CD90 − ) and g self-renewing hematopoietic stem cells (CD45RA − CD90 + ). h Colony-forming units (CFU) profile of: erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM) and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). Engraftment, differentiation and CFU potential as detected in the BM of γ-irradiated (2.75 Gy) NSG mice, 4 weeks post transplantation of 1 × 10 5 human CD34 + cells: i human leukocytes (hCD45 + ) j immature hCD34 + CD38 low progenitors and k human leukocytes subpopulations: B (hCD19 + ), Myelo-monocytic (hCD33 + and CD14 + CD16 − ), NK (hCD56 + CD16 − ), HSPCs (CD34 + ) cells and l number of human colony-forming cells in the mice BM. Data presented as ( a – h ) mean+SD or ( i – l ) individual mice and median. ( a – d ) n = 25 or ( e – h ) n = 3 individual MPBCs donations and ( i – l ) show one representative study out of two, n = 7 (MPBC-CD34) and n = 8 (MPBC + FasL-CD34) female mice per group transplanted with cells from one individual MPBC donation. Statistical analysis preformed using ( a – d ) ANOVA parametric test, ( e – h ) paired T-test or ( i – l ) Mann–Whitney test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following antibodies were used: anti- mouse CD45 (130-102-412), human CD45 (130-098-143, 130-098-151, 130-098-141, 130-098-148, 130-104-566, 130-110-637), CD34 (130-081-002, 130-090-954), CD90 (130-099-289), CD3 (130-109-460, 130-109-466), CD4 (130-110-680), CD8 (130-109-454), CCR7 (130-108-309), CD45RA (130-108-784, 130-110-637), LFA1 (130-105-437), CD95 (130-104-232), CXCR3 (130-101-378), CCR6 (130-100-377), CD25 (130-109-021), CD33 (130-111-021), CD19 (130-113-642, 130-110-249), CD27 (130-099-499), CD38 (130-108-862), HLA-DR (130-104-825), IL17 (130-100-077), IFN-γ (130-097-944) and FoxP3 (130-098-119) all purchased from Miltenyi Biotech (Bergisch Gladbach, Germany); matched isotype controls were used as negative control.

Techniques: Irradiation, Transplantation Assay, MANN-WHITNEY