mcc13 Search Results


bloxall endogenous peroxidase and alkaline phosphatase blocking solution  (Vector Laboratories)
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Vector Laboratories bloxall endogenous peroxidase and alkaline phosphatase blocking solution
Bloxall Endogenous Peroxidase And Alkaline Phosphatase Blocking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcc13  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures mcc13
Growth of MCPyV + and MCPyV − MCC cell lines under different experimental conditions in OERCs.
Mcc13, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcc13 cell line  (Seradigm)
90
Seradigm mcc13 cell line
Growth of MCPyV + and MCPyV − MCC cell lines under different experimental conditions in OERCs.
Mcc13 Cell Line, supplied by Seradigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcc-13-bpa-0067  (Millennium Challenge Corporation)
90
Millennium Challenge Corporation mcc-13-bpa-0067
Growth of MCPyV + and MCPyV − MCC cell lines under different experimental conditions in OERCs.
Mcc 13 Bpa 0067, supplied by Millennium Challenge Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Growth of MCPyV + and MCPyV − MCC cell lines under different experimental conditions in OERCs.

Journal: Cancers

Article Title: Organotypic Epithelial Raft Cultures as a Three-Dimensional In Vitro Model of Merkel Cell Carcinoma

doi: 10.3390/cancers14041091

Figure Lengend Snippet: Growth of MCPyV + and MCPyV − MCC cell lines under different experimental conditions in OERCs.

Article Snippet: The other MCC cell lines were obtained from the European Collection of Authenticated Cell Cultures (ECACC): MCC13 (Cat#10092302), MCC14/2 (Cat#10092303), MCC26 (Cat#10092304), MS-1 (Cat#09111802), and MKL-1 (Cat#09111801).

Techniques:

Histological analysis of organotypic epithelial raft cultures (OERCs) of MCC cell lines co-cultured with primary human keratinocytes (PHKs). MCPyV − MCC cell lines (MCC14/2, MCC26 and MCC13) grow in OERCs (indicated by arrows in the H&E sections) when co-cultured with PHKs at a ratio of 1 to 5. Except MCC13, these cell lines express NCAM, a typical MCC marker. MCPyV + MCC cell lines (MS-1, MKL-1 and WAGA) also proliferate in our 3-D culture model, as indicated by full arrows in the H&E sections, when co-cultured with PHKs. These cells express the LT of MCPyV (although the murine fibroblasts stain positive as well, likely due to cross-reactivity to the murine antibody) and the MCC marker CK20, as confirmed by IHC. Double IHC staining for MCPyV LT (brown) and CK20 (red) show co-localization of these signals. PHKs differentiate in a normal epithelium (pointed out by dashed arrows). All images were taken at 20× magnification and the bars equal 100 μm.

Journal: Cancers

Article Title: Organotypic Epithelial Raft Cultures as a Three-Dimensional In Vitro Model of Merkel Cell Carcinoma

doi: 10.3390/cancers14041091

Figure Lengend Snippet: Histological analysis of organotypic epithelial raft cultures (OERCs) of MCC cell lines co-cultured with primary human keratinocytes (PHKs). MCPyV − MCC cell lines (MCC14/2, MCC26 and MCC13) grow in OERCs (indicated by arrows in the H&E sections) when co-cultured with PHKs at a ratio of 1 to 5. Except MCC13, these cell lines express NCAM, a typical MCC marker. MCPyV + MCC cell lines (MS-1, MKL-1 and WAGA) also proliferate in our 3-D culture model, as indicated by full arrows in the H&E sections, when co-cultured with PHKs. These cells express the LT of MCPyV (although the murine fibroblasts stain positive as well, likely due to cross-reactivity to the murine antibody) and the MCC marker CK20, as confirmed by IHC. Double IHC staining for MCPyV LT (brown) and CK20 (red) show co-localization of these signals. PHKs differentiate in a normal epithelium (pointed out by dashed arrows). All images were taken at 20× magnification and the bars equal 100 μm.

Article Snippet: The other MCC cell lines were obtained from the European Collection of Authenticated Cell Cultures (ECACC): MCC13 (Cat#10092302), MCC14/2 (Cat#10092303), MCC26 (Cat#10092304), MS-1 (Cat#09111802), and MKL-1 (Cat#09111801).

Techniques: Cell Culture, Marker, Staining, Immunohistochemistry