Journal: Asian Journal of Andrology

Article Title: Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion

doi: 10.4103/aja.aja_57_17

Figure Lengend Snippet: PGAM1 expression in prostate cancer tissues and prostate cancer cell lines. ( a ) The immunohistochemistry image of the whole TMA (scale bar = 1.5 mm). Representative immunohistochemistry images of PGAM1 protein expression from ( a ) with high intensity ( b ) in prostate cancer tissue (D7, scale bar = 0.2 mm), with intermediate intensity ( c ) in prostate cancer tissue (H7, scale bar = 0.2 mm), and with low intensity ( d ) in prostate cancer tissue (E4, scale bar = 0.2 mm). The percentage of PGAM1 positive cells in D7 is 90%, in H7 is 70%, and in E4 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 × 1 = 1. ( e ) Part of C14 from ( a ) with a magnification of 400× (scale bar = 50 μm). ( f ) Western blotting of PGAM1 protein expression in prostate cancer cell lines and normal prostate epithelial cell lines. PGAM1: phosphoglycerate mutase 1; TMA: tissue microarray.

Article Snippet: A human prostate tissue microarray (TMA; PR1921) was purchased from US Biomax, Inc. (Rockville, MD, USA).

Techniques: Expressing, Immunohistochemistry, Western Blot, Microarray

Journal: The FASEB Journal

Article Title: 12-HETER1/GPR31, a high-affinity 12( S )-hydroxyeicosatetraenoic acid receptor, is significantly up-regulated in prostate cancer and plays a critical role in prostate cancer progression

doi: 10.1096/fj.201500076

Figure Lengend Snippet: 12-HETER1 expression positively correlates with aggressiveness and progression of prostate tumors. A) Expression of 12-HETER1 in prostate carcinoma cells. Immunocytochemistry on HEK293 cells expressing 12-HETER1. a, b) Staining with 12-HETER1 antibody was optimized on HEK293 cells transfected with 12-HETER1 (a) or pcDNA (b) vector. Strong brown staining was detected in HEK293/12-HETER1 cells (a) compared to negative control (b). c, d) Immunohistochemistry to detect 12-HETER1 in 2 cases of human prostate cancer tissue. c) Benign gland (N, left corner, arrow) with rare cells showing positive staining, and neoplastic glands (T, GS-3/4, arrowhead) showing intense brown staining (arrowhead); original magnification, ×10. d) Strong staining in area (upper left, arrow) with mixed GS-3/4 tumor area, weak staining in area (lower right, arrowhead) with GS-3 tumor; original magnification, ×10. Images are representatives from 7 samples. B) Array analysis of 12-HETER1 expression. a, b) Immunohistochemistry to assay 12-HETER1 expression on 2 human prostate tissue microarrays. Expression of 12-HETER1 is significantly up-regulated in disease stage II/III human prostate tumor specimens (n = 8, P = 0.007, Student’s t test. c, d) Representative images of normal (c) and prostate tumor (d) specimens.

Article Snippet: Next we used qPCR to determine 12-HETER1 expression in 2 commercially available human prostate tissue microarrays from OriGene Technologies, HPRT101 and HPRT102 ( ).

Techniques: Expressing, Immunocytochemistry, Staining, Transfection, Plasmid Preparation, Negative Control, Immunohistochemistry

Journal: Molecular cancer research : MCR

Article Title: AXL is a Putative Tumor Suppressor and Dormancy Regulator in Prostate Cancer

doi: 10.1158/1541-7786.MCR-18-0718

Figure Lengend Snippet: AXL is not expressed in human prostate tumors. A, AXL expression prostate cancer patients who did and did not experience a biochemical recurrence (n = 1405 patients, GenomeDx GRID dataset). P value was calculated using the Wilcoxon rank test. B, Recurrence-free survival of patients with high (above the median) and low (below the median) AXL expression (n = 571 patients, Taylor et al, 2010 visualized and analyzed with Project Betastasis). C, Representative images of AXL IHC staining of a prostate tissue microarray; LN, lymph node. C42B cells served as a negative control, DU145 as a positive control. All images at original magnification x 200. D, AXL IHC staining in metastatic patient tissue; upper panel scale bar, 100 μm; lower panel scale bar, 50 μm.

Article Snippet: Human prostate tissue microarray (TMA) staining This study was approved by the Johns Hopkins institutional internal review board and followed the U.S. Common Rule.

Techniques: Expressing, Immunohistochemistry, Microarray, Negative Control, Positive Control