Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb attenuates OA progression. A . Representative images of H&E staining of synovium and cartilage, SO-FG and TB staining of mouse knee joints. Scale bar = 100 μm. n = 6. B . Synovitis score comparing the severity of synovial inflammation in mice. n = 6. C . OARSI scoring system comparing cartilage degradation in mice. n = 6. D . Representative IHC images of COL2A and MMP13 in mouse knee cartilage. Scale bar = 100 μm. n = 6. E . Quantitative analysis of COL2A-positive area in cartilage. n = 6. F . Quantitative analysis of MMP13-positive area in cartilage. n = 6. G . Representative micro-CT 3D reconstruction images of mouse knee joints and osteophytes (indicated by red arrows). n = 6. H . Quantitative analysis of osteophyte numbers. n = 6. I . Representative coronal micro-CT images of mouse knee joints. n = 6. J . Quantitative analysis of subchondral bone volume fraction (BV/TV) and trabecular thickness (Tb.Th). n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: Staining, Micro-CT

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb regulates chondrocyte function via synovial macrophages. A . Schematic diagram of the macrophage–chondrocyte co-culture system. B . Western blot analysis of COL2A, Aggrecan, MMP13, and ADAMTS5 protein levels in chondrocytes under different co-culture conditions, and the quantitative analysis of the protein. n = 3. C . Representative micromass Alcian blue staining of chondrocytes under different co-culture conditions. n = 3. D . Flow cytometry analysis of chondrocyte proliferation under different co-culture conditions, with quantitative analysis. n = 6. E . Representative EdU staining images and quantitative analysis of chondrocyte proliferation under different co-culture conditions. Scale bar = 100 μm. n = 6. F . Flow cytometry analysis of chondrocyte apoptosis under different co-culture conditions, with quantitative analysis. n = 6. G . TUNEL assay for chondrocyte apoptosis under different co-culture conditions, with quantitative analysis. Scale bar = 100 μm. n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: Co-Culture Assay, Western Blot, Staining, Flow Cytometry, TUNEL Assay

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb suppresses NLRP3 inflammasome-mediated macrophage inflammation and protects chondrocytes. A . Heatmap of RNA-seq analysis comparing Gb-treated and control macrophages. B . GSEA indicating significant reduction in cytokine activity and metalloproteinase activity in Gb-treated macrophages. C . GO enrichment analysis showing differential mRNAs are mainly involved in maintaining cell polarization, regulating inflammatory response, and regulating NLRP3 signaling. D . Western blot analysis of NLRP3 knockdown efficiency in macrophages. n = 3. E . IF analysis of NLRP3 knockdown efficiency. Scale bar = 100 μm. n = 6. F . Western blot analysis of Caspase-1 and IL-1β expression in shNLRP3 macrophages treated with Gb. n = 3. G . qRT-PCR analysis of IL-1β, TNF-α, and IL-10 mRNA in shNLRP3 macrophages treated with Gb. n = 3. H . Representative micromass Alcian blue staining of chondrocytes under different co-culture conditions. n = 3. I . Western blot analysis of COL2A, Aggrecan, MMP13, and ADAMTS5 protein levels in chondrocytes under different co-culture conditions. n = 3. J . Flow cytometry analysis of chondrocyte proliferation under different co-culture conditions, with quantitative analysis. n = 6. K . Representative EdU staining images and quantitative analysis of chondrocyte proliferation. Scale bar = 100 μm. n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: RNA Sequencing, Control, Activity Assay, Western Blot, Knockdown, Expressing, Quantitative RT-PCR, Staining, Co-Culture Assay, Flow Cytometry

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: In vivo knockdown of synovial macrophage NLRP3 weakens Gb-mediated protection against OA. A . Representative IF images of ZsGreen distribution in the knee joint after intra-articular injection of rAAV-shNLRP3, with quantitative analysis of ZsGreen-positive cells in synovium and cartilage. S indicates synovium, and C indicates cartilage. Scale bar = 1 mm (left), 50 μm (right). n = 3. B . Representative IHC images of NLRP3 in synovium and IF images of F4/80 and NLRP3 co-staining in synovium. Scale bar = 100 μm. n = 6. C . Representative HE, SO-FG, and TB staining of synovium and cartilage. Scale bar = 100 μm. n = 6. D . Synovitis score comparing severity of synovial inflammation. n = 6. E. OARSI score comparing cartilage degradation. n = 6. F . Representative IHC images of COL2A and MMP13 in cartilage. Scale bar = 100 μm. n = 6. G . Quantitative analysis of COL2A and MMP13 positive areas. n = 6. H . Representative micro-CT 3D reconstruction images showing osteophytes (red arrows) and quantitative analysis of osteophyte numbers. n = 6. I. Representative coronal micro-CT images, with quantitative analysis of subchondral BV/TV and Tb.Th. n = 6. Data are presented as mean ± SD. Student’s t-test was used for two-group comparisons, and one-way ANOVA for multiple-group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: In Vivo, Knockdown, Injection, Staining, Micro-CT

Journal: Scientific Reports

Article Title: A lncRNA and radiomics-based model for predicting the response of non-small cell lung cancer to chemo- and radio-therapy

doi: 10.1038/s41598-026-39560-x

Figure Lengend Snippet: ( A ) GO enrichment analysis of 1650 targeted mRNAs. ( B ) KEGG pathway enrichment analysis of 1650 targeted mRNAs . ( C ) The ceRNA network map of MIF-AS1. ( D ) Analysis of the correlation between MIF-AS1 expression and RAD21 expression in NSCLC tumors from the TCGA database (Pearson correlation analysis). ( E ) Knockdown of MIF-AS1 by siRNA decreases RAD21 expression in NSCLC PC9 cells. PC9 cells were transfected with control (si-NC) and MIF-AS1 siRNAs (si-MIF-AS1-1), and the mRNA and protein levels of RAD21 were determined by qPCR and western blot (Student’s t test). ( F ) KM survival analysis of RAD21 in the TCGA dataset. NSCLC patients were divided into low-expression and high-expression groups based on the optimal cut-off point.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST for 2 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies: anti-Rad21 rabbit monoclonal antibody (Absin, Shanghai, China; 1:1000) and anti-β-Actin mouse monoclonal antibody (Proteintech, Wuhan, China; 1:5000).

Techniques: Expressing, Knockdown, Transfection, Control, Western Blot

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the MMP‐2 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. MMP‐2 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the TNF‐α and IL‐6 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. (A) represents subsequent time points for TNF‐α analysis, and (B) represents subsequent time points for IL‐6 analysis. TNF‐α and IL‐6 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IL‐6: interleukin‐6; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the VEGF‐A levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. VEGF‐A levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; P 4 , progesterone; VEGF‐A, vascular endothelial growth factor‐A.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the IGFBP‐1 secretions in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser+ E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser + E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. IGFBP‐1 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IGFBP‐1, insulin‐like growth factor‐binding protein‐1; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification, Binding Assay