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Journal: Cell

Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills

doi: 10.1016/j.cell.2017.08.014

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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper Cat#N/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

Techniques: Recombinant, Sequencing, Software