mathiq Search Results


cultured cgns  (Millar Inc)
90
Millar Inc cultured cgns
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Cultured Cgns, supplied by Millar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thermal conductivity and thermal effusivity testing system tc-30  (Mathis Instruments Ltd)
90
Mathis Instruments Ltd thermal conductivity and thermal effusivity testing system tc-30
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Thermal Conductivity And Thermal Effusivity Testing System Tc 30, supplied by Mathis Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hot disktmtm  (Mathis Instruments Ltd)
90
Mathis Instruments Ltd hot disktmtm
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Hot Disktmtm, supplied by Mathis Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clearmix  (m-Tec Mathis Technik gmbh)
90
m-Tec Mathis Technik gmbh clearmix
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Clearmix, supplied by m-Tec Mathis Technik gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wheat straw hydrolysate 38 8  (ATCC)
90
ATCC wheat straw hydrolysate 38 8
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Wheat Straw Hydrolysate 38 8, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mathiq/Fusarium+oxysporum+f%2E+sp%2E+asparagi+Cohen%2C+anamorph/10__15835_slash_buasvmcn___fst_ascii58_2025__0036-120-118-140
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hot disk™  (Mathis Instruments Ltd)
90
Mathis Instruments Ltd hot disk™
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Hot Disk™, supplied by Mathis Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mathiq/hot+disk+/us06929751-582-16-20
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bt-01tm unit  (Mathis Instruments Ltd)
90
Mathis Instruments Ltd bt-01tm unit
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Bt 01tm Unit, supplied by Mathis Instruments Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mathiq/bt01tm+unit/10__1016_slash_j__apt__2018__03__031-232-6-9
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airless machine  (m-Tec Mathis Technik gmbh)
90
m-Tec Mathis Technik gmbh airless machine
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Airless Machine, supplied by m-Tec Mathis Technik gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mathiq/airless+machine/us07759416-120-7-7
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mathis dt  (Hirschmann)
86
Hirschmann mathis dt
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Mathis Dt, supplied by Hirschmann, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mathiq/dt+mathis/pm42082988-324-0-6
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pclx ubi venusn patrick salmon lab addgene  (Addgene inc)
93
Addgene inc pclx ubi venusn patrick salmon lab addgene
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Pclx Ubi Venusn Patrick Salmon Lab Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko thy1 1 vector  (Addgene inc)
93
Addgene inc plko thy1 1 vector
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Plko Thy1 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mathis et al814  (Stakich Inc)
90
Stakich Inc mathis et al814
Properties of IK(SO) in <t>CGNs.</t> A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly <t>inactivating</t> <t>component</t> in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.
Mathis Et Al814, supplied by Stakich Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Properties of IK(SO) in CGNs. A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly inactivating component in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.

Journal: The Journal of Neuroscience

Article Title: Modifying the Subunit Composition of TASK Channels Alters the Modulation of a Leak Conductance in Cerebellar Granule Neurons

doi: 10.1523/JNEUROSCI.3153-05.2005

Figure Lengend Snippet: Properties of IK(SO) in CGNs. A, Current traces taken from granule cells in the culture preparation (i), the wild-type slice preparation at P15 (ii) and P35 (iii), and a TASK-1 KO (-/-) slice preparation at P47 (iv). In each case, the holding potential was ramped from -20 to -160 mV and then maintained at -20 mV for 1 min. Note the absence of a slowly inactivating component in the data taken from a cultured granule cell (i). In the presence of this slowly inactivating current, IK(SO) was measured after the holding potential had been clamped at -20 mV for at least 1 min to avoid contamination by this conductance. B, Effect of altering external K+ concentration on the reversal potential of IK(SO). The current-voltage plot shows three traces recorded during a voltage-ramp protocol performed on the same adult wild-type granule cell. The predicted reversal potential, calculated from the Nernst equation, is marked with an open circle. C, Plot to compare the observed change in reversal potential (black filled circles) with the predicted values (open gray circles) calculated from the Nernst equation. This experiment illustrates that IK(SO) in CGNs is mediated by a pure K+ conductance. As expected from previous studies, this noninactivating K+ conductance is present in both the culture and the acute slice preparation.

Article Snippet: A pH-sensitive component of I K(SO) has been described in both cultured CGNs ( Watkins and Mathie, 1996 ; Millar et al., 2000 ; Han et al., 2002 ; Lauritzen et al., 2003 ) and cells in the acute slice preparation ( Millar et al., 2000 ; Brickley et al., 2001 ; Takayasu et al., 2003 ).

Techniques: Slice Preparation, Cell Culture, Concentration Assay