Journal: Journal of Translational Medicine

Article Title: The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype

doi: 10.1186/s12967-017-1256-y

Figure Lengend Snippet: Relationship between immune cell-specific markers

Article Snippet: For immunohistochemical (IHC) analysis of CD1a, CD68, CD163 and MARCO, 4 μm TMA-sections were pre- treated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the following antibodies: CD1a: clone NCL-CD1a-220, diluted 1:25, LEICA Biosystems, Newcastle, UK, CD68: clone KP1, diluted 1:1000, Dako, Glostrup, Denmark, CD163: clone 10D6 diluted 1:200 Novus Biologicals, Abingdon, United Kingdom, MARCO clone HPA063793, diluted 1:250, Atlas Antibodies, Bromma, Sweden.

Techniques:

Journal: Journal of Translational Medicine

Article Title: The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype

doi: 10.1186/s12967-017-1256-y

Figure Lengend Snippet: Cox proportional hazards analysis of the impact of investigated cell populations on overall survival according to morphology

Article Snippet: For immunohistochemical (IHC) analysis of CD1a, CD68, CD163 and MARCO, 4 μm TMA-sections were pre- treated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the following antibodies: CD1a: clone NCL-CD1a-220, diluted 1:25, LEICA Biosystems, Newcastle, UK, CD68: clone KP1, diluted 1:1000, Dako, Glostrup, Denmark, CD163: clone 10D6 diluted 1:200 Novus Biologicals, Abingdon, United Kingdom, MARCO clone HPA063793, diluted 1:250, Atlas Antibodies, Bromma, Sweden.

Techniques:

Journal: Nature Communications

Article Title: Toll-like receptor 4 and macrophage scavenger receptor 1 crosstalk regulates phagocytosis of a fungal pathogen

doi: 10.1038/s41467-023-40635-w

Figure Lengend Snippet: Baseline surface expression of a CD36 stained with anti-mouse CD36-BB515 antibody; b Macrophage Receptor with Collagenous structure (MARCO) stained with anti-mouse MARCO-Fluorescein antibody; and c Macrophage Scavenger Receptor 1 (MSR1), also known as CD204, stained with anti-mouse CD204-PE antibody on wildtype and Tlr4 −/− macrophages. Receptor expression was measured using flow cytometry. Data is representative of three independent experiments which gave similar results. Numbers above gates refer to the percentage of CD36-, MARCO- and MSR1-positive cells. d MPI cells, a non-transformed GM-CSF-dependent murine macrophage cell line, isolated from wildtype, Msr1 −/− , Marco −/− and MSR1/MARCO double knockout (DKO) mice, were infected with non-opsonised C. neoformans . The data shown is pooled from three independent experiments ( n = 9). Data is mean ± SEM; statistical significance was evaluated using a one-way ANOVA; P -values are shown above the graph. Source data are provided as a Source Data file.

Article Snippet: The following fluorochrome-conjugated antibodies were used: 0.5 μg/mL anti-mouse CD45-PerCP-Cyanine5.5 [ThermoFisher; Cat#: 45-0451-82; Clone 30-F11], 0.25 μg/100 μL anti-mouse CD204(MSR1)-PE [Fisher Scientific; Cat#: 12-204-682; Clone M204PA], 0.25 μg/100 μL anti-mouse CD36-BB515 [BD Biosciences; Cat#: 565933; Clone CRF D-2712], and 10 μL/100 μL anti-mouse MARCO-Fluorescein [Biotechne; Cat#: FAB2956F; Clone 579511].

Techniques: Expressing, Staining, Flow Cytometry, Transformation Assay, Isolation, Double Knockout, Infection