Journal: Frontiers in Neurology

Article Title: Induction of Osmolyte Pathways in Skeletal Muscle Inflammation: Novel Biomarkers for Myositis

doi: 10.3389/fneur.2018.00846

Figure Lengend Snippet: Messenger RNA levels in cultured CCL-136 cells treated for 24 h.

Article Snippet: RNA concentration was measured with a Nanodrop 1000 (ThermoFisher Scientific). cDNA was prepared from 200 ng of RNA with SuperScript II reverse transcriptase, 500 ng/μl oligo dTs, 0.1 M DTT, and 10 mM dNTPs each (Invitrogen, Darmstadt, Germany). cDNA was quantified through PCR reaction with Taqman Gene Expression Master mix (Applied Biosystems, Foster City, CA), using 6-carboxy-fluorescein-labeled probes and specific primers for: SLC5A3, Hs00272857_s1; SLC6A6, Hs00161778_m1; SLC6A12, HS00758246_m1; AKR1B1, Hs00739326_m1; NFAT5, Hs00232437_m1; MAPK14, Hs01051152_m1; RELA, Hs00153294_m1; NFKB1, Hs00765730_m1; NFKB2, Hs01028901_g1; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Hs99999905_m1 (Applied Biosystems).

Techniques: Cell Culture

Journal: Frontiers in Neurology

Article Title: Induction of Osmolyte Pathways in Skeletal Muscle Inflammation: Novel Biomarkers for Myositis

doi: 10.3389/fneur.2018.00846

Figure Lengend Snippet: Messenger RNA levels in cultured normal human myotubes treated for 24 h.

Article Snippet: RNA concentration was measured with a Nanodrop 1000 (ThermoFisher Scientific). cDNA was prepared from 200 ng of RNA with SuperScript II reverse transcriptase, 500 ng/μl oligo dTs, 0.1 M DTT, and 10 mM dNTPs each (Invitrogen, Darmstadt, Germany). cDNA was quantified through PCR reaction with Taqman Gene Expression Master mix (Applied Biosystems, Foster City, CA), using 6-carboxy-fluorescein-labeled probes and specific primers for: SLC5A3, Hs00272857_s1; SLC6A6, Hs00161778_m1; SLC6A12, HS00758246_m1; AKR1B1, Hs00739326_m1; NFAT5, Hs00232437_m1; MAPK14, Hs01051152_m1; RELA, Hs00153294_m1; NFKB1, Hs00765730_m1; NFKB2, Hs01028901_g1; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Hs99999905_m1 (Applied Biosystems).

Techniques: Cell Culture

Journal: Frontiers in Neurology

Article Title: Induction of Osmolyte Pathways in Skeletal Muscle Inflammation: Novel Biomarkers for Myositis

doi: 10.3389/fneur.2018.00846

Figure Lengend Snippet: Messenger RNA levels in cultured normal human myotubes treated with cytokines or added NaCl for up to 3 days.

Article Snippet: RNA concentration was measured with a Nanodrop 1000 (ThermoFisher Scientific). cDNA was prepared from 200 ng of RNA with SuperScript II reverse transcriptase, 500 ng/μl oligo dTs, 0.1 M DTT, and 10 mM dNTPs each (Invitrogen, Darmstadt, Germany). cDNA was quantified through PCR reaction with Taqman Gene Expression Master mix (Applied Biosystems, Foster City, CA), using 6-carboxy-fluorescein-labeled probes and specific primers for: SLC5A3, Hs00272857_s1; SLC6A6, Hs00161778_m1; SLC6A12, HS00758246_m1; AKR1B1, Hs00739326_m1; NFAT5, Hs00232437_m1; MAPK14, Hs01051152_m1; RELA, Hs00153294_m1; NFKB1, Hs00765730_m1; NFKB2, Hs01028901_g1; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Hs99999905_m1 (Applied Biosystems).

Techniques: Cell Culture

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Hawthorne leaf flavonoids prevent oxidative stress injury of renal tissues in rats with diabetic kidney disease by regulating the p38 MAPK signaling pathway

doi:

Figure Lengend Snippet: Immunochemical analysis of the (A) p38MAPK (B) and p-p38MAPK expression in the kidney of different groups (200×). Quantitative analysis of (C) p38MAPK and (D) p-p38MAPK expression in the kidney of different groups. The expression of p38MPAK and p-p38MAPK was significantly higher in the DKD group than in the HLF and IRB groups.

Article Snippet: Rabbit anti-rat p38MAPK and p-p38MAPK polyclonal antibodies were purchased from Boster (Beijing, China).

Techniques: Expressing

Journal: Malaria Journal

Article Title: Differential gene expression mediated by 15-hydroxyeicosatetraenoic acid in LPS-stimulated RAW 264.7 cells

doi: 10.1186/1475-2875-8-195

Figure Lengend Snippet: Select Gene Expression Changes Mediated by 15-HETE a

Article Snippet: Following RT, all assays were performed with Applied Biosystems TaqMan FAM labeled 20× probes: Arf3 (Taqman assay Mm00500194_m1), Cldn11 (Mm00500915_m1), Cxcl11 (Mm00444662_m1), Mapk14 (Mm00442497_m1), Prdx1 (Mm01621996_s1), Sdc1 (Mm00448918_m1), and Egr1 (Mm00656724_m1).

Techniques: Gene Expression, Binding Assay, Ubiquitin Proteomics, Virus, Transduction, Membrane, Activation Assay

Journal: Malaria Journal

Article Title: Differential gene expression mediated by 15-hydroxyeicosatetraenoic acid in LPS-stimulated RAW 264.7 cells

doi: 10.1186/1475-2875-8-195

Figure Lengend Snippet: Quantitative real-time RT-PCR validation of microarray results . RAW 264.7 cells were stimulated with 0.1 μg/mL LPS and treated with 40 μM 15-HETE for 24 h prior to RNA extraction. Fold-changes (treated stimulated cells relative to stimulated cells) are shown ( ± 99% confidence interval for quadruplicate measurements of n = 3 biological replicates). Abbreviations: Arf3 (ADP-ribosylation factor 3), Cldn11 (claudin 11), Cxcl11 (chemokine (C-X-C motif) ligand 11), Egr1 (early growth response 1), Mapk14 (mitogen-activated protein kinase 14), Prdx1 (peroxiredoxin 1), and Sdc1 (syndecan 1).

Article Snippet: Following RT, all assays were performed with Applied Biosystems TaqMan FAM labeled 20× probes: Arf3 (Taqman assay Mm00500194_m1), Cldn11 (Mm00500915_m1), Cxcl11 (Mm00444662_m1), Mapk14 (Mm00442497_m1), Prdx1 (Mm01621996_s1), Sdc1 (Mm00448918_m1), and Egr1 (Mm00656724_m1).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Microarray, RNA Extraction